Genetic mutations in endometrial cancer (EC) have been extensively studied in the Western population but not much in Asian cohorts. This study has demonstrated that PTEN and PIK3CA mutations are commonly found in EC among Malaysian women. Following RNA extraction from 20 cancerous and 18 non-cancerous tissues, the presence of mutations in 9 exons of PTEN and 3 exons of PIK3CA genes were detected using realtime PCR, accompanied by High Resolution Melt (HRM) analysis. Sequencing confirmed specificity of each PCR product. The mutations for both genes were detected in the samples with varying frequencies. Notably, all samples expressed mutation of PTEN at exon 7 but none in exon 4. Further analysis demonstrated that strong concurrent mutations occurred between exons 7 of PTEN with exon 20 region 1 of PIK3CA gene (90%). Our data showed mutations are present in EC and not the non-cancerous tissues. Larger samples are being collected to validate this observation.
Uterine Neoplasms

No abstract available.
Uterine Cervical Neoplasms*

No abstract available.
Classification* ; Uterine Cervical Neoplasms*

No abstract available.
Pregnancy* ; Uterine Cervical Neoplasms*

The clinical and histopathological characteristics were studied on 384 cases with invasive cervical cancer (ICC). The results have shown that high rate of ICC was found in the age range from 40 to 49 years. Hemorrhagic symptom is in highest rate (86.46%). Stage II A patient rate is high (30.99%) and the patients of stage II A and IIB are of more than haft of the patients (53.91%). The great majority of cases is nonkeratinisant large cell carcinoma (61.98%) and had strong stromal inflammatory reactions. The difference of stroma inflammatory reactions of histological types has statistical significance (p=0.014). Immunohistochemical stain for AE 1/AE 3 were positive in 96.29% of case and helpul in the identification of tumor cell differentiation.
Carcinoma ; Uterine Cervical Neoplasms

26,492 women from various regions, including 207 communes (203 from the North and the Middle of Vietnam, 4 from Can Tho province) and 3 hospitals (2 in Ha noi and 1 in Can Tho) have been examined (from 1990 to 1996) in order to detect early lessions of cervix cancer by cytological screening. The results are summarized respectively as follows: 1. Communes (on the whole): SIL all types (3.36%) among them LSIL (2.49%) and HSIL (0.87%); invasive carcinomas (0.029%). In Can Tho province (corresponding rates): 3.69% with LSIL (2.71%), HI|SIL (0.98%) and invasive carcinomas (0.06%). 2. Hospitals. Ha noi hospitals: SIL all types (5.62%) with HSIL (2.28%) and invasive carcinomas (0.05%). Can Tho hospital (respectively): 4.57%, 2.38% and 2.75%. The diffirences betwween the morbidity and the ASR (100,000 people) in the North and in the South are discussed.
Uterine Cervical Neoplasms ; diagnosis

No abstract available.
Humans ; Uterine Cervical Neoplasms

No abstract available.
Ulcer* ; Uterine Neoplasms*

No abstract available.
DNA* ; Uterine Cervical Neoplasms*

No abstract available.
Uterine Cervical Neoplasms*