1.Identification of miR-23a as a novel microRNA normalizer for relative quantification in human uterine cervical tissues.
Yuanming SHEN ; Yang LI ; Feng YE ; Fenfen WANG ; Xiaoyun WAN ; Weiguo LU ; Xing XIE
Experimental & Molecular Medicine 2011;43(6):358-366
Quantitative real-time RT-PCR (RT-qPCR) is being widely used in microRNA expression research. However, few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in microRNA RT-qPCR studies. The aim of this study was to identify the most stable reference gene(s) for quantification of microRNA expression analysis in uterine cervical tissues. A microarray was performed on 6 pairs of uterine cervical tissues to identify the candidate reference genes. The stability of candidate reference genes was assessed by RT-qPCR in 23 pairs of uterine cervical tissues. The identified most stable reference genes were further validated in other cohort of 108 clinical uterine cervical samples: (HR-HPV- normal, n = 21; HR-HPV+ normal, n = 19; cervical intraepithelial neoplasia [CIN], n = 47; cancer, n = 21), and the effects of normalizers on the relative quantity of target miR-424 were assessed. In the array experiment, miR-26a, miR-23a, miR-200c, let-7a, and miR-1979 were identified as candidate reference genes for subsequent validation. MiR-23a was identified as the most reliable reference gene followed by miR-191. The use of miR-23a and miR-191 to normalize expression data enabled detection of a significant deregulation of miR-424 between normal, CIN and cancer tissue. Our results suggested that miR-23a and miR-191 are the optimal reference microRNAs that can be used for normalization in profiling studies of cervical tissues; miR-23a is a novel microRNA normalizer.
Cervical Intraepithelial Neoplasia/diagnosis/genetics/*metabolism/pathology
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Cervix Uteri/*metabolism/pathology
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Early Detection of Cancer
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Female
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Gene Expression Profiling/*standards
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Humans
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MicroRNAs/genetics/*metabolism/standards
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Microarray Analysis
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Reference Standards
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Reverse Transcriptase Polymerase Chain Reaction
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Uterine Cervical Neoplasms/diagnosis/genetics/*metabolism/pathology
2.Distinction between lymphoma-like lesions and lymphoma of uterine cervix: a clinicopathologic study of 26 cases.
Jin-biao LU ; Xiao-qiu LI ; Xiong-zeng ZHU
Chinese Journal of Pathology 2007;36(5):297-301
OBJECTIVETo study the clinicopathologic features and differential diagnosis of lymphoma-like lesions and lymphoma of uterine cervix.
METHODSClinical data and hematoxylin and eosin-stained slides of 10 cases of lymphoma-like lesion and 16 cases of lymphoma of uterine cervix were reviewed. Immunohistochemical study for B- and T-cell markers and light chains (kappa, lambda) were performed on paraffin sections. The rearrangement status of immunoglobulin heavy chain (IgH) gene was analyzed with semi-nested polymerase chain reaction in 4 cases lymphoma-like lesion and 4 cases of lymphoma of uterine cervix.
RESULTSThe age of patients with lymphoma-like lesion ranged from 24 to 54 years (medium = 43 years). The lesion generally presented with cervical erosion or polyp. Microscopically, it is characterized by focal or diffuse superficial infiltration of immunoblast-like large B cells intermingled with a polymorphic population of inflammatory cells, including plasma cells, eosinophils and neutrophils. Maturation of the transformed large B cells was also noticed. On the other hand, the age of the patients with lymphoma of uterine cervix varied from 28 to 78 years (medium = 58 years). Cervical mass or diffuse enlargement of cervix were the commonest clinical findings. The cases included 12 examples of diffuse large B-cell lymphoma and 4 examples of follicular lymphoma. The former was characterized by a diffuse monomorphic population of large atypical lymphoid cells, while neoplastic follicles were identified in the latter. Neither polymorphic inflammatory infiltrates nor maturation phenomenon was found. The immunostaining for kappa and lambda light chains was inconclusive. Molecular study showed clonal rearrangement of IgH gene in all cases of cervical lymphoma, as well as 2 cases of lymphoma-like lesion.
CONCLUSIONSThe distinction between lymphoma-like lesion and lymphoma of uterine cervix depends primarily on the clinical and histopathologic features. Assay for rearrangement of IgH gene may be helpful in differential diagnosis, though monoclonality can be detected in some benign lesions as well.
Adult ; Aged ; Antigens, CD20 ; metabolism ; CD79 Antigens ; metabolism ; Diagnosis, Differential ; Female ; Gene Rearrangement, B-Lymphocyte, Light Chain ; Humans ; Immunoglobulin G ; genetics ; Lymphoma, Follicular ; genetics ; metabolism ; pathology ; Lymphoma, Large B-Cell, Diffuse ; genetics ; metabolism ; pathology ; Middle Aged ; Polyps ; genetics ; metabolism ; pathology ; Uterine Cervical Erosion ; genetics ; metabolism ; pathology ; Uterine Cervical Neoplasms ; genetics ; metabolism ; pathology ; Young Adult
3.Glandular neoplasia of cervix.
Chinese Journal of Pathology 2006;35(12):744-746
Carcinoembryonic Antigen
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metabolism
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Carcinoma in Situ
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immunology
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pathology
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virology
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Carcinoma, Ductal, Breast
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immunology
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pathology
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virology
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Cervical Intraepithelial Neoplasia
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immunology
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pathology
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virology
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DNA, Viral
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analysis
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Diagnosis, Differential
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Female
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Human papillomavirus 16
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genetics
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isolation & purification
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Humans
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Ki-67 Antigen
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metabolism
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Uterine Cervical Dysplasia
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immunology
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pathology
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virology
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Uterine Cervical Neoplasms
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immunology
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pathology
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virology
4.Evaluation of cobas 4800 high-risk HPV test as a tool in cervical cancer screening and cytology triage.
Wen CHEN ; Lu-lu YU ; Hong WANG ; Chun-jing FU ; Feng CHEN ; Yan-qing CAO ; Le-ni KANG ; Xun ZHANG ; Fang-hui ZHAO ; Li GENG ; Li YU
Chinese Journal of Oncology 2012;34(7):543-548
OBJECTIVETo evaluate the feasibility and reliability of cobas 4800 HPV test for cervical cancer screening and cytology referral.
METHODScobas 4800 HPV test and hybrid capture 2 (HC-2) were used to detect high risk HPV DNA in 670 specimens of liquid-based cytology collected from three hospitals. The agreement between cobas and HC-2 tests was assessed. HPV PCR detection (HybriBio) and gene sequencing were used for genotyping, and the agreement of HPV16 and 18 genotyped by cobas and HybriBio was evaluated. Histological diagnosis was considered as a gold standard to estimate the sensitivity and specificity of cobas vs. HC-2 in detecting CIN2(+) in cervical lesions.
RESULTSThe crude agreement between cobas and HC-2 tests was 89.40%, the Kappa value was 0.778, the positive concordance rate was 86.42%, and the negative concordance rate was 91.36%. The crude agreement rates between cobas and HybriBio on HPV16 and 18 were 88.89% and 94.94%, the Kappa values were 0.777 and 0.753, the positive concordance rates were 98.91% and 100.00%, and the negative concordance rates were 78.41% and 94.44%, respectively. HPV PCR detection (HybriBio) and gene sequencing were considered as adjusted standard: the high risk HPV positive concordance rate was 100%, negative coincidence rate was 94.42%, HPV16 and 18 positive concordance rates were both 100%, and negative concordance rates were 82.35% and 94.44%, respectively. Regarding the detection of CIN2(+), the sensitivity and specificity were 91.07% and 70.97% for cobas, and 93.75% and 71.33% for HC-2, with a non-significant difference between the results of the two tests (P > 0.05).
CONCLUSIONScobas4800 HPV test has good screening sensitivity and specificity in correct detection of HPV16 and 18 and other high-risk HPV virus types.
Adult ; Aged ; Aged, 80 and over ; Cervical Intraepithelial Neoplasia ; diagnosis ; pathology ; virology ; Cytodiagnosis ; methods ; DNA, Viral ; metabolism ; Early Detection of Cancer ; methods ; Female ; Genotype ; Human papillomavirus 16 ; genetics ; Human papillomavirus 18 ; genetics ; Humans ; Mass Screening ; methods ; Middle Aged ; Papillomavirus Infections ; Sensitivity and Specificity ; Triage ; Uterine Cervical Neoplasms ; diagnosis ; pathology ; virology ; Young Adult