1.Deuteromethylactin B from a Freshwater-derived Streptomyces sp..
Anam F SHAIKH ; Maryam ELFEKI ; Samantha LANDOLFA ; Urszula TANOUYE ; Stefan J GREEN ; Brian T MURPHY
Natural Product Sciences 2015;21(4):261-267
Compared to their terrestrial and marine counterparts, little is known about the capacity of freshwater-derived actinomycete bacteria to produce novel secondary metabolites. In the current study, we highlight the disparities that exist between cultivation-independent and -dependent analyses of actinomycete communities from four locations in Lake Michigan sediment. Furthermore, through phylogenetic analysis of strains isolated from these locations, we identified a Streptomyces sp., strain B025, as being distinct from other Streptomyces spp. isolated from sediment. Upon fermentation this strain produced a rare class of eight-membered lactone secondary metabolites, which have been for their antitumor properties. We used spectroscopic and chemical derivitization techniques to characterize octalactin B (1) in addition to its corresponding novel, unnatural degradation product (2).
Bacteria
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Fermentation
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Fresh Water
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Lakes
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Michigan
;
Streptomyces*