BACKGROUND: JEOL BioMajesty JCA-BM6010/C (JCA-BM6010/C, JEOL Ltd., Japan) is a recently developed ultra-compact automated clinical chemistry analyzer with a throughput of 1,200 tests per hour. Here, we present the first performance evaluation of JCA-BM6010/C. METHODS: We evaluated the precision, linearity, correlation, accuracy, and carryover of 11 analytes (ALP, ALT, AST, calcium, creatinine, GGT, glucose, LDH, total bilirubin, total protein, and uric acid) using the JEOL closed reagent (JEOL Ltd.) according to the guidelines of the Clinical Laboratory Standards Institute. Linearity was further evaluated for ALT, AST, and GGT using open reagents by Sekisui (Japan). The performance of JCA-BM6010/C was compared to that of the Roche-Hitachi Cobas 8000 c702 chemistry autoanalyzer (Cobas 8000, Roche Diagnostics, Switzerland). Its performance using open reagents from Denka Seiken (Japan), Roche, and Sekisui was also evaluated. RESULTS: The total coefficients of variation (CV) for all analytes were 1.0–2.7%. Linearity was observed for all analytes over the entire tested analytical range (R²≥0.99). The results of JCA-BM6010/C strongly correlated (r≥0.988) with those of Cobas 8000 for all evaluated analytes except LDH (r=0.963), as well as for all open reagents. Recovery rates for creatinine, glucose, calcium, and uric acid were 96.6–101.5% and 98.7–109.3% with the JEOL exclusive and open reagents, respectively. Sample carryover was less than 0.34%. CONCLUSIONS: JCA-BM6010/C showed acceptable performance in the precision, linearity, correlation, accuracy, and sample carryover analyses and in the method comparison. Therefore, it could be a useful routine laboratory medical analyzer.
Bilirubin ; Calcium ; Chemistry ; Chemistry, Clinical* ; Creatinine ; Glucose ; Indicators and Reagents ; Methods ; Uric Acid

Based on Ag nanoparticles as the surface-enhanced Raman spectroscopy (SERS)-active nanostructure, the SERS of uric acid was presented in the paper. The absorption spectroscopies of uric acid and the mixture of silver colloids and uric acid were measured. The possible enhancing mechanism of the uric acid on silver colloid was speculated. The characteristic SERS bands of uric acid were tentatively assigned. The influence of absorption time and different ion on the SERS of uric acid were also discussed. The SERS spectral intensity changes linearly with the uric acid concentration, which indicated that the SERS might provide a new kind of direct and fast detecting method for the detection of uric acid. The detection limit of uric acid in silver sol is found to be 1 mg/L.
Metal Nanoparticles ; chemistry ; Silver ; chemistry ; Spectrum Analysis, Raman ; methods ; Surface Properties ; Uric Acid ; analysis

Laboratory values change with age and interpreting laboratory results from elderly people using the reference intervals for younger adults may not be appropriate. The authors investigated the distribution patterns of routine chemistry values from elderly people to determine whether current reference intervals are also valid for elderly people. A total of 1,215 persons older than 65 years and 1,827 healthy adults below 65 years of age were evaluated. Blood samples were collected after an overnight fast and analyzed for chemistry tests. Computing the central 95th percentile showed that the total protein, albumin, ALP, LD, creatinine, uric acid, triglyceride, HDL-cholesterol, and electrolytes of elderly people were within the standard reference intervals used in our laboratory. For AST and ALT, the upper range of the central 95th percentile in the elderly population was found to be outside the common reference interval. However, the central 90th percentile values of AST and ALT were compatible with the common reference intervals. GGT, BUN, total cholesterol, LDL-cholesterol, and glucose showed higher values than the upper limits of the reference intervals. For common clinical chemistry tests, the common reference values in general should be applicable to elderly people, even though some parameters showed wider distributions in the elderly.
Adult ; Aged ; Chemistry, Clinical ; Cholesterol ; Clinical Chemistry Tests ; Creatinine ; Electrolytes ; Glucose ; Humans ; Reference Values ; Uric Acid

BACKGROUND: Results of automated clinical chemistry tests are affected by many factors including analytical variability. In 1976, the College of American Pathologists (CAP) Conference on the analytical goals in clinical chemistry recommended that analytical variability should be less than 1/4 of the appropriate biological variability to improve distinction between normal and diseased populations. This study is conducted to evaluate whether automated clinical chemisty analyses performed in our laboratory is in conformance with the CAP's recommendation. METHODS: Routine chemistry and electrolyte tests were performed using Hitachi 747 automatic analyzer on 22 healthy volunteers. Blood samples were obtained from the volunteers' same vein twice in one week interval to study the total variability. Serum samples from 12 subjects were tested in duplicate immediately after blood collection for within-run analytical variability; and samples from another 10 subjects were repeated after 6 hours for within-day analytical variability. Within-run analytical variability plus within-day analytical variability make total analytical variability. Biological variability was defined as the difference between total variability and the analytical variability. Finally, ratios of analytical and biological variabilities were calculated. RESULTS: The ratios of analytical and biological variabilities of uric acid, glucose, and K were less than 0.25. But ratios of BUN, PO4, alkaline phosphatase, total bilirubin, AST, cholesterol, ALT, Cl, and protein exceeded 0.25. The ratios of Na, Ca, albumin, CO2, and creatinine could not be calculated. CONCLUSIONS: It is suggested that the analytical processes of the automated clinical chemistry tests be improved so as to be in conformity with the CAP's recommendation.
Alkaline Phosphatase ; Bilirubin ; Chemistry ; Chemistry, Clinical* ; Cholesterol ; Clinical Chemistry Tests* ; Creatinine ; Glucose ; Healthy Volunteers ; Uric Acid ; Veins

The antihypertensive effect of Indapamide(Fludex(R)) was studied in 31 patients of essential hypertension and following results were obtained. 1) Daily dosage was 1mg b.i.d. and total duration of medication was weeks. 2) Mean systolic and diastolic blood pressure declined by 23mmHg(14%) and 18mmHg(17%) respectively. 3) Good or fair controls were achieved in 78% of patients. 4) There was no significant change in heart rate during and after treatment. 5) There were no significant changes in fasting blood sugar, serum creatinine, K+, uric acid, ca++, transaminase and cholesterol levels before and after treatment. 6) In 5 patients transient side effects were observed which resolved spontaneously. In view of these results Indapamide appears to be effective agent for the treatment of mild to moderate hypertension and dose not cause significant change in blood chemistry.
Blood Glucose ; Blood Pressure ; Chemistry ; Cholesterol ; Creatinine ; Fasting ; Heart Rate ; Humans ; Hypertension ; Indapamide ; Uric Acid

Six trials with 3 samples for each of external quality assessment for general chemistry and blood gas were performed in 2007. All the control materials were sent in specifically-made boxes at the same time. The response rates were 92.0% in general chemistry and 95.5% in blood gas. The items included sodium, potassium, chloride, BUN, glucose, calcium, phosphorus, uric acid, creatinine, bilirubin, total protein, albumin, total cholesterol, triglyceride, AST, ALT, ALP, LD and GGT in general chemistry and pH, pCO2 and pO2 in blood gas. Compared with the previous year (2006), the methods of analysis were slightly changed and the coefficient of variation and VIS scores of general chemistry items were not significantly changed.
Bilirubin ; Calcium ; Chemistry, Clinical ; Cholesterol ; Creatinine ; Glucose ; Hydrogen-Ion Concentration ; Korea ; Phosphorus ; Potassium ; Sodium ; Uric Acid

BACKGROUND: The Rx Imola (Randox, UK) is newly released bench top - fully automated analyzer based on Window XP software with high-throughput (640 tests per hour with ISE) and continuous random access. We evaluated the performance of Rx Imola for the routine chemistry. METHODS: Repeatability (within-day precision), between-day precision, within-device precision, linearity, recovery rates and correlation were evaluated for 19 items including AST, ALT, ALP, GGT, total bilirubin, calcium, phosphorus, albumin, total protein, BUN, creatinine, glucose, amylase, total cholesterol, triglyceride, HDL, LDH, CK and uric acid. Commercialized quality control materials and patient's sera were used. For correlation study, 747-100 (HITACHI, Japan) and VITROS 950 (Ortho-Clinical Diagnostics, USA) were used as comparative analyzers. RESULTS: Coefficients of variation (CVs) of all items in repeatability and between-day precision study were below 5%. The linearities were statistically acceptable (R2>0.99) for all items. The recovery rates ranged from 95.7 to 105.3%. The comparison study showed high correlation between Rx Imola and 747-100 or VITROS 950. Correlation coefficients of all items were above 0.99 except HDL and albumin. CONCLUSIONS: This study showed satisfactory results in precision, linearity, recovery rates and comparison studies of Rx Imola. It was expected to be useful for routine chemistry analysis and back up, because of high performance, easy handling and small size.
Amylases ; Bilirubin ; Calcium ; Chemistry* ; Cholesterol ; Creatinine ; Glucose ; Phosphorus ; Quality Control ; Statistics as Topic ; Triglycerides ; Uric Acid

A patented kinetic uricase method was evaluated for serum uric acid assay. Initial absorbance of the reaction mixture before uricase action (A(0)) was obtained by correcting the absorbance at 293 nm measured before the addition of uricase solution, and background absorbance (A(b)) was predicted by an integrated method. Uric acid concentration in reaction solution was calculated from A, the difference between A(0) and A(b), using the absorptivity preset for uric acid. This kinetic uricase method exhibited CV<4.3% and recovery of 100%. Lipids, bilirubin, hemoglobin, ascorbic acid, reduced glutathione and xanthine <0.32 mmol/L in serum had no significant effects. A linearly responded to 1.2 to 37.5 micromol/L uric acid in reaction solution containing 15 microl serum. The slope of linear response was consistent with the absorptivity preset for uric acid while the intercept was consistent with that for serum alone. Uric acid concentrations in clinic sera by different uricase methods positively correlated to each other. By Bland-Altman analysis, this kinetic uricase method accorded with that by quantifying the total change of UV absorbance on the completion of uricase reaction. These results demonstrated that this kinetic uricase method is reliable for serum uric acid assay with enhanced resistance to both xanthine and other common errors, wider range of linear response and much lower cost.
Humans ; Kinetics ; Reagent Kits, Diagnostic ; Spectrophotometry, Ultraviolet ; Urate Oxidase ; chemistry ; Uric Acid ; blood ; metabolism

BACKGROUND: Hitachi 7600-110 (Hitachi, Tokyo, Japan) is an automated chemistry analyzer introduced in 1999. It consists of one dispensing type plus one pipetting type module and one electrolyte analyzer. We evaluated the performance of the analyzer in order to access the utility of the Hitachi 7600-110 autoanalyzer for the efficiency of routine chemistry work. METHODS: Within-day and between-day precision, accuracy, linearity, and recovery rates for albumin, ALP, ALT, AST, BUN, Ca, cholesterol, creatinine, r-GT, glucose, phosphorus, total bilirubin, TG, total protein, uric acid, CK, direct bilirubin, Fe, HDL-cholesterol, LD, Mg, Cl, K, and Na (24 items) were evaluated. Commercialized quality control material, Lyphochek (Bio-Rad, CA, USA) and patient sera for test specimens were used. Vitros 750 (Johnson & Johnson, Rochester, USA) was used as a control analyzer to evaluate the correlation. RESULTS: The within-day coefficients of variations (CVs) of almost all items were less than 3.0% except for direct bilirubin (7.71%), Mg (6.65%), and Fe (5.83%). The between-day CVs of almost all items were less than 5% except the direct bilirubin (13.16%). All items showed good linearity in the performance range (r>0.99, slope, 0.97-1.0). The results of ALT, AST, BUN, creatinine, glucose, CK, LD, Cl, K, and Na between Hitachi 7600-110 and the Vitros 750 revealed good correlation (r> or = 0.975) except Na and Cl. Hitachi data of Na and Cl showed significantly lower results (P<0.05) than did the Vitros 750 data. The recovery rates for BUN, creatinine, glucose, and uric acid were in the range of 100.00 to 101.78%. CONCLUSIONS: Hitachi 7600-110 showed acceptable performance in the "within-day" and "between-day" precision, linearity, and accuracy. The Hitachi 7600-110 that combines dispensing and pipetting modules can improve the speed and the efficiency of the workflow and can minimize the reagent consumption and the cost of system management. Therefore, it can be used both in large laboratories in general hospitals with many examinations and in small laboratories with relatively small numbers of cases.
Bilirubin ; Chemistry* ; Cholesterol ; Creatinine ; Glucose ; Hospitals, General ; Humans ; Phosphorus ; Quality Control ; Uric Acid

BACKGROUND: Prompt and accurate urine chemistry analysis is important to provide information for diagnosis and therapy. In this study, we evaluated the overall performance and utility of an automated chemistry analyser for urine chemistry testing in accordance with Clinical and Laboratory Standards Institute guidelines. METHODS: From January 2015 to March 2015, we evaluated the precision, linearity, limits of detection, carryover, and turnaround times after automation of nine items: total protein, albumin, glucose, blood urea nitrogen, total calcium, magnesium, inorganic phosphate, creatinine, and uric acid. A Hitachi 7600-110 instrument (Hitachi Ltd., Japan) and Hitachi ID Privileged Access Manager (Hitachi Ltd.) were used for automated chemistry analysis and sample preparation, respectively. RESULTS: Regarding precision, the coefficient of variation was 3.9% to 1.6% for high levels and 3.3% to 24.1% for low levels. The linearity and coefficients of determination of all the test items were acceptable. Performance comparison revealed that the two systems were comparable, as evidenced by correlation coefficients >0.975 for most items; moreover, carryover of all items was <1%. The mean turnaround time was 59 minutes. CONCLUSIONS: Urine chemistry testing can be performed with acceptable precision, linearity, and performance by using the Hitachi 7600-110 automated chemistry analyser. The sample preparation system reduces turnaround time, which enhances the clinical utility of urine chemistry testing.
Automation ; Blood Glucose ; Calcium ; Chemistry* ; Creatinine ; Diagnosis ; Limit of Detection ; Magnesium ; Nitrogen ; Urea ; Uric Acid