Animals ; Antimetabolites, Antineoplastic ; adverse effects ; Fluorouracil ; adverse effects ; Male ; Rats ; Spermatozoa ; abnormalities

Diabetic Neuropathies ; diagnosis ; physiopathology ; Humans ; Skin ; innervation ; Sympathetic Nervous System ; physiology

AIMTo observe the effect of tamoxifen citrate on spermatogenesis and tubular morphology in rats.

METHODSThe effect of tamoxifen citrate i.g. at doses of 400 and 800 mg.kg(-1).day(-1) in 0.1 mL olive oil for 30 days on seminiferous tubular morphology, seminiferous epithelial diameter (STD), epithelial height (SEH), epididymal sperm count and percent abnormal sperm were evaluated at day 1, 12 and 36 after treatment. Controls were given the vehicle.

RESULTSThe higher dose resulted in tubular atrophy on day 31. The STD, SEH and sperm count were decreased and the abnormal spermatozoa increased in a dose-dependent manner with the maximal effect on day 36.

CONCLUSIONTamoxifen citrate induces tubular shrinkage and atrophy and sperm abnormality at a dose-dependent manner.

Animals ; Epithelial Cells ; cytology ; drug effects ; Male ; Rats ; Seminiferous Tubules ; anatomy & histology ; drug effects ; Sperm Count ; Spermatogenesis ; drug effects ; physiology ; Tamoxifen ; pharmacology

AIMTo evaluate the effect of tamoxifen citrate on male reproductive system of rat.

METHODSGroups of male rats were gavaged with tamoxifen at doses of 200 mg x kg(-1) x d(-1), 400 mg x kg(-1) x d(-1) or 800 mg x kg(-1) x d(-1) in 0.1 mL olive oil for 10 consecutive days. Controls were treated with 0.1 mL olive oil. Rats were anesthetized and killed on d 3, d 15 or d 35 after the last dose. Testes were collected, processed for paraffin embedding, sectioned at 5 microm thickness, stained with HE and analyzed microscopically.

RESULTSThere was a dose-dependent increase in the occurrence of seminiferous tubular distortion with germinal cell sloughing. The highest dose increased the number of multinucleated giant cells on d 3 and d 15.

CONCLUSIONTamoxifen citrate induces multinucleated giant cells and germinal epithelial sloughing in a dose-dependent manner and these changes are detrimental to male fertility.

Animals ; Antineoplastic Agents, Hormonal ; toxicity ; Dose-Response Relationship, Drug ; Giant Cells ; drug effects ; ultrastructure ; In Vitro Techniques ; Male ; Rats ; Rats, Wistar ; Seminiferous Tubules ; cytology ; drug effects ; Tamoxifen ; toxicity ; Testis ; cytology ; drug effects