2.Population analysis of Aedes albopictus (Skuse) (Diptera:Culicidae) under uncontrolled laboratory conditions.
H Nur Aida ; A Abu Hassan ; A T Nurita ; M R Che Salmah ; B Norasmah
Tropical biomedicine 2008;25(2):117-25
A semi laboratory experiment using 3 cohorts of Aedes albopictus adults was performed to obtain age-specific mortality and fecundity information and to derive statistical estimates of some population growth parameters. Life expectancy was calculated for both males and females. The following population parameters were estimated: intrinsic rate of increase (rm= 0.21), net reproductive (replacement) rate (Ro= 68.70), age at mean cohort reproduction (To=10.55 days), birth rate (B=0.23), death rate (D=0.02) and generation time (G=20.14 days). The high rm/B (0.91) and B/D (11.50) ratios indicated the high colonizing ability of Ae. albopictus in nature.
Upper case Bee
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Aedes albopictus
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Upper case dee
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Population
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Culicidae
3.Chlamydia trachomatis infection and distribution of serovars in the Eastern Highlands Province, Papua New Guinea.
Dagwin L Suarkia ; Charles S Mgone ; Deborah Lehmann ; Megan E Passey ; Tony Lupiwa ; Michael M Paniu ; Jacinta Kono ; Mexy Kakazo ; William Yeka ; Michael P Alpers
Papua and New Guinea medical journal 2007;50(3-4):134-44
We have used nested polymerase chain reaction (PCR) and the PCR-based endonuclease digestion method to genotype Chlamydia trachomatis serovars in 460 infected individuals from the Eastern Highlands Province of Papua New Guinea. Our study groups comprised women who presented in labour to the Goroka Base Hospital, their newborn infants, symptomatic children who presented to the hospital's Outpatients Department and men and women from 15 randomly selected villages in the Asaro Valley. In this analysis, the major outer membrane protein (MOMP) gene, omp1, of C. trachomatis was amplified using DNA obtained from the endocervix of women, urine from men, and both the eye and nasopharynx of children. Amplified DNAs were digested concurrently using Alul and a combination of EcoRI, Hinl and Hpall restriction enzymes. The mixtures were separated on electrophoretic gels and the respective serovars designated on the basis of resolved digested DNA patterns. Our results, which were confirmed also by omp1 sequence data, show serovars D, E, F, G, H and L3 to be present in the studied communities. The overall relative frequencies of these serovars were 30%, 21%, 25%, 1%, 20% and 2% respectively, with serovars D, E, F and H accounting for 97% of these infections. Double infections among these principal serovars were also detected in all our study groups but at a low overall frequency of 3%. Serovar D was the major agent involved in the aetiology of chlamydial infection in both children and adults though serovar F was the most frequent in newborn infants. Serovar H was relatively less frequent in symptomatic children. No trachoma-related serovars were detected, confirming the rarity of this disease in Papua New Guinea. In contrast, although clinical cases of lymphogranuloma venereum have not been described in the country, the detection of serovar L3 in this study suggests that it may occur. However, the association of L3 also with childhood infection indicates that it may be causing the same pathology as the serovars D-K that are associated with non-ulcerative sexually transmitted infections.
Upper case dee
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Papua New Guinea
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Child
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upper case aitch
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Upper case eff
5.Prevalence of intestinal protozoa in an aborigine community in Pahang, Malaysia.
M Y Noor Azian ; Y M San ; C C Gan ; M Y Yusri ; Y Nurulsyamzawaty ; A H Zuhaizam ; M N Maslawaty ; I Norparina ; I Vythilingam
Tropical biomedicine 2007;24(1):55-62
The objective was to estimate the prevalence of intestinal protozoa among the aborigines and to determine the problems regarding the infection. The study was carried out in January 2006 in Pos Senderut, Pahang, Malaysia. Samples of faeces were collected from children and adults and these were fixed in PVA and trichrome staining was carried out. From the 130 individuals studied, 94 (72.3%) were positive with at least one intestinal protozoa. Nine intestinal protozoa namely Blastocystis hominis, Giardia lamblia, Entamoeba histolytica, Entamoeba coli, Endolimax nana, Entamoeba hartmani, Entamoeba polecki, Iodamoeba butschlii and Chilomastix mesnili were detected. The prevalent species were B. hominis (52.3%), followed by G. lamblia (29.2%), E. coli (26.2%) and E. histolytica (18.5%). The other species ranged from 1.5 to 10.8%. Among the positive samples, mixed infection with E. histolytica and G. lamblia was 3.8%, E. histolytica and B. hominis was 15.4%, G. lamblia and B. hominis was 17.7%. Triple infection of E. histolytica, G. lamblia and B. hominis was 3.1%. The infection was more prevalent in children below 10 years age group (45.4%) and lowest in the age above 60 years (3.8%). The high prevalence was attributable to poor environmental management, poor personal hygiene and lack of health education.
Protozoal
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upper case gee
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Upper case ee
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Upper case Bee
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Infection as complication of medical care
7.Cross-sectional characterization of malaria in Sanma and Shefa Provinces, Republic of Vanuatu: malaria control implications.
Jason D Maguire ; Michael J Bangs ; Leonard Brennan ; Karl Rieckmann ; George Taleo
Papua and New Guinea medical journal 2006;49(1-2):22-31
Endemic malaria still exists in the Republic of Vanuatu, an 80-island archipelago that sits astride the southeast margin of the Southeast Asian-Melanesian malaria band (Buxton Line 170 degrees E, 20 degrees S). The annual parasite incidence has decreased dramatically over the past decade, which has been attributed to an intensive insecticide-treated bednet distribution program and implementation of a revised Plasmodium falciparum treatment policy that employs combination chloroquine + sulfadoxinelpyrimethamine as a first-line therapy. Standard malariometric surveys were conducted at 10 locations in 2 provinces, screening 2351 adults and children towards the end of the peak transmission season. Spleen rates were consistent with mesoendemic malaria. Examination of blood slides revealed a mean slide-positive rate of 22% (range 4% to 33%). P. falciparum predominated, accounting for 73% of infections, followed by P. vivax (25%). Among 396 individuals with P. falciparum, the gametocyte rate was 54%, with 37% presenting gametocytes alone without asexual stages. Only 8% and 4% of persons with asexual stage P. falciparum and P. vivax parasitaemia, respectively, were symptomatic. These data suggest that malaria transmission has increased in some locations in Vanuatu over the past decade and this report underscores the importance of appropriate bednet use and vector control in this setting as well as the impact of adding sulfadoxine/pyrimethamine and removing primaquine from the national malaria treatment formulary.
Malaria
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upper case pea
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Vanuatu
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control
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asexual
10.A new record of Fannia prisca Stein, 1918 (Diptera:Fanniidae) from peninsular Malaysia.
Chong Chin Heo ; Hiromu Kurahashi ; Kazumi Nishida ; Hwa Tan Siew ; Zulqarnain Mohamed ; Abdullah Marwi Mohamed ; John Jeffrey ; Baharudin Omar
Tropical biomedicine 2008;25(3):254-6
Fannia prisca Stein, 1918 is newly recorded from peninsular Malaysia. This record is based on 4 male specimens from Mount Berembun, Brinchang, Cameron Highland, Pahang state, peninsular Malaysia. It is previously recorded from China, Mongolia, Korea, Japan, Taiwan, Bonin Island, Thailand and oriental region. The male of Fannia prisca can be differentiated from male Fannia scalaris by the following features: for F. prisca, mid-coxa without spine; mid-tibia normal or without stout triangular ventral projection; and hind tibia usually with 2 av, while F. scalaris has several stout hook-like spines on the anterior margin; mid-tibia with stout triangular ventral projection and hind tibia usually with 3 av. Both F. prisca and F. scalaris can be differentiated from Fannia leucosticta by looking at its hind tibia, which only has 1 av.
Tibia
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Fannia
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Upper case eff
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Malaysia
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Stout