We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.
Animals ; Base Sequence ; China ; Clonorchis sinensis/enzymology/*genetics ; Comparative Study ; DNA, Helminth/chemistry/*genetics ; DNA, Mitochondrial/chemistry/*genetics ; DNA, Ribosomal/chemistry/genetics ; DNA, Ribosomal Spacer/chemistry ; Electron Transport Complex IV/genetics ; Genetic Markers ; Korea ; Molecular Sequence Data ; RNA, Ribosomal, 18S/genetics ; Research Support, Non-U.S. Gov't ; Sequence Alignment ; Species Specificity ; *Variation (Genetics)

To determine the molecular phylogenic location of Plagiorchis muris, 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome C oxidase subunit I (mtCOI) were sequenced and compared with other trematodes in the family Plagiorchiidae. The 28S D1 tree of P. muris was found to be closely related to those of P. elegans and other Plagiorchis species. And, the mtCOI tree also showed that P. muris is in a separate clade with genus Glypthelmins. These results support a phylogenic relationship between members of the Plagiorchiidae, as suggested by morphologic features.
Animals ; Base Sequence ; DNA, Helminth/chemistry/genetics ; DNA, Mitochondrial/chemistry/genetics ; Electron Transport Complex IV/chemistry/*genetics ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction ; RNA, Ribosomal, 28S/chemistry/*genetics ; Sequence Alignment ; Support, Non-U.S. Gov't ; Trematoda/classification/*genetics

Ninety cases of meniscus injury were treated by arthroscopic meniscetomy between March, 1988 and March, 1992. Eighty four cases were treated by arthroscopic partial, 5 cases by subtotal, and 1 case by total meniscectomy. All cases were followed up for more than 1 year. The results were as follows. 1. The ratio of male to female was 57 to 31, and that of right to left was 52 to 38. 2. There were 41 cases of medial meniscus and 49 cases of lateral meniscus. Longitudinal tears were 35 cases (39%), oblique tears were 20 cases (22%), horizontal tears were 15 cases (17%), and radial tears were 9 cases (10%). There was no difference in the shape of tear between medial and lateral meniscus. 4. The most common associated injury was anterior cruciate ligament tear. 5. The Tapper and Hoover scale was used to record the clinical results, and satisfatory results (exellent and good)were in 86% of cases.
Anterior Cruciate Ligament ; Clinical Study ; Female ; Humans ; Knee ; Male ; Menisci, Tibial ; Tears

No abstract available.
Cholesterol* ; Granuloma*

No abstract available.
Rhinoplasty*

We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones and 5 gallbladder (GB) stones. The patients who had the CBD stones suffered from cholangitis, and the patients with GB stones showed acute cholecystitis, respectively. The 28S and 18S rDNA genes were amplified successfully from 3 and/or 1 common bile duct stone samples, and then cloned and sequenced. The 28S and 18S rDNA sequences were highly conserved among isolates. Identity of the obtained 28S D1 rDNA with that of Clonorchis sinensis was higher than 97.6%, and identity of the 18S rDNA with that of other Ascarididae was 97.9%. Almost no intra-specific variations were detected in the 28S and 18S rDNA with the exception of a few nucleotide variations, i.e., substitution and deletion. These findings suggest that C. sinensis and Ascaris lumbricoides may be related with the biliary stone formation and development.
Aged ; Aged, 80 and over ; Animals ; Ascaridida/genetics/isolation & purification ; Ascaris lumbricoides/genetics/isolation & purification ; Base Sequence ; Clonorchis sinensis/genetics/isolation & purification ; Common Bile Duct/*parasitology ; DNA, Helminth/*genetics ; DNA, Ribosomal/genetics ; Face/parasitology ; Female ; Gallbladder/parasitology ; Gallstones/*parasitology ; Helminths/genetics/*isolation & purification ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Polymerase Chain Reaction/*methods ; RNA, Ribosomal, 18S/genetics ; RNA, Ribosomal, 28S/genetics ; Sequence Alignment

Recently, with the help of medical development which is able artificially to control respiration, circulation, nutrition and excretion, human beings may still survive in spite of brain death. We have had experience with the first case of cadaveric kidney transplantation in Korea. Because the prognosis of kidney transplantation is relatively good in comparison to the five-year survival rate of cancer patients and because postoperative rehabilitation is fair, nowadays kidney transplantation is popular. With the limitations of confluent acceptance and supplimental functioning together of the living kidney, cadaveric kidney transplantation has been preferred, although several problems still exist. We have used the conventional method to maintain the cadaver donor's life after clarification of death. As soon as brain death was confirmed on EEG monitoring, then an artificial respirator was applied to the tracheotomy site, adequate urinary excretion was maintained and infection prevented. At the same time, the HL-A(human lymphocyte-antigen) compatibility test and ABO blood matching had to be performed before anesthesia. The patient's intraoperative and postoperative course was satisfactory and he now has no problems of physical or social activity. However cadaveric kidney transplantation is in the early phase in Korea, and therefore further improvement is needed, and several problems should be solved. We hope many cadaveric kidney transplantations will be performed.
Anesthesia ; Brain Death ; Cadaver* ; Electroencephalography ; Hope ; Humans ; Kidney Transplantation* ; Kidney* ; Korea ; Methods ; Prognosis ; Rehabilitation ; Respiration ; Survival Rate ; Tracheotomy ; Ventilators, Mechanical

Patients vary markedly in their responses to d-tubocurarine chloride. Despite an attempt to diminish the variation in responses to relaxants by standardizing experimental techniques, anesthetic concentration kept constant, acid-base status kept constant, premedication omitted, dosage calculated in terms of mg/sq meter body surface, the marked variation was found to persist. The dose related neuromuscular blocking effect of d-tubocurarine chloride was investigated using a rabbit common peroneal nerve anterior tibial muscle preparation. All experimental rabbits tracheas were intubated through tracheostomy under general anesthesia with Nembutal 40 mg/kg intravenously. Reapiration was controlled by a Harvard animal respirator. The body temperature was kept at 35-37 degrees C by a thermoblanket. The degree of neuromuscular block following intravenous d-tubocurarine chloride was measured by single twitch response. The common peroneal nerve was stimulated supramaximally using a square waves of 0. 2 msec duration at a frequency of 0.1 Hz, and each stimulus was repeated once every 10 seconds. The ratio of the twitch height was calculated. The results were as follows: 1) No neuromuscular blocking effect was observed with 0.1 mg/kg of intravenous d-tubocu- rarine chloride. 2) 100% of neuromuscular blocking effect was observed with more than 1mg/kg of intravenous d-tubocurarine chloride. This is 5 to 10 times higher than the human dose. 3) Dose related prolonged neuromuscular blocking effect was observed from d-tubocurarine chloride in rabbits.
Anesthesia, General ; Animals ; Body Temperature ; Humans ; Muscle, Skeletal ; Neuromuscular Blockade* ; Pentobarbital ; Peroneal Nerve ; Premedication ; Rabbits* ; Trachea ; Tracheostomy ; Tubocurarine ; Ventilators, Mechanical

The 4,725 cattle in the enzootic region, 6 counties of southern Korea, were surveyed for besnoitiosis epidemiologically by examining the scleral conjunctiva for the cysts and the following results were obtained. The survey showed that 285 cattle, representing 6 per cent of a total of 4,725 cattle examined had S.C. cysts. Only 49 (14 per cent) of them showed clinical sclerodermatitis and the ratio between clinical and inapparent cases was 1:5.8. On age distribution of the S.C. positive cases the highest incidence(10-12 per cent) was seen in amimals that were from 5 to 8 years of age, but clinically apparent cases occurred much more in younger age of animal. In local incidence of S.C. cysts positive cases the prevalence was higher in the secluded districts such as Koheung and Sancheoung counties(9 per cent).
parasitology-besnoitiosis ; epidemiology ; prevalence rate ; scleral conjunctiva cyst

Anesthesiologists are often encountered arrhythmia during general anesthesia. Sometimes, ventricular arrhythmia may threaten patient condition to be fatal. On the other hand, anesthesiologists experience that an arrhythmia which existed pre-operatively may disappear during general anesthesia. And after anesthesia, in most of the cases, the former arrhythmia may reappear. The authors experienced one such patient who had arrhythmia pre-operatively and normal rhythm during and after general anesthesia until the 7th post-operative day. At the moment, the mechanism of disappearance of arrhythmia during general anesthesia is not clearly known, but we like to use the medical term Cardio-Anesthesia Restoration for a case who had pre-operative arrhythmia which was abolished during and after general anesthesia.
Anesthesia ; Anesthesia, General ; Arrhythmias, Cardiac ; Hand ; Humans