ABSTRACT:OBJECTIVE To explore the effect of Baicalin in vivo of the expression of NF-κB and cytokines in ulcerative coli-tis rats.METHODS The colitis model of mice were established with TNBS methord.Mice were randomly divided into control group,model group,Baicalin group,and SASP group.After continuous administration for 10 days,observed the colon gross morphological changes,the colon tissue level of TNF-α,IL-8 and IL-1 were detected using ELISA,and the protein expression of NF-κB in the colon tissues of mice were detected using Western Blot.RESULTS Compared with the model group,Baicalin(100 mg∕kg) group could significantly improve the colon gross morphological form,the Baicalin(25,50,100 mg∕kg)groups could in-hibit the secretion of inflammatory factors dose-dependently,the high dose group could reduce IL-1,IL-8 and TNF-αexpression (P <0.05),no significant difference occured between the SASP group.Compared with the model group,.Baicalin high dose group could reduce NF-kB levels,promote IkBαlevel(P <0.05) .CONCLUSION Baicalin can inhibit NF-κB activation,and thus play its anti-inflammatory effect in ulcerative colitis mice.

OBJECTIVE To develop a sensitive and selective ultra performance liquid chromatography-triple quadrupole mass spectrometer(UPLC-MS/MS) method for quantification of eight components(chrysin,baicalein,apigenin,orohylin A, wogonin,baicalin,wogonoside,oroxin A) in Schisandra chinensis Georgi.METHODS The separation was performed on a Thermo BDS Hypersil C1 8(2.1 mm×100 mm,2.3 μm) column.The mobile consisted of acetonitrile (A) and 0.1% formic acid aqueous with a gradient elution at a flow-rate of 0.25 mL/min.The detection was acquired by MRM.The column temper-ature was 40 ℃.RESULTS The linear ranges of chrysin,baicalein,apigenin,orohylin A,wogonin,baicalin,wogonoside, oroxin A were 0.975~62.5 ng/mL(r 2 =0.999 8),4.875~2 500 ng/mL(r 2 =0.999 7),0.975~62.5 ng/mL(r 2 =0.999 4), 0.097 5~12.5 ng/mL(r 2 =0.999 6),0.048 83~6.25 ng/mL(r 2 =0.999 9),4.875~2 500 ng/mL(r 2 =0.999 8),0.488 3~250 ng/mL(r 2 =0.999 9),0.488 3 ~ 250 ng/mL(r 2 = 0.999 8),respectively.The recoveries were 98.75%,96.44%, 101.13%,99.41%,102.26%,98.45%,96.75%,99.42%.CONCLUSION The proposed method enables quantification for the study of the compounds of chrysin,baicalein,apigenin,orohylin A,wogonin,baicalin,wogonoside,oroxin A in Schisan-dra chinensis Georgi.