ABSTRACT:OBJECTIVE To study the effect of isoliquiritigenin on cell proliferation,apoptosis induction,mRNA expression of endoplasmic reticulum stress related gene ATF4,DDIT3 and TNFRSF10B in NCI-H1 57 human lung cancer cell line. METHODS NCI-H1 57 cells were cultured and treated with various concentrations isoliquiritigenin.The cell proliferation was analyzed by MTT and the occurrence of cell apoptosis was examined by flow cytometry.ATF4,DDIT3 and TNFRSF10B gene mRNA expression were detected by real-time fluorescence quantitative polymerase chain reaction.RESULTS Isoliquiritigenin significantly inhibited NCI-H1 57 cell proliferation in a dose and time dependent manner.The results of flow cytometry revealed that isoliquiritigenin could induce apoptosis in NCI-H1 57 cells.Real-time quantitative RT-PCR showed that isoliquiritigenin up-regulated mRNA expression of ATF4,DDIT3 and TNFRSF10B(P <0.05 ~0.01).CONCLUSION Isoliquiritigenin can in-hibit NCI-H1 57 cell proliferation,induce cell apoptosis by activating the endoplasmic reticulum stress response.

OBJECTIVE To explore the influence of different compatibility of Shuangshen Pingfei decoction on the decocting rate of mangiferin in Anemarrhena asphodeloides ,and ingredients to research influence on the mechanism of the solution rate. METHODS To disassemble formulas on the basis of the prescription of traditional Chinese medicine containing categories of ingredients.With decocting rate of mangiferin in Anemarrhena asphodeloides Bge as indexes,analyse different compatibility influence on mangiferin decocting rate.Based on these results of disassembled formulas,the representative alkaloid 1-Deoxyno-jirimycinand amino acid in different compatibility are used to research the influence of compositions of white mulberry on the decocting rate of mangiferin in Anemarrhena asphodeloides Bge and discuss the influence of the decocting rate mechanism with Anion and cation exchange resin processing of samples.RESULTS Research findings shows that the decocting rates of man-giferin in Anemarrhena asphodeloides Bge decoction,prescription combined decoction,Anemarrhena asphodeloides Bge-sapo-nins from Chinese herbal compound,Anemarrhena asphodeloides Bge-phenolic acid from Chinese herbal compound are 97.33%,47.03%,94.08%,57.53%,34.31%,respectively;Further study shows that Morus alba L.combined with Anemarrhena asphodeloides Bge have the most obvious effect on extraction yield of mangiferin.Anemarrhena asphodeloides Bge decoting with total alkaloids of Morus alba L,DNJ,glycine,β-alanine,respectively.The decocting rate of mangiferin in each group was 1 6.64%,28.13%,40.75% and 48.56%,respectively.CONCLUSION Our results suggest that the decoc-ting rates of mangiferin are mainly influenced by Morus alba L.We also conjectured that alkaloids and amino acids of Morus alba L.form complexes with mangiferin.