Objective To analyze the imaging characteristics of patients with recurrence of acute cerebral ischemic stroke,select and predict relevant imaging factors,to guide clinical treatment and prevention.Methods Data of 30 patients with acute cerebral ischemic stroke (recurrent group)and 40 patients with primary cerebral ischemic stroke (non recurrent group)were collected.Imaging characteristics of first onset of recurrent group and non recurrent group were analyzed.Leukoaraiosis,a large area of infarction,cortical infarction,carotid artery plaque,arteriosclerosis,incomplete Willis ring,watershed infarction and other risk factors were screened,to determine the association between these risk factors and recurrent cerebral ischemic stroke.Results Recurrence of cerebral ischemic stroke was associated with leukoaraiosis,carotid artery plaque and incomplete Willis ring (P values were 0.024,0.021,0.003).There was no correlation between recurrence of cerebral ischemic stroke and a large area of infarction,cortical infarction,arteriosclerosis and watershed infarction (P values were 0.233,0.945,0.072,0.375).Conclusion Leukoaraiosis,carotid artery plaque and incomplete Willis ring may be risk factors for recurrence of cerebral ischemic stroke.Those signs can provide imaging evidence for secondary prevention of cerebral ischemic stroke.

ABSTRACT:OBJECTIVE To study the effect of isoliquiritigenin on cell proliferation,apoptosis induction,mRNA expression of endoplasmic reticulum stress related gene ATF4,DDIT3 and TNFRSF10B in NCI-H1 57 human lung cancer cell line. METHODS NCI-H1 57 cells were cultured and treated with various concentrations isoliquiritigenin.The cell proliferation was analyzed by MTT and the occurrence of cell apoptosis was examined by flow cytometry.ATF4,DDIT3 and TNFRSF10B gene mRNA expression were detected by real-time fluorescence quantitative polymerase chain reaction.RESULTS Isoliquiritigenin significantly inhibited NCI-H1 57 cell proliferation in a dose and time dependent manner.The results of flow cytometry revealed that isoliquiritigenin could induce apoptosis in NCI-H1 57 cells.Real-time quantitative RT-PCR showed that isoliquiritigenin up-regulated mRNA expression of ATF4,DDIT3 and TNFRSF10B(P <0.05 ~0.01).CONCLUSION Isoliquiritigenin can in-hibit NCI-H1 57 cell proliferation,induce cell apoptosis by activating the endoplasmic reticulum stress response.

ABSTRACT:OBJECTIVE To explore the effect of Baicalin in vivo of the expression of NF-κB and cytokines in ulcerative coli-tis rats.METHODS The colitis model of mice were established with TNBS methord.Mice were randomly divided into control group,model group,Baicalin group,and SASP group.After continuous administration for 10 days,observed the colon gross morphological changes,the colon tissue level of TNF-α,IL-8 and IL-1 were detected using ELISA,and the protein expression of NF-κB in the colon tissues of mice were detected using Western Blot.RESULTS Compared with the model group,Baicalin(100 mg∕kg) group could significantly improve the colon gross morphological form,the Baicalin(25,50,100 mg∕kg)groups could in-hibit the secretion of inflammatory factors dose-dependently,the high dose group could reduce IL-1,IL-8 and TNF-αexpression (P <0.05),no significant difference occured between the SASP group.Compared with the model group,.Baicalin high dose group could reduce NF-kB levels,promote IkBαlevel(P <0.05) .CONCLUSION Baicalin can inhibit NF-κB activation,and thus play its anti-inflammatory effect in ulcerative colitis mice.

OBJECTIVE To develop a sensitive and selective ultra performance liquid chromatography-triple quadrupole mass spectrometer(UPLC-MS/MS) method for quantification of eight components(chrysin,baicalein,apigenin,orohylin A, wogonin,baicalin,wogonoside,oroxin A) in Schisandra chinensis Georgi.METHODS The separation was performed on a Thermo BDS Hypersil C1 8(2.1 mm×100 mm,2.3 μm) column.The mobile consisted of acetonitrile (A) and 0.1% formic acid aqueous with a gradient elution at a flow-rate of 0.25 mL/min.The detection was acquired by MRM.The column temper-ature was 40 ℃.RESULTS The linear ranges of chrysin,baicalein,apigenin,orohylin A,wogonin,baicalin,wogonoside, oroxin A were 0.975~62.5 ng/mL(r 2 =0.999 8),4.875~2 500 ng/mL(r 2 =0.999 7),0.975~62.5 ng/mL(r 2 =0.999 4), 0.097 5~12.5 ng/mL(r 2 =0.999 6),0.048 83~6.25 ng/mL(r 2 =0.999 9),4.875~2 500 ng/mL(r 2 =0.999 8),0.488 3~250 ng/mL(r 2 =0.999 9),0.488 3 ~ 250 ng/mL(r 2 = 0.999 8),respectively.The recoveries were 98.75%,96.44%, 101.13%,99.41%,102.26%,98.45%,96.75%,99.42%.CONCLUSION The proposed method enables quantification for the study of the compounds of chrysin,baicalein,apigenin,orohylin A,wogonin,baicalin,wogonoside,oroxin A in Schisan-dra chinensis Georgi.