OBJECTIVETo investigate the chemical constituents from the leaves of Vaccinium bracteatum.

METHODMany column chromatographic techniques were used for the isolation and separation of chemical constituents. Their structures were elucidated on the basis of spectral analysis and chemical evidences.

RESULTTwelve compounds were isolated from the plant, and they were identified as chrysoeriol (1), scopoletin (2), trans-p-hydroxycinnamic acid (3), trans-p-hydroxycinnamic acid ethyl ester (4), cafeic acid ethyl ester (5), beta-sitosterol (6), iuteolin (7), quercetin (8), esculetin (9), cafeic acid (10), isolariciresinol-9-O-beta-D-xyloside (11), 10-O-trans-p-coumaroylsandoside (12).

CONCLUSIONCompounds 4, 5, 11, 12 were isolated from the genus Vaccinium for the first time, and compounds 1, 2, 9, 10 were isolated from this plant for the first time.

Coumaric Acids ; chemistry ; Flavones ; Flavonoids ; chemistry ; Furans ; chemistry ; Glycosides ; chemistry ; Lignin ; chemistry ; Molecular Structure ; Plant Leaves ; chemistry ; Scopoletin ; chemistry ; Sitosterols ; chemistry ; Umbelliferones ; chemistry ; Vaccinium ; chemistry

OBJECTIVETo investigate the chemical constituents of Saussurea laniceps.

METHODThe ethanol extract of S. laniceps was separated by means of silica gel chromatography. The compounds isolated from the plant were identified by their spectral evidence.

RESULTFifteen compounds were isolated and identified as beta-stiosterol (1), umbelliferone (2), 4-hydroxyacetophenone (3), scopoletin (4), isoscopoletin (5), xuelianlactone (6), methyl 3-(2', 4'-dihydroxyphenyl) propanoate (7), apigenin (8), neoechinulin A (9), daucosterol (10), scopolin (11), xuelianlactone 8-O-beta-D-glcuoside (12), apigenin 7-glcuoside (13), apigenin 7-lutinoside (14) and syringin (15).

CONCLUSIONCompounds 5-15 were isolated from S. laniceps, and among them, 7 and 9 were isolated from genus Saussurea for the first time.

Apigenin ; chemistry ; Coumarins ; chemistry ; Glucosides ; chemistry ; Magnetic Resonance Spectroscopy ; Phenylpropionates ; chemistry ; Phorbols ; chemistry ; Saussurea ; chemistry ; Scopoletin ; analogs & derivatives ; chemistry ; Sesquiterpenes ; chemistry ; Umbelliferones ; chemistry

OBJECTIVETo investigate the chemical constituents from the branch of Fraxinus sieboldiana, and evaluate their antioxidative activity.

METHODThe chemical constituents were isolated and purified by chromatographic techniques over silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC. Structures of the compounds were identified by spectroscopic methods. The antioxidant activity was evaluated by Fe(+2)-cystine induced rat liver microsomal lipid peroxidation.

RESULTEight coumarins were obtained and their structures were elucidated as esculin (1) , esculetin (2), fraxin (3), fraxetin (4), 6, 7-di-O-beta-D-glucopyranosylesculetin (5), scopoletin (6), cleomiscosin D (7) and cleomiscosin B (8). At a concentration of 10(-6) mol x L(-1), compound 4 showed antioxidative activity inhibiting Fe(+2)-cystine induced rat liver microsomal lipid peroxidation with inhibitory rate of 60%.

CONCLUSIONCompounds 5, 7 and 8 were obtained from the genus Fraxinus for the first time. Compound 4 showed remarkable antioxidative activity, which was higher than that of VE (35%).

Animals ; Antioxidants ; chemistry ; pharmacology ; Coumarins ; chemistry ; pharmacology ; Fraxinus ; chemistry ; Lipid Peroxidation ; drug effects ; Magnetic Resonance Spectroscopy ; Microsomes, Liver ; drug effects ; metabolism ; Rats ; Scopoletin ; chemistry ; pharmacology ; Spectrometry, Mass, Electrospray Ionization ; Umbelliferones ; chemistry ; pharmacology

To study chemical constituents of Eupatorium lindleyanum. Ethyl acetate extractive fractions were separated with silica gel and Sephadex LH-20 by column chromatography, and their structures were identified on the basis of spectroscopic analysis and chemical evidence. Sixteen compounds were separated and identified as scopoletin (1), 6, 7-dimethylesculetin (2), nepetin (3), eupatrin (4), luteolin (5), isoquerecitrin (6), jaceosidin (7), quceritin (8), kaempferol (9), rutin (10), cirsiliol (11), taraxasterylacetate (12), pseudotaraxasteryl acetate (13), pseudotaraxasterol (14), butanoic acid (15) and n-hexadecanoic acid (16). Of them, compounds 1-6 and 11, 13 and 15 were separated from this plant for the first time.
Acetates ; chemistry ; Butyric Acid ; chemistry ; Eupatorium ; chemistry ; Flavones ; chemistry ; Flavonoids ; chemistry ; Kaempferols ; chemistry ; Luteolin ; chemistry ; Palmitic Acid ; chemistry ; Quercetin ; analogs & derivatives ; chemistry ; Rutin ; chemistry ; Scopoletin ; chemistry ; Sterols ; chemistry ; Triterpenes ; chemistry ; Umbelliferones ; chemistry

OBJECTIVETo study the chemical constituents of the roots of Ferula sinkiangensis.

METHODCompounds were isolated by repeated chromatography on silica gel. Their structures were elucidated by chemical and spectroscopic methods.

RESULTSeven compounds were identified as fekrynol (1), fekolone (2), farnesiferol B (3), isosamarcandin (4), episamarcandin (5), farnesiferol C (6), umbelliferone (7).

CONCLUSIONAll the compounds were obtained from this plant for the first time.

Ferula ; chemistry ; Molecular Structure ; Plants, Medicinal ; chemistry ; Sesquiterpenes ; chemistry ; isolation & purification ; Umbelliferones ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents of Artemisia rupestris.

METHODThe chemical constituents were isolated by column chromatography on silical gel and sephadex LH - 20. Their structures were elucidated on the basis of spectral analysis.

RESULT8 compounds have isolated from this plant, and the structures of them have identified as rupestonic acid (1), chrysosplenetin B (2), artemetin (3), herniarin (4), isokaempferide (5), vanillic acid (6), kaempferol 3, 3', 4'-trimethyl ether (7) and ermanine (8).

CONCLUSIONCompounds 2-8 have been isolated from this plant for the first time.

Artemisia ; chemistry ; Flavonoids ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Umbelliferones ; chemistry ; isolation & purification

OBJECTIVETo screen the antimalarial compounds of daphnetin derivatives against Plasmodium falciparum in vitro.

METHODPlasmodium faciparum (FCC1) was cultured in vitro by a modified method of Trager and Jensen. Antimalarial compounds were screened by microscopy-based assay and microfluorimetric method.

RESULTSDA79 and DA78 showed potent antimalarial activity against Plasmodium falciparum cultured in vitro.

CONCLUSIONThough the relationship between the structures of daphnetin derivatives and their antimalarial activities has not been clarified yet, this study may provide a new direction for discovery of more potential antimalarial compounds.

Animals ; Antimalarials ; chemistry ; pharmacokinetics ; pharmacology ; Plasmodium falciparum ; drug effects ; Umbelliferones ; chemistry ; pharmacokinetics ; pharmacology

The root of Peucedanum harry-smithii var. subglabrum was extracted with methanol, then separated with solvents at different polarity into four fractions: aqueous (H2O), ethyl acetate (AcOEt), chloroform (CHCl3) and petroleum ether (DAB-6). From AcOEt psoralen, bargapten, xanthotoxin, marmesin, umbelliferone, scopoletin, (+/-) peuformosin, Pd-I b, (+/-) selinidin, praeruptorin D were isolated by column chromatography on silica gel, using petroleum ether/ethyl acetate as eluent. The structures of the coumarins were identified by 1H-NMR and 13C-NMR.
Apiaceae ; chemistry ; Coumarins ; chemistry ; isolation & purification ; Magnetic Resonance Spectroscopy ; methods ; Plant Roots ; chemistry ; Umbelliferones ; chemistry

Recently, loss of endogenous glutathione during N-methyl-D-aspartate (NMDA) receptor-mediated excitotoxic injury, and the resultant overproduction of reactive oxygen species (ROS) through an arachidonic acid cascade process in brain, have been implicated in neuronal damage in various neurodegenerative diseases. Glutathione depletion induced by L-buthionine-(S,R)-sulfoximine (BSO), an inhibitor of glutathione synthesis, is known to cause arachidonic acid-mediated excitotoxicity in primary mixed cortical cultures. The aim of this study was to investigate whether esculetin (6,7-dihydroxycoumarin), an inhibitor of lipoxygenase, protects against neurotoxicity induced by NMDA or BSO. We observed that neurotoxicity induced by NMDA but not kainic acid was attenuated by esculetin. At the same concentration (100 microM), esculetin attenuated the 45Ca2+ uptake elevation induced by NMDA. Free radical-mediated neuronal injury induced by H2O2 and xanthine/xanthine oxidase was concentration-dependently blocked by esculetin. Esculetin (1-30 microM) dose-dependently inhibited BSO-induced neuronal injury. In addition, arachidonate-induced neurotoxicity was completely blocked by esculetin. BSO also reduced glutathione peroxidase (GPx) activity, but did not change glutathione reductase (GR) activity 24 h after treatment. Esculetin dose-dependently increased GR activity, but did not alter GPx activity. These findings suggest that esculetin can contribute to the rescue of neuronal cells from NMDA neurotoxicity and that this protective effect occurs partly through NMDA receptor modulation and the sparing of glutathione depletion.
Arachidonic Acid ; Brain ; Glutathione ; Glutathione Reductase ; Kainic Acid ; Lipoxygenase ; N-Methylaspartate ; Neurodegenerative Diseases ; Neurons ; Oxidoreductases ; Peroxidase ; Reactive Oxygen Species ; Umbelliferones

This study is aimed to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of skimmin, scopolin and umbelliferone in Saussurea hieracioides. Samples were analyzed on a Wondasil C18-WR column (4.6 mm x 250 mm, 5 microm) with methanol (A) and water containing 0.1% phosphate (B) as mobile phases for gradient elution at a flow rate of 1.0 mL x min(-1). The detection wavelength and column temperature were set at 325 nm and 35 degrees C, respectively, and the sample size was 10 microL. The results showed that skimmin, scopolin and umbelliferone were simultaneously achieved within 40 min under the above conditions. A good linearity was observed in the range of 0.18-5.6 microg (r = 1.000 0), 0.060-1.8 microg (r = 0.999 9), 0.032-0.97 microg (r = 0.999 8) for skimmin, scopolin and umbelliferone, respectively, with the average recoveries of 99.16% (RSD = 0.41%), 100.3% (RSD = 0.79%), 102.2% (RSD = 0.87%). The method is simple, accurate and reproducible and can be used for the quality control of S. hieracioides.
Chromatography, High Pressure Liquid ; Coumarins ; analysis ; Glucosides ; analysis ; Medicine, Tibetan Traditional ; Reproducibility of Results ; Saussurea ; chemistry ; Umbelliferones ; analysis