OBJECTIVE To observe the inhibitory effects of Liuwei Dihuang Pills(LWDHP) on macrophages activation in CCl4-induced liver fibrosis in mice.METHODS C57BL/6 mice were induced liver fibrosis by CCl4 exposure and administered with LWDHP for 6 weeks simultaneously.Liver tissue was investigated by HE and Sirius red staining.α-SMA was analyzed by immunofluorescence,qPCR and Western blot.Liver macrophages were observed by immunohistochemistry of CD68.The pro-inflammatory cytokines and chemokine such as TNF-α,IL-1β,CXCR3 and MCP1 were detected by qPCR or Western blot analysis.RESULTS After 6 weeks of CCl4 administration,the expression ofα-SMA significantly increased,and LWDHP po-tently inhibited the α-SMA expression.Immunohistochemistry showed that the expression of CD68 was very weak in normal group,CD68 was distributed mainly between fibrotic septa with strong positive expression in CCl4 model group;Real-time quantitative PCR showed that TNF-α,IL-1β,MCP1 and CXCR3 expression significantly increased in model group compared with normal group.Compared with the model group,LWDHP significantly reduced the expression of CD68,inflammatory fac-tors and chemotactic factors.CONCLUSION LWDHP shows a potent inhibition of macrophage activation in CCl4-induced liv-er fibrosis.