1.2-Hexyl-4-Pentylenic Acid (HPTA) Stimulates the Radiotherapy-induced Abscopal Effect on Distal Tumor through Polarization of Tumor-associated Macrophages.
Wen Hua DUAN ; Li Ya JIN ; Zu Chao CAI ; David LIM ; Zhi Hui FENG
Biomedical and Environmental Sciences 2021;34(9):693-704
Objective:
The aim of this study was to explore the effects of 2-hexyl-4-pentylenic acid (HPTA) in combination with radiotherapy (RT) on distant unirradiated breast tumors.
Methods:
Using a rat model of chemical carcinogen (7,12-dimethylbenz[a]anthracene,DMBA)-induced breast cancer, tumor volume was monitored and treatment response was evaluated by performing HE staining, immunohistochemistry, immunofluorescence, qRT-PCR, and western blot analyses.
Results:
The results demonstrated that HPTA in combination with RT significantly delayed the growth of distant, unirradiated breast tumors. The mechanism of action included tumor-associated macrophage (TAM) infiltration into distant tumor tissues, M1 polarization, and inhibition of tumor angiogenesis by IFN-γ.
Conclusion
The results suggest that the combination of HPTA with RT has an abscopal effect on distant tumors
Animals
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Antineoplastic Agents/therapeutic use*
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Cell Proliferation/radiation effects*
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Combined Modality Therapy
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Cytokines/immunology*
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Fatty Acids, Unsaturated/therapeutic use*
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Female
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Mammary Neoplasms, Experimental/radiotherapy*
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Rats
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Tumor-Associated Macrophages/radiation effects*
Result Analysis
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