1.Performance of the SD Bioline TB Ag MPT64 Rapid test for quick confirmation of Mycobacterium bovis isolates from animals.
Hyeon Seop BYEON ; Mi Jung JI ; Shin Seok KANG ; Sang Woo KIM ; Seung Cheol KIM ; Song Yong PARK ; Geehyuk KIM ; Jiro KIM ; Jang Eun CHO ; Bok Kyung KU ; Jae Myung KIM ; Bo Young JEON
Journal of Veterinary Science 2015;16(1):31-35
Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. Mycobacterial culture is the gold standard for diagnosing bovine tuberculosis, but this technique is laborious and time-consuming. In the present study, performance of the SD Bioline TB Ag MPT4 Rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains and M. bovis field isolates collected from animals. The SD MPT64 Rapid test produced positive results for 95.5% (63/66) of the M. bovis isolates from cattle and 97.9% (46/47) of the isolates from deer. Additionally, the test had a sensitivity of 96.5% (95% CI, 91.2-99.0), specificity of 100% (95% CI, 96.7-100.0), positive predictive value of 100% (95% CI, 96.7-100.0), and negative predictive value of 92.9% (95% CI, 82.7-98.0) for M. bovis isolates. In conclusion, the SD MPT64 Rapid test is simple to use and may be useful for quickly confirming the presence of M. bovis in animals.
Animals
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Cattle
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Cattle Diseases/*diagnosis/microbiology
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*Deer
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Immunochromatography/methods/*veterinary
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Mycobacterium bovis/classification/*isolation & purification
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Sensitivity and Specificity
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Tuberculosis/diagnosis/microbiology/*veterinary
2.Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay.
Sungmo JE ; Un Chang YEO ; Taeksun SONG ; Ki Cheol KIM ; Sung Yun PARK ; Man Jung KIM ; Sang Nae CHO
Journal of Veterinary Science 2014;15(2):259-265
The interferon-gamma (IFN-gamma) assay is employed as a complementary diagnostic test for bovine tuberculosis (BTB) in many countries. To simplify this assay, we established a 96-well plate format using the ESAT-6 and CFP-10 antigens and then employed it to determine the extent of Mycobacterium (M.) bovis infection in dairy herds with a history of BTB outbreaks in a country where only selective culling is practiced. The sensitivity and specificity of this IFN-gamma assay were 85.9% and 100%, respectively, based on comparison with the conventional single intradermal tuberculin test (SIDT). The IFN-gamma assay was also positive in 30.4% and 36.8% of SIDT-negative animals from herds with recent and remote BTB outbreaks, respectively. Of 14 SIDT-negative, IFN-gamma positive cattle, five (35.7%) were culture positive and an additional six were positive based on a polymerase chain reaction-based test for M. bovis. Therefore, the IFN-gamma assay has the potential to serve as a specific and sensitive test for M. bovis infection in dairy cattle. Further, the results indicated that a substantial portion of SIDT-negative animals in herds with previous BTB outbreaks were actually infected with M. bovis. Accordingly, the present selective-culling strategy may require modifications to include this more sensitive assay.
Animals
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Antigens, Bacterial/*diagnostic use
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Bacterial Proteins/diagnostic use
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Cattle
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Female
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Interferon-gamma Release Tests/*veterinary
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Mycobacterium bovis/*isolation & purification
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Polymerase Chain Reaction/veterinary
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Republic of Korea/epidemiology
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Tuberculosis, Bovine/*diagnosis/*epidemiology/microbiology