1.Monocyte chemotactic protein-1 gene polymorphism and monocyte chemotactic protein-1 expression in Chongqing Han children with tuberculosis.
Zhen-e XU ; Yuan-yuan XIE ; Jun-hua CHEN ; Lin-lin XING ; Ai-hua ZHANG ; Ben-xiu LI ; Chao-min ZHU
Chinese Journal of Pediatrics 2009;47(3):200-203
OBJECTIVEThe aims of this study were to evaluate whether the presence of -2518A/G polymorphism in the distal regulatory region of the monocyte chemotactic protein-1 (MCP-1) was associated with tuberculosis (TB) in Chongqing Han population and to find whether it has a significant impact on the pediatric patient.
METHODOne hundred children [ < or = 15 years old, mean age (7.3+/-4.6) years, 53 male, 47 female] and one hundred adults [51 male, 49 female, age (44.6+/-13.5) years with TB] and 200 healthy controls of comparable age were screened for genotype by PCR-sequence-specific primer (SSP) method. MCP-1 levels in the sera were detected by ELISA.
RESULT(1) TB patients and controls showed different single nucleotide polymorphism (SNP) distribution patterns (58%, 36%). MCP-1 alleles -2518G was associated with increased TB susceptibility (P<0.01). (2) The -2518 GG genotypes was associated with increased TB susceptibility (32% in TB patients and 13% in non-TB controls respectively, P<0.01). (3) The odds of developing TB in genotypes GG were higher than those in homozygous AA, and the risk was higher in children than in adult (7.0-fold in children and 5.1-fold in adults, respectively). (4) Cases of homozygous GG had the highest plasma levels of MCP-1, which increased the likelihood of developing TB. Furthermore, higher levels were observed in children than in adults.
CONCLUSIONThese findings suggest that persons bearing the MCP-1 genotype GG produce high concentrations of MCP-1, which increases the risk of active TB infection in Chongqing Han people. These findings are more significant in child patients than in adult patients with TB.
Adult ; Alleles ; Asian Continental Ancestry Group ; genetics ; Case-Control Studies ; Chemokine CCL2 ; blood ; genetics ; Child ; Child, Preschool ; DNA Primers ; Female ; Genetic Predisposition to Disease ; Genotype ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Tuberculosis ; ethnology ; genetics ; metabolism
2.Study on immunogenicity elicited by a recombinant vaccine of rBCG-Rv3133c to fight against dormancy Mycobacterium tuberculosis.
Yihao DENG ; Hongyun HE ; Bensi ZHANG
Journal of Biomedical Engineering 2013;30(4):817-821
To obtain a vaccine to defend from dormancy Mycobacterium tuberculosis, we constructed the recombinant Bacilli Calmette-Guérin (BCG) vaccine with Rv3133c encoding dormancy-correlated transcriptional regulatory protein DosR in Mycobacterium tuberculosis as a target gene, and evaluated its immunogenicity in BALB/c mice. In this study, we constructed the recombinant plasmids of rpMV361-Rv3133c using gene colon technology. We then transformed BCG strains with above-mentioned plasmids to obtain recombinant vaccine of rBCG-Rv3133c. We used the rBCG strains successfully constructed to vaccinate in BALB/c mice. 30d and 180d after immunization, the specific antibody titers were determined to investigate humoral responses induced by recombinant vaccine. We detected changes of splenocyte subsets of CD4+T, CD8+ T cells and cytokine of IFN-gamma secreted by splenocytes for evaluation of cellular immune responses. The results showed that the rBCG-Rv3133c was able to induce higher levels of antibody titer, stronger proliferative responses and higher IFN-gamma production comparing with BCG vaccine. The results also suggested that this recombinant vaccine was a more efficacious tuberculosis vaccine for further study.
Animals
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Antibodies, Bacterial
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blood
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Antigens, Bacterial
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genetics
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immunology
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BCG Vaccine
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immunology
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Bacterial Proteins
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genetics
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immunology
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Escherichia coli
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genetics
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metabolism
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Interferon-gamma
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immunology
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Mice
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Mice, Inbred BALB C
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Mycobacterium tuberculosis
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immunology
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Protein Kinases
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genetics
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immunology
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Recombinant Proteins
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immunology
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T-Lymphocyte Subsets
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immunology
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Tuberculosis
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prevention & control
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Vaccination
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Vaccines, Synthetic
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immunology
3.Proteomic Profiling of Serum from Patients with Tuberculosis.
Sang Hoon SONG ; Minje HAN ; Yang Seon CHOI ; Ki Soon DAN ; Man Gil YANG ; Junghan SONG ; Sung Sup PARK ; Jae Ho LEE
Annals of Laboratory Medicine 2014;34(5):345-353
BACKGROUND: Effective treatment and monitoring of tuberculosis (TB) requires biomarkers that can be easily evaluated in blood samples. The aim of this study was to analyze the serum proteome of patients with TB and to identify protein biomarkers for TB. METHODS: Serum samples from 26 TB patients and 31 controls were analyzed by using nano-flow ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in data-independent mode, and protein and peptide amounts were calculated by using a label-free quantitative approach. The generated data were analyzed by using principal component analysis and partial least squares discriminant analysis, a multivariate statistical method. RESULTS: Of more than 500 proteins identified, alpha-1-antitrypsin was the most discriminative, which was 4.4 times higher in TB patients than in controls. Peptides from alpha-1-antitrypsin and antithrombin III increased in TB patients and showed a high variable importance in the projection scores and coefficient in partial least square discriminant analysis. CONCLUSIONS: Sera from patients with TB had higher alpha-1-antitrypsin levels than sera from control participants. Alpha-1-antitrypsin levels may aid in the diagnosis of TB.
Adult
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Aged
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Antithrombin III/analysis
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Biological Markers/blood
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Chromatography, High Pressure Liquid
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Discriminant Analysis
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Female
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Humans
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Male
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Middle Aged
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Multivariate Analysis
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Proteome/*analysis
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*Proteomics
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Tuberculosis/*blood/genetics/metabolism
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alpha 1-Antitrypsin/analysis