1.Preparation and immunogenicity of silk fibroin/chitosan microspheres for DNA vaccine delivery against infectious bursal disease virus.
Yan LIU ; Zhiqiang LV ; Cun ZHANG ; Xingrong ZHU ; Tuanyuan SHI ; Shi ZHONG ; Zhiqi MENG
Chinese Journal of Biotechnology 2014;30(3):393-403
To evaluate the immunities of biodegradable microsphere as a release delivery system for DNA vaccine against Infectious Bursal Disease Virus, in our study, silk fibroin/chitosan microsphere adjuvant was prepared with a precipitation/coacervation method. Both glutaraldehyde and Na2SO4 solution were used in cross-linking. No immune chicken were intramuscularly inoculated at 14 day-old and boosted 2 weeks later. The results show that glutaraldehyde destroyed the DNA activity of the vaccine whereas Na2SO4 solution did not. Factors of the chitosan concentration 0.5% (pH 5.0), silk fibroin concentration 0.6%, plasmid DNA (500 microg/mL) dissolved in 2% Na2SO4 solution were optimized to produce microsphere, with a loading capacity of 89.14%. The average particle size of SF-CS/pCI-VP2/4/3 microsphere is 1.98 microm, and it can protect the loading DNA vaccine from DNase I digestion. Data from anti IBDV ELISA antibodies in the serum show that immunization activity of the microsphere groups were generally higher than plasmid vaccine group (P < 0.05), and the SF/CS compound microspheres group was better than that of sole CS microsphere group. The developed SF/CS microspheres are a very promising vaccine delivery system.
Adjuvants, Immunologic
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chemistry
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Animals
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Birnaviridae Infections
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prevention & control
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veterinary
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Chickens
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Chitosan
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chemistry
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Fibroins
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chemistry
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Infectious bursal disease virus
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Microspheres
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Plasmids
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Poultry Diseases
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prevention & control
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Vaccines, DNA
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chemistry
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Viral Vaccines
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chemistry