Objective We aimed to investigate the role of McMaster model of family therapy in improving family function of patients with advanced hepatocellular carcinoma (HCC).Methods Patients who had advanced HCC and received transarterial embolization (TAE) or transarterial chemoembolization (TACE) from Department of Hepatobiliary Ⅰ,Eastern Hepatobiliary Surgery Hospital between October 1,2012 and June 25,2013 were randomly divided into two groups:the experimental group (51 patients) and the control group (49 patients).The control group received routine family support education.The experimental group not only executed routine family support education,but also was given McMaster model of family therapy according to evaluation results of family assessment device (FAD) on the second and third day of hospitalization.The status of family function of all patients were assessed by FAD on the fnrst day of hospitalization and fourth week after therapy.We compared the status of family function between the two groups.Results In age,gender,educational level,place of residence,occupation,family economic status,medical payment,liver or kidney function,HBV infection,cirrhosis and tumor burden,no statistical differences were found between the experimental group and the control group patients before TAE or TACE.Two groups were dysfunction in communication,roles,affective responsiveness,affective involvement,behavior control,and general function in addition to problem solving before TAE or TACE.No statistical differences were found between two groups.After the therapy,compared with the control group,those patients in the experimental group had a lower level scoring in communication,roles,affective responsiveness,affective involvement,behavior control,and general function in addition to problem solving on the fourth week after the therapy,t value was-2.544,-3.767,-3.904,-2.848,-4.950 and-4.953,the difference had statistical significance.Conclusions McMaster model of family therapy may help to improve family function of patients with advanced HCC.

To explore the diagnostic value of 75 commonly used clinical laboratory markers for differentiation of traditional Chinese medicine syndromes such as liver and gallbladder damp-heat and liver depression and spleen deficiency in patients with chronic hepatitis B.

Objective:To investigate the attitude to death of oncology nurses and analysis its influencing factors,so as to provide scientific references and basis for care of the dying patients.Methods:A convenience sampling method was used to 309 nurses from Eastern Hepatobiliary Surgery Hospital,the Third Affiliated Hospital of Naval Military Medical University.The survey was conducted by filling in the electronic questionnaire which powered by sojump.com. The questionnaire was composed with a demographic survey and Chinese version Death Attitude Profile-Revised.Results:In the five dimensions of Death Attitude Profile-Revised,the highest factor score was in Approach Acceptance(3.56±0.74),the second was in Escape Acceptance(3.54±0.85) and the lowest was in Natural Acceptance(2.30±0.53).There was a statistically significant difference in the number of years of work in Nautral Acceptance( F value was 3.400, P<0.05).Parents with or without religious beliefs had statistically significant differences in Death of Avoidance( t value was 6.207, P<0.05). Consciously aware that the current physical condition was statistically different from Approach Acceptance and Escape Acceptance( F value was 3.494, 2.606, all P<0.05).Consciously the current psychological status was statistically different in Death Fear and Approach Acceptance and Escape Acceptance( F value was 3.381, 3.125, 3.911, all P<0.05).The situation of talking death in the family was statistically different between Death Fear and Death of Avoidance ( F value was 2.899, 2.762, all P<0.05).There was a statistically significant difference in Death Fear and Death of Avoidance and Death Education ( F value was 3.581, 5.336, all P<0.05). Conclusions:The attitude to death in 309 oncology nurses is relatively passive.The main influencing factors are work experience and parents with or without religious beliefs and the situation of talking death in the family physiology and psychology conditions,whether or not receive death education.

BACKGROUND: Paeonol is an active ingredient of traditional orthopedic drugs, exhibiting pain easing, detumescence and promotion of bone healing. The brushite calcium phosphate cement possesses good biocompatibility, which can be completely degraded in the body. A large number of studies have addressed the modification of brushite calcium phosphate cement to make it more suitable for clinical applications. OBJECTIVE: To study the setting time, syringeability, compressive strength, drug delivery ability, antibacterial property and cell affinity of brushite calcium phosphate cement with the addition of paeonol. METHODS: The tricalcium phosphate was synthesized by calcium nitrate and diammonium hydrogen phosphate, followed by being calcined into β-tricalcium phosphate at 1 000 ℃. Chitosan was dissolved in citric acid solution to prepare the liquid phase. The composite bone cement was prepared by mixing paeonol, β-tricalcium phosphate and monocalcium phosphate with the liquid phase. The setting time, syringeability, phase composition, anti-collapsibility, compressive strength, degradation property, drug delivery ability, antibacterial property and cell affinity of the composite bone cement in vitro were evaluated. RESULTS AND CONCLUSION: The setting time of bone cement was 12-17 minutes, which was prolonged with the increase of paeonol release. Moreover, the loaded paeonol showed no significant effect on the phase composition, syringeability, anti-collapsibility, and compressive strength of the compound. However, the degradation rate and drug release content were significantly enhanced with the increase of paeonol release.The inhibition zone experiments showed that the paeonol loaded cement inhibited the growth of Escherichia coli, but was not sensitive to Staphylococcus aureus.HepG2 cells could adhere and proliferate on the material surface after 3 days co-culture,with clear cell pseudopodia arising from the cell surface under the scanning electron microscope.

OBJECTIVETo investigate the carrier rate of thalasaemia among the children of 10 minority ethnic groups in 3 border states (Xishuangbanna, Dehong and Nujiang) of Yunnan Province.

METHODSA total of 6562 samples of children under 10 years old were analyzed by blood cell automatic analysis and hemoglobin electrophoresis.

RESULTSThe overall carrier frequency of thalasaemia was highest (46.2%) in Dehong, and lowest (30.6%) in Nujiang. The carrier frequency of beta-thalasaemia was the highest (40.6%) in Achang, and lowest (2.5%) in Dulong. The carrier frequency of alpha-thalasaemia was the highest (22.1%) in Dai from Xishuangbanna, followed by Dulong (19.1%).

CONCLUSIONThalasaemia carrier rates were high among the children of 10 minority ethnic groups in Yunnan. There were regional differences in their incidences. The results provide a valuable basis for thalasaemia prevention in Yunnan minorities in the three border states.

Child ; Child, Preschool ; China ; epidemiology ; Hemoglobins, Abnormal ; genetics ; Humans ; Infant ; Infant, Newborn ; Minority Groups ; Prevalence ; Thalassemia ; ethnology ; genetics ; alpha-Thalassemia ; epidemiology ; genetics ; beta-Thalassemia ; epidemiology ; genetics

OBJECTIVETo investigate the level and distribution characteristics of anemia of the minority ethnic group children in Yunnan.

METHODThe cases with anemia were surveyed from 13 336 samples of 15 minority ethnic groups and Han children in Yunnan by Taking the method of random cluster sampling.

RESULTThe prevalence of anemia among the children under 7 years of age of 15 ethnic groups of minority in Yunnan was 13.6%. There are differences among the different ethnic groups (χ(2) = 716.33, P < 0.01), the highest was 26.6% in Jingpo, the lowest was 3.5% in Bai. There were differences among the different regions, the prevalence of anemia was high in the border regions City, the highest was 23.8% in Dehong; the prevalence of anemia was low in inland cities, the lowest was 2.7% in Fugong, and was higher in border areas. The prevalence of anemia was higher in boys (13.6%) than in girls (12.1%). There were differences among the different age in the different ethnic groups (6 months to 1 years old: χ(2) = 70.52, P < 0.01; 1 - 2 years old:χ(2) = 185.86, P < 0.01; 2 - 5 years old: χ(2) = 296.12, P < 0.01; 5 - 6 years old:χ(2) = 107.11, P < 0.01; 6 - 7 years old:χ(2) = 185.02, P < 0.01), the highest was 59.0% of Deang in 1 to 2 years old children. The trend of change was that the highest prevalence was seen in 6 months to 1 year old children, the prevalence gradually declined among older children, but rose again in children 6 years of age or older.

CONCLUSIONThe prevalence of anemia was 13.6% among the children of 15 ethnic minority under 7 years of age in Yunnan. There were differences among different ethnic groups of minority in different prefectures. There were differences among different ethnic groups of different age groups, but it was highest in 6 months to 1 year old children, it declined among older children, and rose in children 6 years of age or older. The prevalence of anemia was related to the ethnic and geographic factors.

Age Distribution ; Altitude ; Anemia ; diagnosis ; epidemiology ; ethnology ; Biomarkers ; analysis ; Child ; Child, Preschool ; China ; epidemiology ; ethnology ; Ethnic Groups ; Female ; Hemoglobins ; analysis ; Humans ; Infant ; Infant, Newborn ; Male ; Minority Groups ; statistics & numerical data ; Prevalence ; Sampling Studies ; Sex Distribution

Objective To investigate the prevalence rate of thalassemia among children of 0-7 years old,from six ethnic groups in Xishuangbanna and Dehong.Yunnan province.Methods 4973 blood samples from children under 7 years old were automatically undergone blood cell count,red cell osmotic fragility and hemoglobin electrophoresis testings.Results The incidence rates of thalassaemia,β-thalassemia was 37.4%,and α-thalassaemia were 22.6%and 14.7% respectively.The thalassaemia incidence rates were significantly different among age groups but not in gender. The incidence of α-thalassaemia was decreasing along with the increase of age.while the incidence of β-thalassaemia was increasing along with the increase of age.Xishuangbanna had the higher incidence than in Dehong and the differences were significant between counties.The incidence of thalassemia of Mengla ranked the first(52.2%)in Xishuangbanna,The difierences between different regions and different nationalities were significant,with β-thalassemia of Achang ranked the first(40.6%),The incidence of α-thalassemia among Han ranked the first as 45.5% while α-thalassaemia and β-thalassemia were different in regions.α-thalassaemia and β-thalassemia were significantly different between different ethnic people in the same regions.Multiple factor analysis showed that region seemed to be a risk factor and the mother's ethnicity was a protective factor and dependent variable on thalassaemia.Conclusion The incidence of thalassaemia in Yunnan Xishuangbanna and Dehong was high among children under the age of 7 and were related to ethnic and regional differences in the areas.Specific genes were proliferated along with the extension of time.Our data provided valuable information on prevention and genetic studies on thalassaemia in the minorities of Xishuangbanna and Dehong in Yunnan province.

OBJECTIVETo investigate the epidemiological status of glucose-6-phosphate dehydrogenase (G6PD) deficiency among children from Yunnan with unique ethnic origins.

METHODSDNA samples from 11759 children were tested with fluorescent spot test, G6PD/6PGD quantitative ratio assay and hemoglobin electrophoresis.

RESULTSThe detection rate of G6PD deficiency was 2.5%, for which boys were significantly greater than girls (3.5% vs. 1.4%, P<0.05). Significant differences were also detected among children from different ethnic groups and different regions. For ethnic Han Chinese, the detection rate was 0.7%, which was lower than the majority of ethnic minorities. By regression analysis, altitude of residence and family history both have significant influence on the calculated rate.

CONCLUSIONOccurrence of G6PD deficiency seems to be influenced by gender. It also varies substantially between different ethnic groups as well as regions, e.g., more common in south. It also showed a declining trend after years of diagnosis and intervention. This survey may provide a valuable basis for counseling of G6PD deficiency in Yunnan.

Child ; Child, Preschool ; China ; ethnology ; Female ; Glucosephosphate Dehydrogenase Deficiency ; ethnology ; genetics ; Humans ; Infant ; Infant, Newborn ; Logistic Models ; Male

BACKGROUNDDecoy receptor 3 (DcR3) binds to Fas ligand (FasL) and inhibits FasL-induced apoptosis. The receptor is overexpressed in hepatocellular carcinoma (HCC), and it is associated with the growth and metastatic spread of tumors. DcR3 holds promises as a new target for the treatment of HCC, but little is known regarding the molecular mechanisms underlying the oncogenic properties of DcR3. The present work, therefore, examined the role of DcR3 in regulating the growth and invasive property of liver cancer cell HepG2.

METHODSHepG2 cells were stably transfected with lentivirus-based short hairpin RNA vector targeting DcR3. After the knockdown of DcR3 was confirmed, cell proliferation, clone formation, ability of migrating across transwell membrane, and wound healing were assessed in vitro. Matrix metalloproteinase-9 (MMP 9) and vascular epithelial growth factor (VEGF)-C and D expressions of the DcR3 knockdown were also studied. Comparisons between multiple groups were done using one-way analysis of variance (ANOVA), while pairwise comparisons were performed using Student's t test. P< 0.05 was regarded statistically significant.

RESULTSDcR3 was overexpressed in HepG2 compared to other HCC cell lines and normal hepatocyte Lo-2. Stable knockdown of DcR3 slowed down the growth of HepG2 (P < 0.05) and reduced the number of clones formed by 50% compared to those without DcR3 knockdown (P < 0.05). The knockdown also reduced the migration of HepG2 across transwell matrix membrane by five folds compared to the control (P < 0.05) and suppressed the closure of scratch wound (P < 0.05). In addition, the messenger RNA levels of MMP 9, VEGF-C, and VEGF-D were significantly suppressed by DcR3 knockdown by 90% when compared with the mock control (P < 0.05).

CONCLUSIONSLoss of DcR3 impaired the growth and invasive property of HCC cell line of HepG2. Targeting DcR3 may be a potential therapeutic approach for the treatment of HCC.

Analysis of Variance ; Cell Movement ; genetics ; physiology ; Cell Proliferation ; genetics ; physiology ; Hep G2 Cells ; Humans ; Matrix Metalloproteinase 9 ; genetics ; metabolism ; RNA, Small Interfering ; genetics ; Receptors, Tumor Necrosis Factor, Member 6b ; genetics ; metabolism ; Vascular Endothelial Growth Factor A ; genetics ; metabolism

Animals ; Chelating Agents ; pharmacology ; Lead ; toxicity ; Learning ; drug effects ; Memory Disorders ; drug therapy ; Mice ; Morus ; chemistry ; Plant Extracts ; chemistry ; pharmacology ; Random Allocation ; Succimer ; pharmacology