1.Observation on Therapeutic Effect of SNMC in Treating Dermatosis of Enzema and Dermatitis
Chunyan HUANG ; Nengxing LIN ; Yating TU ; Xin LIAN ; Changzhen HUANG
China Pharmacy 2001;0(07):-
OBJECTIVE:To approach the therapeutic effect of SNMC in treating dermatosis of enzema and dermati-tis.METHODS:190patients with dermatosis of enzema and dermatitis were randomly allocated to therapy group and control group in terms of specific diseases.The control group were only treated with drug for external use,while the therapy group were treated with SNMC as well as drug for external use.RESULTS:The recovery rate and effective rate in the therapy group significantly(P
2.Interaction between CYP450 enzymes and metabolism of traditional Chinese medicine as well as enzyme activity assay.
Tu-lin LU ; Lian-lin SU ; De JI ; Wei GU ; Chun-qin MAO
China Journal of Chinese Materia Medica 2015;40(18):3524-3529
Drugs are exogenous compounds for human bodies, and will be metabolized by many enzymes after administration. CYP450 enzyme, as a major metabolic enzyme, is an important phase I drug metabolizing enzyme. In human bodies, about 75% of drug metabolism is conducted by CYP450 enzymes, and CYP450 enzymes is the key factor for drug interactions between traditional Chinese medicine( TCM) -TCM, TCM-medicine and other drug combination. In order to make clear the interaction between metabolic enzymes and TCM metabolism, we generally chose the enzymatic activity as an evaluation index. That is to say, the enhancement or reduction of CYP450 enzyme activity was used to infer the inducing or inhibitory effect of active ingredients and extracts of traditional Chinese medicine on enzymes. At present, the common method for measuring metabolic enzyme activity is Cocktail probe drugs, and it is the key to select the suitable probe substrates. This is of great significance for study drug's absorption, distribution, metabolism and excretion (ADME) process in organisms. The study focuses on the interaction between TCMs, active ingredients, herbal extracts, cocktail probe substrates as well as CYP450 enzymes, in order to guide future studies.
Animals
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Cytochrome P-450 Enzyme Inhibitors
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metabolism
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pharmacology
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Cytochrome P-450 Enzyme System
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chemistry
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metabolism
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Drugs, Chinese Herbal
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metabolism
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pharmacology
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Enzyme Activation
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drug effects
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Enzyme Activators
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metabolism
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pharmacology
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Humans
3.In vitro recombination and identification of mutated fragment corresponding to regulation region of mtrR gene of Neisseria gonorrhoeae.
Changzheng, HUANG ; Nengxing, LIN ; Yating, TU ; Xin, LIAN ; Jian, KANG ; Li, ZHU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(5):608-10
A site-directed mutant DNA fragment was synthesized and transfected into clinical Neisseria gonorrhoeae (NG) stains to construct the transformants that contained the corresponding mutagenesis of regulation region of mtrR gene. According to the technique of gene splicing by overlap extension (SOEing), a DNA segment with specific mutagenesis was constructed by two-step polymerase chain reaction (PCR). The mutation fragments EF could be used for the next experiment in which the mutation NG strains were induced. By comparing the recombinant EF fragments to the corresponding DNA fragments of clinical NG strains, 2 of these were not compatible completely. The results of sequencing revealed that there was a 9 bp deletion between the 45 to 54 inverted repeat sequence localized within the mtrR promoter. It can be confirmed that the fragments EF are the specifically designed mutant fragments.
Bacterial Proteins/*genetics
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DNA Fragmentation
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DNA, Bacterial/genetics
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Mutagenesis, Site-Directed
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Neisseria gonorrhoeae/*genetics
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Neisseria gonorrhoeae/metabolism
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Recombination, Genetic
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Repressor Proteins/*genetics
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Sequence Deletion
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Transfection
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Transformation, Bacterial
4.Study on effects of sulfur fumigation on chemical constituents of Chrysanthemum morifolium cv. Boju.
You-lian LI ; Shan WANG ; Jing-jing ZHU ; Wei-hao WANG ; Shi-xie XIANG ; Wei-hong FENG ; Liang-mian CHEN ; Zhi-min WANG ; Tu-lin LU
China Journal of Chinese Materia Medica 2015;40(13):2624-2628
A comprehensively comparison of the chemical profiles between sun-drying BJ (NBJ) and sulfur-fumigated BJ (SBJ) was conducted by HPLC analysis and the discrepant peaks were identified or tentatively assigned by HPLC-ESI-MSn. A total of 32 chemical components were used for qualitative comparison. Meanwhile, a quantitative comparison of BJwere conducted by HPLC analysis and determining seven compounds from 3 NBJ and 3 SBJ samples dramatic chemical changes were found. After sulfur fumigation, the contents of flavonoids glycosides and phenolic acids were remarkably reduced, but the contents of flavonoids aglycones were significantly increased. Multivariate statistics, including principle component analysis (PCA) and partial least squares discriminate analysis (PLS-DA) were used to investigate the potential damaging effect of sulfur-fumigating process. The PCA score plots showed six samples were clearly classified into the sun-drying and sulfur-fumigating groups. And according to VIP >1, the most important chemical markers were apigenin, luteolin and 3,5-dicaffeoylquninic acid which could be used to distinguish NBJ and SBJ samples. Combining the results of qualitative and quantitative analysis, it showed that the sulfur fumigation has a significant effect on BJ.
Chromatography, High Pressure Liquid
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Chrysanthemum
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chemistry
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Fumigation
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Least-Squares Analysis
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Principal Component Analysis
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Sulfur
5.Case control study of fractures-dislocations of ankle joint with conservative and operative treatment.
Song-Tu ZHANG ; Yi-Rong LIN ; Lian-Yuan CHEN
China Journal of Orthopaedics and Traumatology 2010;23(10):760-763
OBJECTIVETo compare the clinical efficacy of grade III, IV supination-eversion fractures-dislocations of ankle joint between manipulative treatment and operative treatment.
METHODSFrom September 2007 to December 2008, the clinical data of 60 patients with grade III, IV supination-eversion fractures-dislocations of ankle joint were retrospectively analyzed. There were 32 males and 28 females, ranging in age from 18 to 70 years with an average age of 38.17 years. All patients were respectively treated with manipulative treatment (conservative group, 30 cases) and operative treatment (operative group, 30 cases). The joint function was compared with Mazur standard; the reduction and shifting of fractures were observed with X-ray; the hospitalization day and the therapeutic cost were compared between two groups.
RESULTSAll patients were followed up with an average of 15.27 months (ranged, 6 to 25 months). In conservative group, 16 cases got excellent result in joint function, 10 good, 3 fair, 1 poor; in operative group, 20 cases got excellent result, 8 good, 2 fair, 0 poor. In conservative group in the X-ray showed 25 cases obtained excellent and good reduction, 4 fair, 1 poor; and in operative group in the X-ray showed 28 cases obtained excellent and good reduction, 2 fair, 0 poor. There was no significant difference at the joint function and X-ray film after treatment between two groups (P > 0.05). The hospital day was respectively (7.87 +/- 3.34), (17.37 +/- 4.64) d in conservative group and operative group; and the therapeutic cost was respectively (2 506.67 +/- 649.10), (11 473.33 +/- 1 564.90) yuan. There was significant difference at hospital day and therapeutic cost between two groups (P < 0.05).
CONCLUSIONConservative treatment and operative treatment can both reach a very good result in treating grade III, IV supination-eversion fractures and dislocations of ankle joint. However, conservative treatment has advantage of high safety factor, low therapeutic cost, can reduce medical costs for patients.
Adolescent ; Adult ; Aged ; Ankle Joint ; physiopathology ; Case-Control Studies ; Female ; Fracture Fixation, Internal ; Fractures, Bone ; surgery ; Humans ; Joint Dislocations ; surgery ; Male ; Middle Aged ; Supination ; X-Ray Film ; X-Rays ; Young Adult
6.In vitro Recombination and Identification of Mutated Fragment Corresponding to Regulation Region of mtrR Gene of Neisseria Gonorrhoeae
Changzheng HUANG ; Nengxing LIN ; Yating TU ; Xin LIAN ; Jian KANG ; Li ZHU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(5):608-610
A site-directed mutant DNA fragment was synthesized and transfected into clinical Neisseria Gonorrhoeae (NG) stains to construct the transformants that contained the corresponding mutagenesis of regulation region of mtrR gene. According to the technique of gene splicing by over-lap extension (SOEing), a DNA segment with specific mutagenesis was constructed by two-step po-lymerase chain reaction (PCR). The mutation fragments EF could be used for the next experiment in which the mutation NG strains were induced. By comparing the recombinant EF fragments to the corresponding DNA fragments of clinical NG strains, 2 of these were not compatible completely. The results of sequencing revealed that there was a 9 bp deletion between the 45 to 54 inverted repeat se-quence localized within the mtrR promoter. It can be confirmed that the fragments EF are the specifi-cally designed mutant fragments.
7.Select alternative technology to sulfur fumigation for Astragali Radix based on multi-index method.
Lin LI ; Lin JI ; Xue CHENG ; Lian-Lin SU ; Tu-Lin LU ; Jin-Guo XU ; De JI
China Journal of Chinese Materia Medica 2016;41(14):2581-2586
Sulfur fumigation method is often used in the habitat processing of Astragali Radix in order to make it mothproof, mildewproof and easy to dry; however, this method has great influence on the inherent quality of Astragali Radix. Therefore, it is urgent to develop the alternative technology to sulfur fumigation. In the present paper, appearance characteristics, content of active ingredients, containing water, extracting content and microbial content in Astragali Radix were taken as the indexes to compare the quality of Astragali Radix processed with 9 kinds of processing methods (traditional processing method, sulfur fumigation and 7 kinds of alternative processing technologies). Combined hot air-microwave technology was finally determined as an alternative technology to sulfur fumigation.
8.Effect of Astragali Radix with different sulfur fumigation technologies on immune function in mice.
Xi-Yan DING ; Lin JI ; Xue CHENG ; Lian-Lin SU ; De JI ; Lin LI ; Tu-Lin LU
China Journal of Chinese Materia Medica 2016;41(15):2819-2823
To investigate the effect of Astragali Radix with different sulfur fumigation technologies on immune function in mice, and observe the effect of different Astragali Radix samples on carbon clearance in cyclophosphamide induced immunosuppressed mice, on immune organ weight in immunosuppressed mice and on delayed type hypersensitivity response (DTH) induced by 2,4 dinitrochlorobenzene. Carbon clearance index, phagocytic index, organ index and ear swelling rate were taken as the indexes. The results showed that, all of the Astragali Radix with different sulfur fumigation technologies markedly increased the carbon clearance index K, phagocytic index α, immune organ weight and improved the ability of DTH response in immunosuppressed mice. As compared with the model group, combined hot air-microwave group had the most significant difference, but when other groups were compared with and combined hot air-microwave group, only carbon clearance test had significant difference. From the perspective of pharmacodynamics, the effect of Astragali Radix with different sulfur fumigationon technologies on the immune function of mice was investigated, which provided a reference for the selection of appropriate alternative technology, and also provided guidance for clinical medication.
9.Quality research of Puerariae Lobatae Radix from different habitats with UPLC fingerprint and determination of multi-component content.
Zi-Yan HUANG ; Qian-Neng SHEN ; Ping LI ; Lian-Lin SU ; Li-Hong CHEN ; Tu-Lin LU ; Chun-Qin MAO
China Journal of Chinese Materia Medica 2019;44(10):2051-2058
To establish ultra performance liquid chromatography( UPLC) fingerprint of Puerariae Lobatae Radix from different habitats and simultaneously determine the contents of six isoflavonoids. The UPLC fingerprint analysis and content determination were performed on a Waters ACQUITY UPLC BEH C_(18)( 2. 1 mm×50 mm,1. 7 μm) chromatographic column,with acetonitrile-0. 05% formic acid as mobile phase for gradient elution. The detection wavelength was set at 250 nm; the flow rate was 0. 2 mL·min~(-1); the column temperature was 30 ℃ and the injection volume was 2 μL. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( TCM) was adopted; principal component analysis( PCA) and discriminant analysis by partial least square method( PLS-DA) in Simca-P software were used to identify the differential components in samples from three habitats. The similarity was over 0. 90 in 29 batches of samples,indicating good consistency of the samples. The samples were clustered into 3 categories by PCA and PLS-DA,and six differential components such as puerarin apioside,daidzin,and isoflavoues aglycone were found. The determination results of 6 isoflavones,including 3'-hydroxy puerarin,puerarin,3'-methoxy puerarin,puerarin apioside,daidzin,and isoflavoues aglycone,showed that the content of the same component and the fluctuation range between different components were all different among different habitats. The total content of 6 isoflavones from different regions was Anhui 11. 21% >Henan 10. 97% >Shannxi 9. 38%. The establishment of UPLC fingerprint combined with simultaneous determination of 6 active components provides a more comprehensive reference for quality control and quality evaluation of Puerariae Lobatae Radix.
Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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chemistry
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Ecosystem
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Flavonoids
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analysis
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Phytochemicals
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analysis
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Plant Roots
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chemistry
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Pueraria
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chemistry
10.Historical evolution and research advance in processing adjuvant--vinegar.
Yu LI ; Lian-Lin SU ; De JI ; Chun-Qin MAO ; Xiu-Mei CUI ; Cheng-Lin SUI ; Tu-Lin LU ; Xiao-Li ZHAO
China Journal of Chinese Materia Medica 2021;46(16):4083-4088
Processing of Chinese medicinals with vinegar is one of the characteristic processing techniques. Vinegar is vital for the quality of vinegar-processed decoction pieces. However, there have been no specified standards for adjuvants. Through consulting relevant literature and monographs, we comprehensively reviewed the historical evolution of processing with vinegar in records, selection and application of vinegar, and summarized the relevant standards and current status of vinegar as an adjuvant in China. According to the records in literature, vinegar is effective in activating blood, moving qi, dispersing blood stasis, removing toxin, promoting appetite, and nourishing the liver. Traditionally, rice vinegar is chosen in processing. Nowadays, the vinegar made from rice under solid-state fermentation should be chosen. At present, only food standards can be taken for reference for vinegar in the processing. Integrative and specific inspection indicators are lacking, so the standards for adjuvants need to be improved urgently. In addition, the inadequacy in quality control and management is also a major problem to be solved. Through literature research, we reviewed the historical evolution and research advance in vinegar to provide a reference for the standardization and further research of vinegar used in the Chinese medicinal processing.
Acetic Acid
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Adjuvants, Pharmaceutic
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Drugs, Chinese Herbal
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Oryza
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Quality Control