OBJECTIVE: To establish an HPLC method for the determination of Baicalin in Tanreqing injection.METHODS: The determination was performed by HPLC using an CLC-ODS C18 column(150 mm?6.0 mm,5 ?m),with mobile phase consisted of methanol-water(47∶53) at a flow rate of 0.5 mL?min-1 and a detection wavelength of 280 nm.RESULTS: The calibration curve for Baicalin was linear in the concentration range of 2.53～12.65 ?g?mL-1(r=0.999 8).The average recovery was 99.61%(RSD=0.35%,n=9).CONCLUSION: The method is simple,rapid and applicable for the quality control of Tanreqing injection.

Objective To evaluate correlations between CT features and clinical activity index of Crohn’s disease(CD).Methods The CTE datasets from 37 patients with moderate active,severe active and remission stages CD were retrospectively analyzed.To ob-serve imaging features of intestinal wall,parenteral and complications.And analyze whether there was an association between imaging findings and clinical activity.Results A total of 147 bowel segments in 37 cases were involved.Wall thickening (intestinal wall≥4 mm)were observed in 121 segments.An average thickness was (12.1±3.2)mm.There are 42 bowel segments of trilaminar mural stratifica-tion,71 of the double-layed stratification and 34 of non-stratification.Enlarged mesenteric lymph nodes were observed in 20 patients. Comb sign was observed in 27 patients.Higher density of surrounding fat appeared in 22 cases.The other 37 cases inluced 4 cases with abdominal abscess,7 cases with fistula,1 6 cases with intestinal obstruction or stenosis and 1 7 cases of perianal disease.The in-testinal stratification,wall thickening,comb sign,mesenteric fat density increased,abdominal abscess,intestinal stenosis and obstruc-tion were closely associated with clinical activity.Conclusion CTE images could provide information about intestinal wall,parenteral changes and complications of Crohn’s disease,and effectively help identifying remission and active stages of Crohn’s disease.

BACKGROUND: L-arginine (L-Arg) is the precursor of endogenous nitric oxide (NO), which joins the pain regulation of peripheral myeloid level and above.OBJECTIVE: To study the role and mechanism of NO in nucleus raphe magnus (NRM) in pain regulation and electroacupuncture analgesia.DESIGN: A random control experiment on rats.SETTING: Physiology Department of Medical College of Xianning College.MATERIALS: The experiment was done during May 2002 to March 2003in the Physiology Department of Medical College of Xianning College. The 63 Wistar rats were randomized into 7 groups with 9 in each. ①. Five groups for the experiment of L-Arg dose-effect relationship: Normal saline group and L-Arg 1, 2, 4, 8 mmol groups. ②. Two groups for the experiment of relationship between L-Arg and electroacupuncture analgesia: Normal saline + electroacupuncture group and L-Arg + electroacupuncture group.METHODS: ①Experiment of L-Arg dose-effect relationship: Normal saline group was injected normal saline. L-Arg 1, 2, 4, 8 mmol groups were injected L-Arg 1, 2, 4, 8 mmol respectively. The volume was all 1.5 μL.Then, the hot water (50±0.5) ℃ was used to stimulate the tail once every 10 minutes to cause tail flick for testing the pain threshold. The observation was carried out continuously for 90 minutes. ②. Experiment of relationship between L-Arg and electroacupuncture analgesia: The two groups were injected normal saline and L-Arg 8 mmol respectively. The volume was all 1.5 μL. Ten minutes later, Zusanli (ST 36) of both posterior legs of rats were punctured with electric stimulation. The frequency was 4-16 Hz, and the intensity in an increasing order of 1, 2, 3V given 10 minutes of each. The electroacupuncture was totally 30 minutes, during which, testing the pain threshold 3 times. After needling, the pain threshold was still tested once every 10 minutes, till 90 minutes after injecting L-Arg.MAIN OUTCOME MEASURES: Pain threshold at different time points.RESULTS: The 63 rats entered the result analysis. ① The pain threshold of L-Arg 1 mmol group at all time points was lower than that of the normal saline group, but without statistical significance (P ＞ 0.05). The pain threshold of L-Arg 2, 4, 8 mmol groups was much lower than that of the normal saline group at all time points (P ＜ 0.001), and the effect was lasting to 80 minutes after injecting L-Arg, and with the increase of L-Arg density, the amplitude of pain threshold lowering was increased. ② The pain threshold of L-Arg + electroacupuncture group was much lower than that of the normal saline + electroacupuncture group at the time points of 20-50 minutes (P ＜ 0.01).CONCLUSION: ① The L-Arg injection to NRM can lower the pain threshold and there is a dose-dependent effect in it. ② The electroacupuncture can raise the pain threshold, and the L-Arg injection can weaken the effect of electroacupuncture analgesia. ③ NO in NRM joins in the pathological and physiological processes of pain and electroacupuncture analgesia. Its increase can lower the pain threshold greatly.

To explore the clinical application and value of small incisions in the treatment of benign thyroid diseases with endoscopic assistance.A total of 57 cases with thyroid nodules were operated under the assistance of ultrasonic scalpel and endoscope with the lifting-up of special instrument and small neck incisions.However one malignant case was converted iuto open surgery according to frozen sections.The other 56 cases were successfully operated.The mean operative duration was 42 min and postoperative stay 2 -3 days.All incisions achieved primary healing and the length range was 1.5 - 3.5 cm.Except for transient hoarseness in 1 case,there was no such complications as choking,lip paralysis or hemorrhage,etc.All were discharged uneventfully.This surgical approach shall be recommended for its merits of miniinvasiveness,less hemorrhage,fewer complications and wider applications.

OBJECTIVE:To provide reference for rational use of opioids analgesics. METHODS:1 882 prescriptions of opi-oids analgesics for cancer pain collected from our hospital in 2014 were analyzed by defined daily dose(DDD)and drug utilization index(DUI). RESULTS:The diseases of opioids analgesics prescriptions for cancer pain in our hospital in 2014 were mainly lung cancer,accounting for 42.19%. Commonly used opioids analgesics included Morphine sulfate sustained-release tablets,Oxycodone Hydrochloride sustained-release tablets and so on,and their DUI were all below 1.0. Top one drug in the list of amount and con-sumption sum was Morphine sulfate sustained-release tablets,and its main dosage form were tablets,injection and patches,in which tablets occupied the largest proportion,reaching 97.30%. CONCLUSIONS:The application of opioids analgesics for cancer pain in our hospital is basically rational in terms of drug types,dosage form and route of administration,but the dose of opioids an-algesics is small and their DUI is lower than 1;at the same time,there are a few irrational prescription.

Praziquantel ( PQT ) concentrations in plasma after iv 20 mg/kg decayed rapidly with tip of 0.36 h. The absorption of PQT was rapid following the intramuscular doses of 10,20,40mg/kg or intragastic dose of 100mg/kg, but the phase of elimination was much longer than that after iv. Both of MAT1m and MATig were greater than MRTiv. The bioavailability of ig was 13.2%, suggesting a strong first-pass effect. The kinetics of PQT elimination was linear after intramuscular dose of either 10 or 20 mg/kg, but nonlinear process was found when the dose was increased to 40mg/kg.By any route of iv, im and ig administration, the concentrations of PQT in the bile were much lower than the peripheral plasma concentrations and changed in parallel to the later with high levels after iv, medium levels after im and much low levels after ig.

OBJECTIVE:To establish HPLC method for the determination the centent of tanshinone Ⅱ_A in danshen shuxin capsule.METHODS:The determination was performed on Shim-pack CLC-ODS column,the mobile phase consisted of methanol-water(15∶5),the detection wavelength of270nm,column temperature at40℃,a flow rate of1.5ml/min and.RE_ SULTS:The calibration curve of tanshinone Ⅱ_A is linear when its concentration range was with in 0.05125～0.25625?g/ml(r=0.9996,n=5),the average recovery was 99.05%(RSD=0.92%).CONCLUSION:This method is convenient in operation,precise,reliable in results,and it can be used as quality control for danshen shuxin capsule.

In this paper, the common errors in medical devices test reports are classified and analyzed. And then the main 11 influence factors for these inspection report errors are summarized. The hierarchy model was also developed and verified by presentation data using MATLAB. The feasibility of comprehensive weights quantitative comparison has been analyzed by using the analytic hierarchy process. In the end, this paper porspects the further research direction.
Equipment Failure Analysis ; Equipment and Supplies ; standards ; Models, Theoretical

Objective To explore ultrasonographic characteristics and prognosis of cesarean scar pregnancy in the ultrasound.Methods To conducted a retrospective study of 8 cases of diagnoses of cesarean scar pregnancy in ultrasound in our hospital from January 2013 to August 2015.All cases had ultrasonographic examinations regularly during the pregnancy and were confirmed placenta increta by cesarean section or prenatal MRI (6 cases).The imaging characteristics of 8 cases of pregnant women in early pregnancy by transvaginal ultrasound,and the positions of gestational sac and chorion frondosum were observed.Ultrasonic follow-up results,numbers of cesarean delivery,final diagnosis,and pregnancy outcome were analyzed.Results Eight cases of patients were diagnosed cesarean scar pregnancy,and all of ceses were diagnosed placenta increta by ultrasound from 11 weeks to 24 weeks of gestation.It was found that the positions of chorion frondosum were located in the lower edge of gestational sac in Two-dimensional ultrasound and the chorion frondosum was covered in cesarean section scar.The echoes of proliferous chorion frondosum were stronger than the rest of chorion leve and decidua reflexa,and the thickness of proliferous chorion frondosum were thicker than the rest of chorion leve and decidua reflexa.In the follow-up ultrasound of 8 cases in the second trimester,the original position of chorion frondosum which covered and the position of placenta were roughly similar.The position of placenta did not move up with the gestational weeks,in addition,appeared as placenta previa and covered in cesarean section scar.Five patients had strong childbearing willing of continue to conceive,one case of which needed hysterectomy,four of which underwent cesarean section delivery with alive births after using bilateral iliac arteries balloon occlusion.The rest 3 cases underwent induction of labor after using bilateral iliac arteries balloon cclusion.Conclusions Cesarean scar pregnancy in first pregnancy may be develop as placenta accreta in second and third trimester pregnancy.If chorion frondosum was observed to cover cesarean section scar by ultrasound in early pregnancy,scar pregnancy diagnosis was established.Pregnant women who have strong willing to continue their pregnancy should be pay more attentin to thiers placenta previa and placenta percreta in second and third trimester pregnancy.Close follow-up are needed.

OBJECTIVE:To approach the therapeutic effect of SNMC in treating dermatosis of enzema and dermati-tis.METHODS:190patients with dermatosis of enzema and dermatitis were randomly allocated to therapy group and control group in terms of specific diseases.The control group were only treated with drug for external use,while the therapy group were treated with SNMC as well as drug for external use.RESULTS:The recovery rate and effective rate in the therapy group significantly(P