1.A highly sensitive chemiluminescence assay for superoxide detection and chronic granulomatous disease diagnosis
Tsuyoshi Yamazaki ; Chikage Kawai ; Akira Yamauchi ; Futoshi Kuribayashi
Tropical Medicine and Health 2011;39(2):41-45
Reactive oxygen species (ROS) produced by neutrophils are crucial for defense against infectious diseases, and the adequate measurement of ROS levels is an important way to evaluate the possibility of infections. The fluorescent probe dihydrorhodamine 123 has been applied exclusively to the measurement of ROS thus far. We developed a novel method for detecting ROS, which utilizes the chemiluminescent probes Luminol and Diogenes. The new method quantitatively detects ROS produced by as few as 10 to 104 neutrophils. Furthermore, this method can detect ROS levels in one microliter of whole blood or ROS produced by Epstein-Barr immortalized B lymphocytes. This method will be valuable for prompt diagnosis of neonatal chronic granulomatous diseases in which neutrophils aberrantly produce superoxide.
2.Utility of topical epinephrine for determining the resection range of eyelid sebaceous carcinoma with dermatitis.
Tomoki KIUCHI ; Yusuke SHIMIZU ; Shun YAMAZAKI ; Tsuyoshi AWAZAWA ; Kazuo KISHI
Archives of Plastic Surgery 2018;45(3):289-290
No abstract available.
Dermatitis*
;
Epinephrine*
;
Eyelids*
3.Accuracy of Pedicle Screw Placement in Scoliosis Surgery: A Comparison between Conventional Computed Tomography-Based and O-Arm-Based Navigation Techniques.
Toshiaki KOTANI ; Tsutomu AKAZAWA ; Tsuyoshi SAKUMA ; Kayo KOYAMA ; Tetsuharu NEMOTO ; Kento NAWATA ; Atsuro YAMAZAKI ; Shohei MINAMI
Asian Spine Journal 2014;8(3):331-338
STUDY DESIGN: Retrospective study. PURPOSE: We compared the accuracy of O-arm-based navigation with computed tomography (CT)-based navigation in scoliotic surgery. OVERVIEW OF LITERATURE: No previous reports comparing the results of O-arm-based navigation with conventional CT-based navigation in scoliotic surgery have been published. METHODS: A total of 222 pedicle screws were implanted in 29 patients using CT-based navigation (group C) and 416 screws were implanted in 32 patients using O-arm-based navigation (group O). Postoperative CT was performed to assess the screw accuracy, using the established Neo classification (grade 0: no perforation, grade 1: perforation <2 mm, grade 2: perforation > or =2 and <4, and grade 3: perforation > or =4 mm). RESULTS: In group C, 188 (84.7%) of the 222 pedicle screw placements were categorized as grade 0, 23 (10.4%) were grade 1, 11 (5.0%) were grade 2, and 0 were grade 3. In group O, 351 (84.4%) of the 416 pedicle screw placements were categorized as grade 0, 52 (12.5%) were grade 1, 13 (3.1%) were grade 2, and 0 were grade 3. Statistical analysis showed no significant difference in the prevalence of grade 2.3 perforations between groups C and O. The time to position one screw, including registration, was 10.9+/-3.2 minutes in group C, but was significantly decreased to 5.4+/-1.1 minutes in group O. CONCLUSIONS: O-arm-based navigation facilitates pedicle screw insertion as accurately as conventional CT-based navigation. The use of O-arm-based navigation successfully reduced the time, demonstrating advantages in the safety and accuracy of pedicle screw placement for scoliotic surgery.
Classification
;
Humans
;
Prevalence
;
Retrospective Studies
;
Scoliosis*
;
Spine
;
Surgery, Computer-Assisted