Background: There is an attitude to prefer bio preparation made from raw materials of plants, animals and minerals to medicines made under synthetic method in today's world. Also, traditional Mongolian medicine is rising and studying in detail medicinal plants which are used in traditional Mongolian medicine from ancient time. As well as it interested in defining dependence between substances and its biological activity.
Therefore, we have chosen Dracocephalum foetidum Bge which has been used for the treatment of various inflammatory conditions such as oral cavity diseases, rheumatic edema and wounds.
In addition the leaves and flowers of Dracocephalum foetidum Bge are used as traditional medicine among Mongolian nomads to wash their faces and hands to prevent bacterial and fungal infections. The flowers of the plant are used for fever and suppurative diseases.
There have been few studies on D. foetidum, and the only report on chemical constituents, mainly focused on the essential oil components that are effective against bacteria. Therefore, it is important to study the specific chemical characteristics and physiological roles of constituents of D.foetidum.
The sediments of the genus are found in the northern hemisphere of Lamiaceae, which grows over 60 species. In Mongolia, there are 17 species, which are abundant in the northern and eastern parts of the country. Purpose and objectives: We aimed to study the Dracocephalum foetidum Bge plant, which was used in traditional Mongolian medicine, and has set the following objectives.
These include:
• Isolation and purification of chemical constituents from aerial parts of Dracocephalum foetidum Bge
• Structure elucidation of isolated compounds Innovative research: We have isolated and structure elucidated total of 6 compounds including Rosmarinic acid, caffeic acid trimer, acacetin, acacetin-7-O-β-D-glucuronide, acacetin -7-0- (3-O-malonyl) -β-D-glucuronide.
From this plant, the only glycosides that have not been distinguished before are the acacetins and lutleolins. Materials and methods: o ¹H NMR (400 MHz) and ¹³C NMR (100 MHz), ¹H-¹H COSY, HMQC (optimized for ¹J_C-H =145 Hz), and HMBC (optimized for ⁿJ_C-H = 8 Hz) spectra were recorded on a JNM-AL400 FT-NMR spectrometer (Jeol Ltd., Tokyo, Japan), and chemical shifts are given as δ values with TMS as an internal standard. HRFABMS data were obtained on a JMS700 mass spectrometer (Jeol Ltd.), using either an m-nitrobenzyl alcohol or glycerol matrix.
○ A porous polymer gel (Diaion HP-20, 60 x 300 mm, Mitsubishi Chemical Co., Tokyo, Japan) and octadecyl silica (ODS) (Cosmosil 140 C₁₈-OPN, 150 g, Nacalai Tesque, Kyoto, Japan) were used for column chromatography.
○ Preparative HPLC was performed on a Jasco 2089 and detected with UV at 210 nm (columns, Cosmosil AR-II, 20 x 250 mm, Nacalai Tesque; Cosmosil 5PE-MS, 20 x 250 mm, Nacalai Tesque; Develosil C₃₀-UG-5, 20 x 250 mm, Nomura Chemical Co. Ltd., Aichi, Japan; Mightysil RP-18 GP, 10 x 250 mm, Kanto Chemical Co. Inc., Tokyo, Japan). Conclusions
1. A total of six compounds isolated and purified from acetone extract of aerial parts of Dracocephalum foetidum Bge. using column chromatography and HPLC method.
2. Structure elucidation of the molecular structure of the compounds using the nuclear magnetic resonance and mass spectroscopic methods.
Rosmarinic acid (1), caffeic acid trimer (2), acacetin (3), acacetin-7-O-β-D-glucuronide (4), acacetin -7-0- (3-O-malonyl)-β-D-glucuronide (5), luteolin (6)