1.Peripheral blood differential count of white blood cells in blood donor
Tsendsuren S ; Gansukh Ch ; Khongorzul T ; Enkhsaikhan L ; Erdenebayar N ; Nyambayar D ; Tsogtsaikhan S ;
Mongolian Medical Sciences 2020;193(3):3-10
Background:
Establishment of quantitative reference intervals of white blood cells and its subpopulations using
a high accuracy analytic system is essential for clinical medicine, public health, and anthropology.
We are unable to identify peer-reviewed literature sources describing white blood cell counts and
their subpopulations using monoclonal antibodies to specific surface antigens in healthy Mongolians.
This study aimed to measure the counts of white blood cells and their subpopulations in healthy
Mongolians using flowcytometry.
Materials and Methods:
The absolute number (cell/L) of leukocytes (CD45+), granulocytes, monocytes and lymphocytes were
measured by Magnetic Activated Cell Sorting Assay (MACSQuant Analyzer 10) in 287 blood donors
(158 males and 129 females) 17-64 years of age (mean age 33.1±12.4). Peripheral blood samples
were collected at the time of blood donation at the National Center for Transfusion Medicine.
Results
The mean values of leukocytes and granulocytes were lower in donors over 30 years of age (ANOVA:
F=4.408, p=0.002 and F=5.685, p=0.001) and regression analysis demonstrated indirect correlation
between counts of these cells and age of donors (r= - 0.198, p=0.001 and r=-0.221, p=0.001,
respectively). Gender-related differences in white blood cell counts were not found.
Mean value of lymphocyte count in donors investigated in spring (May and March, n = 87; 2224.6±775.3) was significantly higher than those in winter (December – February, n=180; 1613.2±454.3, p=0.001) and autumn (October, n=20; 1576.1±438.6, p= 0.001).
Comparing of our findings with the data from available literature shown that healthy Mongolians
have lower leukocyte count compared with Koreans, Chinese Han population and lower mean value
of lymphocyte count comparing with Korean, Chinese Han population, and Arabian (Saudi Arabia)
populations.