Parasitic diseases are widely distributed throughout the world. Recently, travel abroad and migration from abroad are increasing in Korea. Therefore, it is necessary to appropriately control imported parasitic disease. The drugs for the treatment of the parasitic diseases that can be imported from abroad are reserved by the government. To guide proper treatment of parasitic diseases, recommended chemotherapy focused on these reserved drugs has been introduced. The diseases reviewed in this article include malaria, babesiosis, toxoplasmosis, leishmaniasis, Chagas disease, African sleeping sickness, filariasis, angiostrongyliasis, and fascioliasis. Because most of the parasitic diseases produce severe illness or fatal results, rapid and accurate diagnosis is important and following fully the recommended therapy is needed. The recommended drug therapy changes from time to time due to various factors, so always recognizing and applying the latest therapy and is very important.
Animals ; Babesiosis ; Chagas Disease ; Fascioliasis ; Filariasis ; Korea ; Leishmaniasis ; Malaria ; Parasitic Diseases ; Strongylida Infections ; Toxoplasmosis ; Trypanosomiasis, African

Trypanosoma brucei, a protozoan parasite, causes sleeping sickness in humans and Nagana disease in domestic animals in central Africa. The trypanosome surface is extensively covered by glycosylphosphatidylinositol (GPI)-anchored proteins known as variant surface glycoproteins and procyclins. GPI anchoring is suggested to be important for trypanosome survival and establishment of infection. Trypanosomes are not only pathogenically important, but also constitute a useful model for elucidating the GPI biosynthesis pathway. This review focuses on the trypanosome GPI biosynthesis pathway. Studies on GPI that will be described indicate the potential for the design of drugs that specifically inhibit trypanosome GPI biosynthesis.
Animals ; Biosynthetic Pathways ; Glycosylphosphatidylinositols/*biosynthesis/chemistry ; Humans ; Protozoan Proteins/genetics/metabolism ; Trypanosoma brucei brucei/chemistry/genetics/*metabolism ; Trypanosomiasis, African/*parasitology

Trypanosoma brucei, a protozoan parasite, causes sleeping sickness in humans and Nagana disease in domestic animals in central Africa. The trypanosome surface is extensively covered by glycosylphosphatidylinositol (GPI)-anchored proteins known as variant surface glycoproteins and procyclins. GPI anchoring is suggested to be important for trypanosome survival and establishment of infection. Trypanosomes are not only pathogenically important, but also constitute a useful model for elucidating the GPI biosynthesis pathway. This review focuses on the trypanosome GPI biosynthesis pathway. Studies on GPI that will be described indicate the potential for the design of drugs that specifically inhibit trypanosome GPI biosynthesis.
Animals ; Biosynthetic Pathways ; Glycosylphosphatidylinositols/*biosynthesis/chemistry ; Humans ; Protozoan Proteins/genetics/metabolism ; Trypanosoma brucei brucei/chemistry/genetics/*metabolism ; Trypanosomiasis, African/*parasitology

Trypomastogotes of Trypanosoma brucei were detected from 4 asymptomatic kudus (Tragelaphus strepsiceros) on a game ranch located approximately 45 km north east of Lusaka, Zambia. Blood smears examined from 14 wildlife species comprising of the impala (Aepyceros melampus), Kafue lechwe (kobus leche kafuensis), sable antelope (Hippotragus niger), tsessebe (Damaliscus lunatus), warthog (Phacochoerus aethiopicus), puku (Kobus vardoni), zebra (Equus burchelli), waterbuck (Kobus ellipsiprymnus), bushbuck (Tragelaphus scriptus), reedbuck (Redunca arundinum), wilderbeest (Connochaetes taurinus), hartebeest (Alcephelus lichtensteini), African buffalo (Syncerus caffer), and kudu (Tragelaphus strepsiceros) showed that only the kudu had T. brucei. Although game ranching has emerged to be a successful ex-situ conservation strategy aimed at saving the declining wildlife population in the National Parks, our findings suggest that it has the potential of aiding the re-distribution of animal diseases. Hence, there is a need for augmenting wildlife conservation with disease control strategies aimed at reducing the risk of disease transmission between wildlife and domestic animals.
Animals ; Animals, Wild ; Ruminants/*parasitology ; Trypanosoma brucei brucei/*isolation & purification ; Trypanosomiasis/*diagnosis ; Zambia

Since the time of Jonathan Hutchinson(1858~63), it has been known that over 90% of cases of diffuse interstitial keratitis occur in syphilitic patients. Interstitial keratitis is due to acquired syphilis in 4% of cases and the other 6% of cases due to tuberculosis, sarcoid, trypanosomiasis, onchocerciasis and other rare infections. After the introduction of penicillin by Mahoney et al. in 1943 for syphilis therapy, syphilis epidemy was sharply decreased throughout the world. However, during the middle 1950's the incidence of syphilis began to increase throughout the world. In Korea. the syphilis incidence has been increased since around 1963-1965 in parallel with the world trends(Kim and Lew 1968). A 6 year old Korean girl has been found to have bilateral acute interstitial keratitis and a positive serological test for syphilis.
Child ; Female ; Humans ; Incidence ; Keratitis* ; Korea ; Onchocerciasis ; Penicillins ; Serologic Tests ; Syphilis ; Syphilis, Congenital* ; Trypanosomiasis ; Tuberculosis

Toll-like receptors (TLRs) have emerged as major receptor components of pattern-recognition receptors (PRRs), which are responsible for the recognition of pathogen-associated molecular patterns (PAMPs)-derived pathogenic parasites. This recognition triggers the secretion of a large amount of type I interferons (IFNs), inflammatory cytokines, and chemokines and maturation of immune cells, for effective host defense by eradicating infectious parasites. Both the myeloid differentiation factor 88 (MyD88) and the TIR domain containing the adaptor molecule (TRIF) are involved in these signaling pathways. Here, we review the latest findings on the recognition of the pathogenic parasites and activation of corresponding signaling pathways through TLRs, with special emphasis on the recognition of pathogenic protozoan and helminthes. By highlighting recent progress in these areas, we hope to provide references in future studies not only for the complexity of host-parasite interactions but also for the prevention of the pathogenic parasite infections.
Animals ; Host-Parasite Interactions ; immunology ; Humans ; Immunity, Innate ; immunology ; Malaria, Falciparum ; parasitology ; Plasmodium falciparum ; immunology ; Signal Transduction ; Toll-Like Receptors ; immunology ; Trypanosoma ; immunology ; Trypanosomiasis ; parasitology

Trypanosomiasis is caused by a pathogenic protozoan of the genus Trypanosoma, being Trypanosoma vivax the most important agent for cattle. The aim of the present study was to demonstrate the expansion of T. vivax infection in different mesoregions of Minas Gerais, Brazil, and describe the clinicopathological findings of trypanosomiasis in cattle. The diagnosis was based on visualization of the parasite in blood smears and DNA detection of T. vivax in the blood of live cows and tissues of necropsied animals by the polymerase chain reaction (PCR). Thirty suspected herds were tested, of which 11 were positive for T. vivax. The most frequent clinical signs were anemia, apathy, drop in milk production, weight loss, reproductive disorders, and nervous signs. Concomitant diseases, such as malignant edema, pneumonia and increased cases of mastitis were associated with T. vivax infection. Three cows were necropsied and the most significant findings were low body condition score, pale mucous and spleen with white pulp hyperplasia. The results demonstrated the expansion of T. vivax infection in Minas Gerais, that PCR-associated blood smears are promising for diagnosis, and that other diseases often occur concomitantly to T. vivax infection in regions with trypanosomiasis in cattle.
Anemia ; Animals ; Apathy ; Brazil ; Cattle ; Diagnosis ; DNA ; Edema ; Female ; Hyperplasia ; Mastitis ; Milk ; Parasites ; Parasitic Diseases ; Pneumonia ; Polymerase Chain Reaction ; Ruminants ; Spleen ; Trypanosoma vivax ; Trypanosoma ; Trypanosomiasis ; Weight Loss

The aim of this study was to test the susceptibility of mice to Trypanosoma evansi treated with human plasma containing different concentrations of apolipoprotein L-1 (APOL1). For this experiment, a strain of T. evansi and human plasma (plasmas 1, 2, and 3) from 3 adult males clinically healthy were used. In vivo test used 50 mice divided in 5 groups (A to E) with 10 animals in each group. Animals of groups B to E were infected, and then treated with 0.2 ml of human plasma in the following outline: negative control (A), positive control (B), treatment with plasma 1 (C), treatment with plasma 2 (D), and treatment with plasma 3 (E). Mice treated with human plasma showed an increase in longevity of 40.9+/-0.3 (C), 20+/-9.0 (D) and 35.6+/-9.3 (E) days compared to the control group (B) which was 4.3+/-0.5 days. The number of surviving mice and free of the parasite (blood smear and PCR negative) at the end of the experiment was 90%, 0%, and 60% for groups C, D, and E, respectively. The quantification of APOL1 was performed due to the large difference in the treatments that differed in the source plasma. In plasmas 1, 2, and 3 was detected the concentration of 194, 99, and 115 mg/dl of APOL1, respectively. However, we believe that this difference in the treatment efficiency is related to the level of APOL1 in plasmas.
Adult ; Animals ; Apolipoproteins/blood/*therapeutic use ; DNA, Protozoan/genetics ; Female ; Humans ; Lipoproteins, HDL/blood/*therapeutic use ; Male ; Mice ; Polymerase Chain Reaction ; Trypanocidal Agents/blood/*therapeutic use ; Trypanosoma/drug effects/genetics/*pathogenicity ; Trypanosomiasis/drug therapy/mortality/*parasitology ; Young Adult

Trypanosoma cruzi is the etiological agent of Chagas disease. Epimastigote forms of T. cruzi can be readily cultured in axenic conditions. Ethanol and dimethyl sulfoxide (DMSO) are commonly used solvents employed as vehicles for hydrophobic compounds. In order to produce a reference plot of solvent dependent growth inhibition for T. cruzi research, the growth of epimastigotes was analyzed in the presence of different concentrations of ethanol (0.1–4.0%) and DMSO (0.5–7.5%). The ability of the parasites to resume growth after removal of these solvents was also examined. As expected, both ethanol and DMSO produced a dose-dependent inhibition of cellular growth. Parasites could recover normal growth after 9 days in up to 2% ethanol or 5% DMSO. Since DMSO was better tolerated than ethanol, it is thus recommended to prefer DMSO over ethanol in the case of a similar solubility of a given compound.
Chagas Disease ; Dimethyl Sulfoxide* ; Drug Evaluation, Preclinical ; Ethanol* ; Parasites ; Solubility ; Solvents* ; Trypanosoma cruzi* ; Trypanosoma*

Triatoma rubrofasciata is a wide-spread vector of Chagas disease in Americas. In this study, we completed the mitochondrial genome sequencing of T. rubrofasciata. The total length of T. rubrofasciata mitochondrial genome was 17,150 bp with the base composition of 40.4% A, 11.6% G, 29.4% T and 18.6% C. It included 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and one control region. We constructed a phylogenetic tree on the 13 protein-coding genes of T. rubrofasciata and other 13 closely related species to show their phylogenic relationship. The determination of T. rubrofasciata mitogenome would play an important role in understanding the genetic diversity and evolution of triatomine bugs.
Americas ; Base Composition ; Chagas Disease ; Genes, rRNA ; Genetic Variation ; Genome, Mitochondrial ; Phylogeny ; RNA, Transfer ; Trees ; Triatoma