Diagnosis of contagious caprine pleuropneumonia (CCPP) in Saudi Arabia mainlydepends on clinical signs and post-mortem findings, in addition to limited usage of latexagglutination test (LAT). In this study, a PCR method specific for detection of Mycoplasmacapricolum subspecies capripneumoniae (Mccp) was used as a direct confirmatory methodand to compare it with clinical signs, necropsy lesions and LAT. During the 2016-2017 year,samples of serum, pleural fluid, lung tissue and nasal swab were collected from 40 goats withclinical signs of CCPP, which were selected from goats brought to the veterinary clinic ofQassim University from 18 goat herds and nine localities. Epidemiological data revealed34.1%, 27.8% and 81.6% morbidity, mortality and case fatality rates, respectively. At necropsy,31 of 40 goats (77.5%) were found with lesions matching those of CCPP. Molecular findingssupported the suitability and applicability of PCR as a reliable method to diagnose andconfirm CCPP directly from clinical samples. The disease was confirmed by PCR in 35 goatsout of 40 (87.5%), 15 herds out of 18 (83.3%) and in all localities. Sera of 32 goats (80%) werefound positive by LAT. Four of the five goats and two of the three herds negative by PCR werealso negative by LAT and necropsy examination. Therefore, PCR sensitivity was considered97.2% (35/36). Compared to the claimed high specificity and sensitivity of the used PCRmethod, diagnosis of CCPP based on clinical signs was found less specific and necropsyexamination and LAT were less sensitive. It was concluded that molecular detection of Mccpdirectly in clinical samples should routinely be used to confirm diagnosis of CCPP in theregion of study, prevent economic impact of wrong diagnosis and to hasten control process.

The study evaluated antiviral efficacy of antibiotics- Doxycycline and Kanamycinagainst Japanese encephalitis virus (JEV) infection in vivo. Adult Swiss albino mice (4- 6weeks) were used. Mice were distributed in four groups- control group (A), only drug group(B), JEV infected group (C) and JEV + drug treated group (D). Mice were given intravenousinoculation of JEV strain P20778. Doxycycline was given via intra peritoneal (i. p.) route at 50mg/kg dose. Kanamycin was given to mice via subcutaneous (s. c.) route at 20 mg/kg dose. Alldrug dosages were administered at 24 hr, 48 hr and 96 hr post infection (p.i.) twice a day (BID)for upto 14 days. The mice were monitored for 21 days. The viral load was determined byplaque assay. Viral RNA load and cytokine levels were determined. The infected mice died by8 days of infection. Doxycycline treatment at 50 mg/kg dose after 24 hr p.i. lowered diseaseprogression, prolonging the survival of the animals by a week. Antiviral effect was evidentwith reduction of progeny plaque formation. The plaque formation was reduced at 24 hr p.i.compared to virus group. Doxycycline inhibited viral RNA replication. Doxycycline was ableto moderately modulate proinflammatory cytokines. Kanamycin administration was lesseffective. Thus, the studies demonstrated encouraging results in treatment of JEV infectionby Doxycycline. It indicated that Doxycycline delayed the disease progression. Thus, thefindings suggest that Doxycycline could be used as an adjuvant treatment against JE.

Cats are commonly infected by many species of parasites that includes helminths,protozoan parasites, mites and fleas. Parasites affect the health of cats and cause morbidityand mortality, especially in young and old animals. Some species such as Ancylostoma spp.,Opisthorchis felineus and Echinococcus spp. are well-known zoonotic parasites worldwide,that high public health risks. Currently of available data on prevalence of feline parasites inRussia, and published studies provide incomplete information regarding the period ofparasitological study, number of cats examined, origin, gender and life condition of examinedanimals. There are no official veterinary guidelines on parasitological examination available.Moreover low quality of veterinary monitoring and inadequate preventive measures has leadto the high rates of environmental contamination by infested cats’ feces containing helminthseggs and protozoan cysts. This paper reviews the knowledge on feline parasites fauna andthe prevalence in Russia. Practical aspects related to diagnosis, treatment, and controls ofparasitic diseases of cats in Russia are discussed.

The aim of present study was to determine the seroprevalence of Dirofilariaimmitis in dogs and cats from Riyadh, Saudi Arabia. Blood samples were collected by cephalicand jugular venipuncture from 294 dogs and 190 cats from Riyadh. Serum samples weretested against circulating D. immitis antigen using DiroCHEK?, SYNBIOTICS Corporation,San Diego, CA 92127, USA and anti-D. immitis antibodies using Green Spring D. immitis IgGantibody ELISA test kit, Shenzhen Lvshiyuan Biotechnology Co., Ltd. Guangdong, China. D.immitis antigens were detected in 7.82% of the dogs and in 1.58% of the cats, while antibodiesagainst D. immitis were detected in 13.61% of the dogs and 2.11% of the cats. In outdoordogs, antigens of D. immitis were detected in 10.33% while antibodies were detected in16.85%. A significant difference in the seroprevalence of D. immitis antibodies in dogs wasreported in the summer compared to the winter (p<0.05). There was no significant differenceobserved in the seroprevalence between males and females dogs. Older dogs (3-6 years)showed higher prevalence of D. immitis antigen compared to younger dogs, in the contraryD. immitis antibody prevalence was higher in younger dogs (1-3 years) compared to olddogs. In cats, the prevalence of D. immitis antigen and antibody was high in males comparedto the females, however, the difference was not statistically significant. There was no effectfor the season and age in the prevalence of D. immitis in cats.

Canine babesiosis is an important tick-borne protozoal disease of dogs that poses major health problem worldwide. Farm dogs in rural areas are the companion animals, that not only watch the livestock herds but also guard the house of the owners. Each farmer keeps his companion dog to get all the services. In our study, a total of 450 blood samples of farm dogs from three different ecological zones (Southern, Central and Northern regions of the province; Punjab) of Pakistan, were collected to examine through microscopy and PCR. Examination of thin blood smears revealed an overall prevalence of 12.8% (58/450) of canine babesisal parasites. However, PCR analysis revealed 46.8% (211/450) and 7.3% (33/450) samples positive for B. gibsoni and B. vogeli, respectively. The amplicons of 671 bp and 590 bp were amplified for the detection of B. gibsoni and B. vogeli, respectively through PCR. The results of multivariate analysis showed that the occurrence of canine babesiosis is higher in the Central Punjab and younger age of the dogs, while breed and sex of the host were not significantly associated with the occurrence of the disease. Mixed infection of B. gibsoni and B. vogeli was observed only in 3 dogs each in district Kasur and Rawalpindi. Our study is the first report to observe the occurrence of canine babesiosis in rural dogs in Pakistan through PCR.

Group B streptococcus (GBS) is a common cause of infection in newborns and in early infants. However, GBS infection in an infant older than three months is infrequently reported in the literature. We reported a case of an apparently well six-month-old infant who died of sudden death due to GBS pneumonia, diagnosed at autopsy. The six-month-old, apparently well male infant was brought in dead to the Emergency Department. He underwent medicolegal autopsy four hours after death, as part of an overall sudden unexpected death in infancy investigation (SUDI). Apart from whitish froth oozing out of both nostrils, he appeared to be well-nourished infant without any deformity, syndromic features or obvious suspicious marks of injury externally. Internal examination showed generalized hyperinflated with patchy consolidation of upper and middle lobes of bilateral lung. Multiple matted mesenteric lymphadenopathy were also detected. Blood and lung tissue specimens collected under aseptic technique yielded growth of GBS. Post-mortem histology from consolidated lungs confirmed pneumonic features while mesenteric lymph nodes showed reactive changes inkeeping with underlying infective process. Death was attributed to GBS pneumonia. This case highlights the importance of a detailed autopsy in sudden unexpected death in infancy (SUDI) and the crucial role of post-mortem microbiological study in such cases. Relevant autopsy protocols that need to be employed during microbiological sampling are briefly discussed.

Plants contain numerous bioactive compounds that can be used to develop environmentally-safe insect control agents. Castor oil is a vegetable oil derived from the seeds of castor bean and is widely used as an industrial lubricant and medicinal purgative. In search of an alternative natural insecticide, the objective of this study was to evaluate the larvicidal and adulticidal activities of castor oil against the important dengue vector, Ae. aegypti. Larvicidal and adulticidal bioassays were conducted following the World Health Organization methods. Larvicidal activity was observed at castor oil concentrations of 10, 25, 50, 75, and 100 ppm; larval mortality was checked after 48 h of exposure and the lethal concentration (LC) at LC50 and LC90 were 51.38 and 116.26 ppm, respectively. Adulticidal activity was determined by topical application at the concentrations of 1, 5, 10, 15, and 20 µg/ mg on female mosquitoes and the mortality was checked after 24 h of exposure. The effective adulticidal activity was apparent with the LD50 and LD90 values of 6.03 and 25.07 µg/mg against female mosquitoes. The results indicated that castor oil has potential in the practical control of both immature and adult stages of the mosquito vector.

Planning to control tuberculosis requires identification of dominant strains in the region, transmission patterns and risk factors that are possible by using molecular genotyping techniques. The aim of this study is to determine the transmission of tuberculosis in the northwest of Iran in order to better understand the spread of disease in northwest of Iran. In this study, 194 positive mycobacterium cultivars in northwest of Iran were investigated using exact tandem repeat-variable number tandem repeats (ETR-VNTR) method. The ETR-VNTR method was identified 55 different patterns in 194 isolates, which contained 25 clusters and 30 unique patterns, and the largest cluster had 33 isolates, and discriminatory power of ETRVNTR method was determined 0.9322 in the examined samples. There are strains of Mycobacterium tuberculosis located in the northwest of Iran that infect people, and ETRVNTR method can be used as a first-line method to examine the dynamics of tuberculosis transmission.

Delayed wound healing due to extraneous bacterial contamination, antibacterial resistance and other associated factors are of great concern in dealing patients having chronically infected wound. Medicinal properties of certain maggots of Calliphoridae family are known for its effective wound debridement therapy. The objective of the study was to evaluate the wound healing potential of maggots of Lucilia sericata in an experimentally infected cutaneous wound model in Wistar rat. The study was carried out by using male Wistar rats (n=48) by creating excisional wounds and later contaminated with mixed population of gram positive and gram-negative bacteria. Animals were divided randomly in to four groups with 12 individuals each, being denominated as control, antibiotic treated, maggot treated, and antibiotic plus maggot combination treated group. Ten pre-sterilized maggots were applied per centimetre square wound bed for 24 hours. Different wound kinetics in L. sericata maggot treated wounds revealed significant reduction in wound area with maximum contraction, early elimination of bacterial bioburden as compared to group of infected control and group of rats receiving only antibiotic treatment. The histopathological examination of wounded tissue of maggot treated groups showed early and better epithelialization, collagenation and neovascularization with complete healing of wound in two weeks. The maggot effects on healing when used singly or in combination with antibiotic were recorded to be similar. The results of the present study clearly demonstrate that the maggots of L. sericata possesses a definite antibacterial action along with removal of dead tissues and effectively reduced the bacterial bio-burden in infected wound and induced wound healing quickly.

This study explores the use of a long fragment of the mitochondrial cytochrome oxidase subunit 1 (CO1) marker to elucidate the genetic diversity of Aedes albopictus sampled from urban and forested regions in Peninsular and East Malaysia. A total of 36 samples were collected from 5 localities from which its genetic variability was analysed. 33 distinct mtDNA haplotypes were identified following the amplification and sequencing of the concatenated CO1 gene. The analysed region of the CO1 gene identified substantial levels of genetic diversity among mosquitoes in urban populations and revealed unique genealogical relationships between local isolates as revealed in the haplotype network. This study highlights the reliability of the long CO1 fragment to identify genetic divergence of Aedes albopictus which can be utilized in forthcoming studies.