1.Inhibitive effect of troglitazone on TGF-beta(1) and fibronectin expression in human peritoneal mesothelial cells.
Hong LIU ; You-ming PENG ; Fu-you LIU ; Ying-hong LIU ; Ling-yan LI ; Jun LI ; Xing CHEN
Journal of Central South University(Medical Sciences) 2007;32(3):473-479
OBJECTIVE:
To investigate the effect of the peroxisome proliferator activated receptor-gamma (PPAR-gamma) agonist troglitazone on TGF-beta(1) and fibronectin (Fn) expression in human peritoneal mesothelial cells (HPMCs).
METHODS:
HPMCs were cultured from human omentum by an enzyme digestion method, growing in medium containing 30 mmol/L D-glucose. TGF-beta(1) and Fn expression were measured in HPMCs in the presence and absence of 15 micromol/L troglitazone. The mRNA expressions of PPAR-gamma,TGF-beta(1) and Fn were determined by semi-quantification reverse transcriptive PCR (RT-PCR). The protein of TGF-beta(1) was determined by enzyme-linked immunosorbent assay (ELISA) and proteins of PPAR-gamma and Fn were determined by Western blot.
RESULTS:
The mRNA and protein expression of TGF-beta(1) and Fn were significantly increased in HPMCs stimulated with 30 mmol/L D-glucose compared with the control group with F12 media (P<0.01). Obvious decrease of TGF-beta(1) was found in troglitazone(15 micromol/L) treated group compared with group stimulated with 30 mmol/L D-glucose (P<0.05). Exposure of HPMCs to troglitazone reduced the Fn secretion (P<0.05).
CONCLUSION
Troglitazone reduced the expression of TGF-beta(1) in HPMCs stimulated by 30mmol/L D-glucose, and reduced Fn production. PPAR-gamma agonists may have a specific role in ameliorating the course of progressive peritoneal fibrosis under long-term peritoneal dialysis states.
Blotting, Western
;
Cells, Cultured
;
Chromans
;
pharmacology
;
Dose-Response Relationship, Drug
;
Enzyme-Linked Immunosorbent Assay
;
Epithelial Cells
;
cytology
;
drug effects
;
metabolism
;
Fibronectins
;
biosynthesis
;
genetics
;
Glucose
;
pharmacology
;
Humans
;
PPAR gamma
;
biosynthesis
;
genetics
;
Peritoneum
;
cytology
;
RNA, Messenger
;
biosynthesis
;
genetics
;
Reverse Transcriptase Polymerase Chain Reaction
;
Thiazolidinediones
;
pharmacology
;
Transforming Growth Factor beta1
;
biosynthesis
;
genetics
;
Troglitazone