For the detection of rare phospholipid, phosphatidylethanol (PEt), GC-MS analysis method was adopted for the detection of derivatization products of PEt by N,O-bis (trimethylsilyl) trifluroacetamide (BSTFA). A re-structured molecule derived from PEt, ethyl bis (trimethylsilyl)-phosphate was found from search of Wiley database. This molecule can be used as a marker for PEt analysis. The molecular formula was C8H23O4PSi2 and weight of the formula was 270.09.
Glycerophospholipids/*chemistry ; Mass Fragmentography/*methods ; Support, Non-U.S. Gov't ; Trimethylsilyl Compounds/*chemistry

Humans ; Male ; Middle Aged ; Occupational Diseases ; complications ; Pulmonary Edema ; chemically induced ; Trimethylsilyl Compounds ; poisoning

OBJECTIVETo study the water-soluble non-alkaloid chemical constituents of Corydalis yanhusuo.

METHODThe 80% alcohol extracts of C. yanhusuo passed through DA201 macroporous resin. Eluted fractions were collected and passed though 732 # cation exchange resin. Water eluate was collected, dried and derived with trimethylsilane. Gas Chromatography/Mass Spectrometry and NIST 2005 library were adopted for MS/MS mass spectrogram to infer the compound structure.

RESULTSixteen compounds were tentatively identified from about fifty peaks detected by GC-MS and identified as hydroxyl and carboxyl polar compounds.

CONCLUSIONThese sixteen compounds were found for the first time in C. yanhusuo. The results provide scientific basis for in-depth development of C. yanhusuo.

Corydalis ; chemistry ; Gas Chromatography-Mass Spectrometry ; methods ; Solubility ; Trimethylsilyl Compounds ; chemistry

The objective of study was to establish an animal model with thymic lymphoma in mice induced by intraperitoneal injection of DNA alkylating agent N-methyl-N-nitrosourea (MNU). Male and female mice of the C57BL/6 strain were injected by the intraperitoneal route with MNU solution in a dosage of 50 mg/kg body weight. The injection was repeated at week 8. Following injection of MNU, the general status of mice was observed. All mice were sacrificed for autopsy at the 22nd experimental week. Complete gross examination was performed for detection of tumor masses. The results showed that at the 22nd week, the incidence of thymic lymphoma in MNU-treated animals was 67.5% (27/40). No significant sex difference in the incidence of thymic lymphoma was observed. In conclusions, an animal model with thymic lymphoma in mice can be established by twice intraperitoneal administration of MNU. The biological behavior of the induced tumors resembles to those of human thymic lymphoma derived from thymic T-cells.
Animals ; Female ; Lymphoma ; chemically induced ; Male ; Methylnitrosourea ; adverse effects ; analogs & derivatives ; Mice ; Mice, Inbred C57BL ; Neoplasms, Experimental ; chemically induced ; Thymus Neoplasms ; chemically induced ; Trimethylsilyl Compounds ; adverse effects