The high incidence of intestinal parasites among the Korean people almost same as 40 years ago might be partly interpreted by the fact that they are all fond of raw vegetables cultivated on the farms fertilized with non-treated night soil. The cabbages, onions and radishes were collected from the five areas of Seoul vicinity and the number of parasites attached to those vegetables were investigated to compare with the results obtained 10 years ago by the same methods of sampling and examination as the present survey. The resultant findings were summarized as follows: The eggs of Ascaris, hookworm, Trichostrongylus and whipworm, rhabditoid larvae and filariform larvae of hookworms were detected from those vegetables. Additionally, a number of larvae of free living nematodes and morphologically undetermined eggs were also observed. The maximum number of parasites found from one vegetable were as follows: 16 Ascaris eggs on a cabbage, 27 hookworm eggs on an onion, 8 whipworm eggs on a cabbage, 68 filariform larvae of hookworm on an onion. The onions were highly contaminated by the number of parasites as much as 177.6 including the larvae of free living nematodes, the next being the cabbages with 25.3 and the radishes with 4.1 in average. No notable relationship was observed between the size of vegetables and the number of parasites attached to them. Ascaris eggs were found from 56 percent of all cabbages, 28 percent of the onions and 40 percent of the radishes; whipworm eggs were detected on 40 percent of radishes and the filariform larvae of hookworms were observed from 40 percent of onions examined. It may be concluded that there is no notable change in the number of parasites attached to the vegetables collected from the Seoul vicinity compared to the results investigated by the same method 10 years ago.
parasitology-helminth-nematoda-Ascaris ; hookworm ; Trichuris trichiura ; epidemiology ; vegetable

Usually egg count technique has been employed to know the intensity of infestation of human intestinal helminths and to elucidate the effects of anthelmintics. Comparing the three egg count methods - Stoll's egg dilution method, Beaver's direct smear method and FHK counting board method - the authors reached the following results: As the result of application of the three methods to 75 Ascaris egg positives and 95 Trichocephalus egg positives, in the case of Ascaris eggs, the egg detection rate was 99 per cent by Stoll's method, 97 per cent by Beaver's method and was lowest by FHK method at 79 percent. Meanwhile, in Trichocephalus eggs, Stoll's method revealed 92 percent, Beaver's method 95 percent, and FHK method 95 percent, there being no noticeable differences. With regard to EPG, in counting Ascaris eggs, Stoll's method showed a value closest to the actual number of eggs, and the value revealed by FHK method was smaller than the actual number. As the result of comparing the coefficiencies of variation of the three egg counting methods, both in Ascaris eggs and Trichocephalus eggs, the FHK method showed the smallest, followed by Stoll's method and Beaver's method.
parasitology-helminth-nematoda ; Ascaris lumbricoides ; Trichuris trichiura ; diagnosis

Mebendazole was tested for its efficacy in individual treatment and mass control of Trichuris trichiura infection in Korea. The results were as follows: The efficacy of mebendazole in 430 cases 3 weeks after treatment with mebendazole(100-1,200 mg) was not so satisfactory (15.0-64.0 and 24.6-95.2% in cure and egg reduction rates respectively). But it was observed that the higher the total dose of the drug the better the efficacy. The egg positive rate of the inhabitants in mass control programme by 600 mg mebendazole decreased form 40.0 to 5.6 % during one year, May 1982-May 1983, by repeated blanket mass chemotherapy every 3 months (in conventional regimen), while the decrease was less marked or unrecognizable in 3-month (signle or two divided doses), 6-month, 12-month interval and placebo control groups. From the results, it was concluded that, although mebendazole was not highly effective in individual treatment of T. trichiura infection, it was fairly useful in mass cotrol, by repeated mass chemotherapy every 3 months.
parasitology-helmith-nematoda ; trichuriasis ; Trichuris trichiura ; chemotherapy-mebendazole ; mebendazol

In order to clarify the contamination of vegetable gardens with human intestinal helminths, the soil samples were examined for the presence of eggs and larvae during the period from September 1973 to August l974. When soil samples collected from the vegetable gardens in Taegu were examined, four species of helminth eggs (ascarid, whipworm, hookworm and liver fluke) and a number of unknown eggs were found. Ascarid was found to be highest in the rate of detection(82.5 percent), followed by whipworm. The rates of hookworm, liver fuke and Trichostrongylus species were unexpectedly low. There was no difference between detection rate of vegetable gardens and that of vinyl green houses. However, the average number of helminth eggs detected from the soil of vinyl green houses was higher than the number of vegetable gardens. In the monthly incidence of ascarid eggs, the highest rate was found in September, and the least often was observed in August. While, the peak in the average number of the egg was encountered in August. There was no distinct trend in the monthly incidence of whipworm. However, it is likely that the detection number of whipworm per 100 gm of soil examined was relatively abundant in a period from May to August, and very little from October to April of next year. When the soil of vegetable gardens was divided into superficial, intermediate and deep layers and examined for presence of ascarid egg. The average number of the eggs found of the superficial layer was more abundant than those of the intermediate and deep layers, but the rate for the former was similar with the latter. In the demonstration of larval nematodes from the soil of vegetable gardens and vinyl houses, there found the filariform and rhabditoid larvae of hookworm and the larvae of Trichostrongylus species and unknown one. The majority of the larvae detected was belonged to unknown species, followed by hookworm larvae. The least was found in the rate of Trichostrongylus species. Generally, the filariform larva of hookwom could be detected during the hot season from May to August, but no larva was found in the winter. This suggests that the soil of vegetable gardens and vinyl green houses in Taegu is contaminated with human intestinal helminths.
parasitology-helminth-nematoda-trematoda ; Ascaris lumbricoides ; Trichuris trichiura ; hookworm ; Clonorchis sinensis ; egg ; larva

: A clinical trial of Korean Patent mebendazole was undertaken upon some intestinal helminthic infections in 288 rural Koreans residing in Pochun and Jinyang Gun, from November to December, 1976. They were examined by the cellophane thick smear technique, Stoll's egg counting technique and/or Scotch-tape anal swab before and 3 weeks after the treatment. The administered dose of mebendazole was 600 mg, divided by 100 mg, twice daily for 3 consecutive days disregarding the body weight and the age of the cases. It was confirmed that mebendazole is very effective, broad-spectrum anthelmintic, especially against Ascaris, Ancylostoma, Trichostrongylus and Enterobius infections. Their negative conversion rate and egg reduction rate were respectively; 94.0 and 99.6 per cent in Ascaris, 71.4 and 97.3 per cent in Ancylostoma and 95.0 and 100 per cent in Trichostrongylus. The negative conversion rate in Enterobius was 92.3 per cent. The anthelmintic efficacy against T. trichiura infection was not satisfactory in spite of low worm burden (negative conversion rate, 27.3%, egg reduction rate, 65.5%).
parasitology-helminth-nematoda ; Ascaris lumbricoides ; Ancylostoma duodenale ; Trichostrongylus orientalis ; Trichuris trichiura ; chemotherapy-mebendazole ; Enterobius vermiculariss

A total of 56 subjects with multiple nematode infections with T. trichiura, Ascaris, hookworms and Enterobius were treated with oxantel and pyrantel pamoate mixture in a paratable suspension(50 mg of each per ml). A single dose of 10 mg per kg body weight in each cured 100 per cent of 54 subjects with Ascaris, 97.1 per cent of 35 subjects with hookworms, 77.8 per cent of 36 with Enterobius and 73.2 per cent of 56 subjects with T. trichiura infestation. The mean egg per gram stool reduction rate in T. trichiura infection was 91.9%. Of the 31 subjects infected with Ancylostoma doudenale 96.8% were cured with a single dose and 4 infected with Necator americanus showed a 100% cure rate with a similar daily dose on 3 consecutive days. Side effects were few and mild. There was no clinical or laboratory evidence of drug toxicity. These findings show a single dose of oxantel and pyrantel pamoate mixture to be a highly effective and acceptable treatment for multiple infections with these nematodes.
parasitology-helminth-nematoda ; Trichuris trichiura ; Ascaris lumbricoides ; hookworm ; Enterobius vermicularis ; chemotherapy-oxantel pamoate-pyrantel pamoate

Present trial was carried out to evaluate the anthelmintic efficacy of oxantel/pyrantel tablets against intestinal nematode infections, and to determine the efficacy of oxantel tablels against Trichuris infection. A total of 34 subjects with the mixed infections were treated with oxantel/pyrantel tablets (100 mg/tablet each) in a single dose of 15 mg/kg body weight, and another group of 22 Trichuris infected cases received oxantel pamoate tablets (125 mg/tablet) in a single dose of 15 mg/kg. All stool examinations were done before the treatment and 3 weeks after the treatment. The cellophane thick smear (Kato's technique) and Stoll's dilution egg counting method were employed. The results of the trial were summarized as follows: The cure rates (egg negative conversion rates) and egg reduction rates for oxantel/pyrantel tablets were 85.3 percent and 97 percent in trichuriasis, 100 percent each in ascariasis and ancylostomiasis. The oxantel tablet treated group demonstrated a cure rate of 90.9 percent and an egg reduction rate of 96.3 percent in the treatment of Trichuris. There were no detectable objective and subjective side effects in this trial. Both oxantel/pyrantel and oxantel pamoate tablets were readily accepted and well tolerated.
parasitology-helminth-nematoda ; Enterobius vermicularis ; Ancylostoma duodenale ; Trichuris trichiura ; chemotherapy-oxantel/pyrante ; oxantel pamoate

This study was undertaken to find out the possibility of iron-deficiency anemia by the intestinal blood loss in massive infection cases of T. trichiurus. Among the children of a community in which the whipworm infections were highly prevalent, 7 cases were selected whose E.P.G. of whipworm ova were more than 1,000. The hematological findings and estimated blood loss using (51)Cr were undertaken. The results could be summarized as follows: The E.P.G. and E.P.D. of T. trichiurus ova in studied 7 cases were 10,500-37,200/gm of feces and 0.90-5.14 million/day respectively. Findings of hematological examination were in lower limit of normal in hemoglobin content and hematocrit. And erythrocytes were neither microcytic nor hypochromic. Differential count of leucocytes showed persistently low grade of eosinophilia. Serum iron content measured by modification of Barkan method showed subnormal value in 5 out of 7 cases. Erythrocyte radioactivity half disappearance time(T1/2) and intestinal blood loss were estimated using (51)Cr-tagging erythrocytes. And the results showed that T1/2 were shortened (13-19 days ) in all cases and the blood loss was 0.94-6.54 ml/day which were correlated with worm burden (E.P.D.) significantly. From the above results, it could be concluded that intestinal blood loss of T. trichiurus heavy infection were actually occurred but the amount of losses were easily compensated.
parasitology-helminth-nematoda ; Trichocephalus trichiurus ; Trichuris trichiura ; anemia ; intestine ; hematology ; Cr(51)

A survey on soil samples collected from the vegetable gardens and vinyl houses in the vicinity of Taegu was conducted for the discovery of helminth eggs and larvae from October 1981 to September 1982. The samples collected were examined utilizing the "Commonly Employed" and Kamiya's techniques. The details of comparative features of larval nematodes are taken from the handbook of parasitology by Matsubasyhi et al. (1965). The results were compared with the data obtained from soil samples collected at the same area by Lee(1976). Five kinds of helminth eggs, ascarid, hookworm, liver fluke, Trichostrongylidae, and whipworm and undetermined eggs were found. Of these, the incidence of ascarid eggs was found to be the highest, 70.5 per cent, followed by whipworm and hookworm eggs, 9.9 and 9.2 per cent, respectively. The average number of ascarid eggs per 100 grams of soil was higher in the vinyl houses, compared with the number in the vegetable gardens than in the vinyl houses. By contrast, the number of hookworm eggs was relatively high in the vegetable gardens. In the monthly incidences of helminth eggs, ascarid eggs were found every month, continuing fairly high incidences all the year round. However, hookworm eggs was found for 8 months from February and September, with low monthly incidences between 4.0 and 22.2 per cent, and Trichostrongylidae eggs were found for 6 months from April to September, with lower incidences than those of hookworm. Three kinds of larval nematodes, the filariform and rhabditoid larvae of hookworm, Trichostrongylidae, and undetermined larvae were found in the soil of farms, but the incidences of larvae were quite low. The average number of larvae per 100 grams of soil was 0.05. When the number of eggs per 100 grams of the soil in 1976 were compared with 1982, pronounced reduction of the number was encountered in all helminth eggs. However, the incidences and the average number of larvae per 100 grams of the soil were so low that it was impossible to compare the data between 1976 and 1982.
parasitology-helminth-nematoda ; Ascarid ; hookworm ; Clonorchis sinensis ; Trichostrongylidae ; Trichuris trichiura ; epidemiology

The efficacy of the cellophane thick smear(CTS) technique was reassessed for detecting helminths eggs such as Ascaris lumbricoides, Trichuris trichiura and Clonorchis sinensis, in comparsion with the formalin-ether(F-E) one, with repeated smears per case. From the fecal samples of 411 ROK Army soldiers, a total of 3,288 smears were made and examined; 4 by CTS and 4 by F-E techniques. The expected egg detectability with one, two, three or four smears by each technique was mathemativally calculated. The results were as follows: When the results of all 8 smears per case were integrated, the number of cases eith Ascaris egg were 54(13.1%), Trichuris 125(30.4%) and Clonorchis 33(8.0%). Some other kinds of helminths eggs and protozoan cysts were found from a few cases each. The calculated detectability of Ascaris eggs by CTS technique was fairly high(95.8 - 96.3%) with 1-4 smears, while that by F-E technique was not so high(73.1 - 88.9%). In case of Trchuris eggs, the egg detectability by CTS technique with 1-4 smears was a little lower(62.2-86.4%) than that by F-E technique(73.4 - 91.2%). The detectability of Clonorchis eggs with 1-4 smears by CTS technique was the lowest(44.7 - 60.6%) among the three helminths as well as much lower than that by F-E technique(67.4 - 87.9%).
parasitology-helminth-trematoda-nemaoda ; Ascaris lumbricoides ; Trichuris trichiura ; Clonorchis sinensis ; diagnosis ; formalin ; ether