1.Expressed Sequence Tags of Trichinella spiralis Muscle Stage Larvae.
Hae Kyung PARK ; Seong Won CHANG ; Se Won KANG ; Min Kyoung CHO ; Sun Hee CHOI ; Yeon Chul HONG ; Yong Seok LEE ; Hae Jin JEONG ; Hak Sun YU
The Korean Journal of Parasitology 2008;46(2):59-63
In order to obtain greater insight into the relevant genomic expression patterns of Trichinella spiralis, 992 expressed sequence tags (ESTs) were collected from a cDNA library of T. spiralis muscle stage larvae and assembled into 60 clusters and 385 singletons. Of them, 445 (44.7%) ESTs were annotated to their homologous genes, and small fractions were matched to known genes of nematodes. The annotated ESTs were classified into 25 eukaryotic orthologous groups (KOG). Cytochrome C oxidase (34 clones) was found to be most frequent species.
Animals
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*Expressed Sequence Tags
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Gene Library
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Helminth Proteins/genetics
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Larva/*genetics
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Muscle, Skeletal/*parasitology
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Rats
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Trichinella spiralis/*genetics/isolation & purification
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Trichinosis/*parasitology
2.PCR-RFLP patterns of four isolates of Trichinella for rDNA ITS1 region.
Hye Soo KWON ; Myung Sook CHUNG ; Kyoung Hwan JOO
The Korean Journal of Parasitology 2001;39(1):43-48
We have studied the genetic differences among four isolates of Trichinella including a new strain of Trichinella spiralis (ISS 623) recently found from a human case who took a badger in Korea. Because they have a different host origin and came from geographically separated regions, we supposed the genetic pattern of the isolates might be different as had been previously reported. It was analysed by PCR-RFLP analysis of the rDNA repeat that can readily distinguish a species or strain from others. Isolated genomic DNA of each isolate of Trichinella larvae was amplified with ITS1 specific primers and digested with restriction endonucleases. The PCR product of ITS1 was confirmed using Southern blot analysis to be a 910 bp fragment. The restriction fragments of each isolate had variable patterns when it was digested with Rsa 1 only. According to the RFLP patterns, the estimated genetic divergence between each isolate was different. In conclusion, four isolates of Trichinella including a new strain of T. spiralis obtained from a Korean patient may have genetic differences in the ITS1 region and the Shanghai isolate was genetically more similar to the Japanese unknown isolate than others in the ITS1 region.
Animals
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*DNA, Helminth
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*DNA, Ribosomal
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Human
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Repetitive Sequences, Nucleic Acid
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Trichinella spiralis/*genetics/isolation & purification
3.Molecular Identification of a Trichinella Isolate from a Naturally Infected Pig in Tibet, China.
Ling Zhao LI ; Zhong Quan WANG ; Peng JIANG ; Xi ZHANG ; Hui Jun REN ; Jing CUI
The Korean Journal of Parasitology 2011;49(4):381-384
The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from Tibet Trichinella isolate from a naturally infected pig. The Tibet Trichinella isolate was also identified by PCR amplification of the 5S ribosomal DNA intergenic spacer region (5S ISR) and mitochondrial large-subunit ribosomal RNA (mt-lsrDNA) gene sequences. The results showed that 2 DNA fragments (749 bp and 445 bp) of the Tibet Trichinella isolate were identical to that of the reference isolates of T. spiralis. The Tibet Trichinella isolate might be classifiable to T. spiralis. This is the first report on T. spiralis in southwestern China.
Animals
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DNA, Helminth/chemistry/genetics
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DNA, Mitochondrial/chemistry/genetics
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DNA, Ribosomal/chemistry/genetics
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DNA, Ribosomal Spacer/genetics
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Genotype
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Humans
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Multiplex Polymerase Chain Reaction
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RNA, Ribosomal, 5S/genetics
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Sequence Analysis, DNA
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Swine
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Swine Diseases/*parasitology
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Tibet
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Trichinella spiralis/*classification/genetics/isolation & purification
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Trichinellosis/parasitology/*veterinary
4.An Outbreak of Trichinosis with Molecular Identification of Trichinella sp. in Vietnam.
Nguyen Van DE ; Nguyen Vu TRUNG ; Nguyen Hong HA ; Vu Thi NGA ; Nguyen Minh HA ; Pham Thanh THUY ; Le Van DUYET ; Jong Yil CHAI
The Korean Journal of Parasitology 2012;50(4):339-343
The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment. Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of fever, muscle pain, difficult moving, edema, difficult swallowing, and difficult breathing. ELISA revealed all (6/6) positive reactions against Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the muscle. The larvae detected in the patients were identified as T. spiralis (Vietnamese strain) by the molecular analysis of the mitochondrial cytochrome c oxidase subunit III (cox3) gene.
Adult
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Animals
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Antigens, Helminth/analysis/immunology
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*Disease Outbreaks
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Electron Transport Complex IV/genetics
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Enzyme-Linked Immunosorbent Assay
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Female
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Humans
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Larva
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Male
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Meat/*parasitology
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Mitochondria/genetics
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Muscles/parasitology/pathology
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Swine
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Trichinella spiralis/genetics/immunology/*isolation & purification
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Trichinellosis/*epidemiology/parasitology/pathology
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Vietnam/epidemiology