This study aims to provide detailed information on the role of the following social determinants (i.e. education, occupation, place of residence, marital and economic status) in the acquisition of HIV knowledge among women of childbearing age (15-49 years old). The study uses secondary data from the 2012 Indonesian Demographic and Health Survey (IDHS). The IDHS used a quantitative analytical approach with a cross-sectional study design and multi-stage stratified sampling. The sample included 1,335 women aged 15 to 49 years old living in South Sumatra Province, Indonesia. Descriptive analysis, ANOVA, and t-test and multiple logistic regressions were performed in this study. The results show one out of two women have little HIV knowledge. The more highly educated women were positively associated with a greater degree of knowledge related to comprehensive knowledge related to HIV (AOR: 3.7, 2.2-6.2, p.<0.05), HIV prevention (AOR:16.6, 1.8-153.9, p <0.05), transmission from mother to child (AOR:11.1, 3.3-36.9, p <0.05), HIV misconceptions (AOR:5.1, 1.1-23.1, p <0.05) and HIV services (AOR:5.7, 2.8-11.4, p < 0.05). In addition, the women lived in urban areas had more knowledge related to HIV prevention and transmission (AOR: 3.36, 1.8-6.2, p <0.05), and knowledge about mother-to-child of HIV transmission (AOR: 3.2, 1.8-5.5, p <0.05) compared to those who lived in rural areas. To conclude, education level was found to be the dominant factor associated with knowledge of HIV across all categories of knowledge after controlling by other determinants, age, marital status, working status and residence. HIV awareness and comprehensive HIV information among women who are considered to be in a low-risk group for HIV infection should be integrated with formal education as well as in maternal health services, particularly in rural areas.

Purpose: Indonesia, a high populous and the second-highest country in epidemicity of hepatitis B in South-East Asia require maintaining its capacity of monovalent hepatitis B production to keep up with both the national immunization program and global needs. To keep the sustainability of the vaccine, a new bulk is needed to be made available. This study aims to evaluate the immunogenicity and safety of Bio Farma newly formulated recombinant hepatitis B vaccines, which came from different sources of bulk, compared to the already registered hepatitis B vaccine. Materials and Methods: An experimental, randomized, double-blind, cohort intervention phase II clinical trial was conducted on three recombinant hepatitis B vaccines from different bulk sources, with Bio Farma registered hepatitis B vaccine as the control group. A total of 536 participants around age 10 to 40 years old were thricely vaccinated with twice serological assessments. The subject’s safety was monitored for 28 days after each vaccination. Results: Of 536 enrolled participants, 521 finished the vaccination and serology assessments. The investigational products were proven not to be inferior to the control. All vaccines were well tolerated. No differences in rates of local and systemic reactions were seen between the investigational products and control. No serious adverse event was found to be related to the investigational vaccines. Conclusion Investigational vaccines are shown to be equally immunogenic and safe as the control vaccine.

This research was conducted to examine the effect of cholecalciferol on fasting blood glucose (FBG), adipocyte diameter and glucose transporter (GLUT) 4 expression in adipocytes of diabetic rats. Nineteen male Wistar strain diabetic rats were divided into 4 groups (K, X1, X2 and X3). Cholecalciferol was administered in the amount of 6.25 μg/kg in X1, 12.5 μg/kg in X2 and 25 μg/kg in X3 per orem, once daily for 14 days. Group K received the placebo. There were no significant differences in FBG (p=0.199) and adipocyte diameter (p=0.218) between groups but there were significant differences in the expression of GLUT4 between control and treatment groups. Thus, cholecalciferol can increase GLUT4 expression in adipocyte without altering FBG and adipocyte diameter of diabetic rats.
Cholecalciferol ; Adipocytes

Objective: To describe the prevalence of antiphospholipid antibodies in coronavirus disease-19 (COVID-19) and to find potential associations between antiphospholipid antibody positivity and clinical outcomes. Methods: From September to November 2020, clinical and laboratory data were collected from 50 COVID-19 patients hospitalized at Saiful Anwar General Hospital in Malang, Indonesia. Antiphospholipid antibodies were measured by finding IgM anti-β2 glycoprotein, lupus anticoagulant, and IgM/IgG anticardiolipin. Clinical characteristics, thrombotic events, ICU admission, and mortality during hospitalization were recorded. Disease severity was defined by the Guidelines for the Prevention and Control of COVID-19, Indonesia. Results: Among 50 patients, 5 patients (10.0%) were positive for antiphospholipid antibodies: 4 patients (80.0%) had IgM anti-β2 glycoprotein and 1 patient had IgG anti-cardiolipin (20.0%) and IgM anti-cardiolipin (20.0%), none of lupus anticoagulant was detected. Antiphospholipid antibodies were associated with anosmia (OR 8.1; 95% CI 1.1-57.9; P=0.018), nausea and vomiting (OR 12.4; 95% CI 1.2-122.6; P=0.010), diarrhea (OR 9.8; 95% CI 1.3-70.9; P=0.010), cardiovascular disease (OR 1.4; 95% CI 1.0-1.9; P=0.001), chronic kidney disease (OR 12.0; 95% CI 1.6-90.1; P=0.05), acute coronary syndrome (OR 29.3; 95% CI 2.0-423.7; P=0.001), moderate (OR 0.11; 95% CI 0.01-1.10; P=0.031) and severe (OR 18.5; 95% CI 1.8-188.4; P=0.002) disease severity, and in-hospital mortality (OR 8.1; 95% CI 1.1-57.9; P=0.018). However, there is no correlation between the presence of antiphospholipid antibody and ICU admission. Conclusions: In summary, the prevalence of antiphospholipid antibodies in COVID-19 patients is low, mainly against IgM anticardiolipin, and is associated with an acute coronary syndrome, gastrointestinal manifestations, moderate and severe disease severity, and increased risk of mortality.

Aims: This study aims to produce Achromobacter biosurfactant in nutrient-rich and nutrient-limited media. Methodology and results: This study conducted fermentation on nutrient-rich and nutrient-limited media using a minimal salt medium (MSM). Dextrose and sodium citrate were used as sole carbon supplemented with 0.5% yeast extract for nutrient-rich media, while nutrient-limited media used molasses and rice straw hydrolysate (RSH) at variations of concentrations of 100 ppm and 200 ppm. The research was performed over 120 h and evaluated from growth response, surface tension and emulsification activity. The study revealed that the best surface tension value was when 2% (w/v) sodium citrate was used as C-source and 0.5% (w/v) yeast extract as N-source, after 72 h upon incubation at 30 °C/120 rpm having 45.45 ± 2.19 mN/m with emulsification activity 24.54 ± 3.42%. Whereas the best result of the nutrient-limited medium was obtained by RSH at a concentration of 200 ppm having 48.86 ± 5.36 mN/m. Conclusion, significance and impact of study The experiment showed that nutrient-limited medium from rice straw hydrolysate could compete with the nutrient-rich medium. The use of rice straw will contribute to the reduction of biosurfactant production costs and valorisation of agricultural waste.
Achromobacter denitrificans ; Surface-Active Agents

To assess healthcare workers' knowledge of novel coronavirus disease 2019 (COVID-19) in the early phase of the outbreak in Indonesia. Methods: A cross-sectional survey was conducted in 12 hospitals in Indonesia from March 6 to March 25, 2020. Healthcare workers' knowledge on COVID-19 was assessed, and demographic data, workplace characteristics, and medical professional characteristics as well as the current local situation of COVID-19 were collected. To characterize determinants associated with knowledge, a logistic regression analysis was employed. Results: Out of 288 healthcare workers who completed the interview-assisted questionnaire, 149 (51.7%) respondents had a good knowledge. Nurses and other types of healthcare workers had lower odds of having good knowledge compared to doctors: adjusted odds ratio (aOR): 0.38; 95% CI: 0.20-0.72 and aOR: 0.31; 95% CI: 0.13-0.73, respectively. Compared to healthcare workers who had medical practice experience less than 5 years, those who had worked for more than 10 years had lower knowledge (aOR: 0.43; 95% CI: 0.20-0.90). Healthcare workers who worked in the infection department had higher knowledge compared to those in the emergency room (aOR: 14.33; 95% CI: 3.67-55.88). Conclusions: The knowledge of COVID-19 among surveyed healthcare workers was relatively low. The COVID-19 response in Indonesia will require further education and enhancement of the capacity of healthcare workers in the emergency room where COVID-19 patients may be treated the earliest.