1.Protective effect of prostaglandin E1 against brain injury induced by hyperoxia in neonatal rats.
Shan YANG ; You-Chen ZHANG ; Hui-Wen LI ; Zheng-Yong JIN
Chinese Journal of Contemporary Pediatrics 2018;20(3):230-235
OBJECTIVETo investigate the protective effect of prostaglandin E1 (PGE-1) against brain injury induced by hyperoxia in neonatal rats and observe the changes in the expression of glucose-regulated protein 78 (GRP78) and cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP), and to provide a theoretical basis for the clinical application of PGE-1 in the treatment of neonatal brain injury induced by hyperoxia.
METHODSSixty neonatal Wistar rats were randomly divided into air control group, hyperoxic brain injury model group, and hyperoxic brain injury+PGE-1 group. All rats except those in the air control group were treated to establish a hyperoxic brain injury model. From the first day of modeling, the rats in the hyperoxia brain injury+PGE-1 group were intraperitoneally injected with PGE-1 2 μg/kg daily for 7 consecutive days, while the other two groups were treated with normal saline instead. The water content of brain tissue was measured; the pathological changes of brain tissue were evaluated by hematoxylin-eosin staining; the apoptosis of brain cells was assessed by nuclear staining combined with TUNEL staining; the protein expression of GRP78 and CHOP in brain tissue was measured by Western blot.
RESULTSThe water content of brain tissue in the hyperoxic brain injury model group was significantly higher than that in the hyperoxic brain injury+PGE-1 group and air control group (P<0.05); the water content of brain tissue in the hyperoxic brain injury+PGE-1 group was significantly higher than that in the air control group (P<0.05). The pathological section of brain tissue showed inflammatory cell infiltration and mild cerebrovascular edema in the brain parenchyma in the hyperoxic brain injury model group; the periparenchymal inflammation and edema in the hyperoxic brain injury+PGE-1 group were milder than those in the hyperoxic brain injury model group. The apoptosis index of brain tissue in the hyperoxic brain injury model group was significantly higher than that in the hyperoxic brain injury+PGE-1 group and air control group (P<0.05); the apoptosis index of brain tissue in the hyperoxic brain injury+PGE-1 group was significantly higher than that in the air control group (P<0.05). The protein expression of GRP78 and CHOP in brain tissue was significantly higher in the hyperoxic brain injury model group than in the hyperoxic brain injury+PGE-1 group and air control group (P<0.05); the protein expression of GRP78 and CHOP was significantly higher in the hyperoxic brain injury+PGE-1 group than in the air control group (P<0.05).
CONCLUSIONSPGE-1 has a protective effect against hyperoxia-induced brain injury in neonatal rats, which may be related to the inhibition of cell apoptosis by down-regulating the expression of GRP78 and CHOP.
Alprostadil ; therapeutic use ; Animals ; Animals, Newborn ; Apoptosis ; drug effects ; Brain ; pathology ; Brain Injuries ; metabolism ; pathology ; prevention & control ; Heat-Shock Proteins ; analysis ; Hyperoxia ; complications ; Neuroprotective Agents ; therapeutic use ; Rats ; Rats, Wistar ; Transcription Factor CHOP ; analysis
2.The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability.
Kentaro ITO ; Akinori TAKAHASHI ; Masahiro MORITA ; Toru SUZUKI ; Tadashi YAMAMOTO
Protein & Cell 2011;2(9):755-763
The human CCR4-NOT deadenylase complex consists of at least nine enzymatic and non-enzymatic subunits. Accumulating evidence suggests that the non-enzymatic subunits are involved in the regulation of mRNA deadenylation, although their precise roles remain to be established. In this study, we addressed the function of the CNOT1 subunit by depleting its expression in HeLa cells. Flow cytometric analysis revealed that the sub G(1) fraction was increased in CNOT1-depleted cells. Virtually, the same level of the sub G1 fraction was seen when cells were treated with a mixture of siRNAs targeted against all enzymatic subunits, suggesting that CNOT1 depletion induces apoptosis by destroying the CCR4-NOT-associated deadenylase activity. Further analysis revealed that CNOT1 depletion leads to a reduction in the amount of other CCR4-NOT subunits. Importantly, the specific activity of the CNOT6L immunoprecipitates-associated deadenylase from CNOT1-depleted cells was less than that from control cells. The formation of P-bodies, where mRNA decay is reported to take place, was largely suppressed in CNOT1-depleted cells. Therefore, CNOT1 has an important role in exhibiting enzymatic activity of the CCR4-NOT complex, and thus is critical in control of mRNA deadenylation and mRNA decay. We further showed that CNOT1 depletion enhanced CHOP mRNA levels and activated caspase-4, which is associated with endoplasmic reticulum ER stress-induced apoptosis. Taken together, CNOT1 depletion structurally and functionally deteriorates the CCR4-NOTcomplex and induces stabilization of mRNAs, which results in the increment of translation causing ER stress-mediated apoptosis. We conclude that CNOT1 contributes to cell viability by securing the activity of the CCR4-NOT deadenylase.
Apoptosis
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Caspases, Initiator
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genetics
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metabolism
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Cell Survival
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Endoplasmic Reticulum
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enzymology
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Enzyme Activation
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Flow Cytometry
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HEK293 Cells
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HeLa Cells
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Humans
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Protein Subunits
;
genetics
;
metabolism
;
RNA Stability
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RNA, Messenger
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analysis
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RNA, Small Interfering
;
genetics
;
metabolism
;
Ribonucleases
;
metabolism
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Stress, Physiological
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Transcription Factor CHOP
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genetics
;
metabolism
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Transcription Factors
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genetics
;
metabolism
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Transfection
3.Effects of erythropoietin on cardiomyocyte apoptosis and endoplasmic reticulum stress-related proteins in neonatal rats with asphyxia.
Chinese Journal of Contemporary Pediatrics 2013;15(10):890-895
OBJECTIVETo study the effects of erythropoietin (EPO) on cardiomyocyte apoptosis and endoplasmic reticulum stress (ERS)-related proteins, glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP), in neonatal rats with asphyxia.
METHODSA total of 120 newborn Sprague-Dawley rats (7 days old) were randomly divided into sham-operated (n=40), asphyxia (n=40) and EPO-treated asphyxia groups (n=40). A neonatal rat model of normobaric asphyxia was established in the asphyxia and EPO-treated asphyxia groups. The rats in the EPO-treated asphyxia group received intraperitoneal injection of recombinant human erythropoietin (500 U/mL) immediately after the model was established, while the other two groups received the same volume of normal saline (0.9%). Heart blood and myocardial tissue samples were collected from 8 rats in each group at 2, 6, 12, 24 or 48 hours after the model was established. Serum creatine kinase (CK) and lactate dehydrogenase (LDH) levels were measured; cardiomyocyte apoptosis was evaluated, and expression of myocardial GRP78 and CHOP was measured.
RESULTSCompared with the sham-operated and EPO-treated asphyxia groups, the asphyxia group had significantly increased serum CK and LDH levels, number of apoptotic cells, and expression of myocardial GRP78 and CHOP at each time point (P<0.01), and all the indices were significantly higher in the EPO-treated asphyxia group than in the sham-operated group (P<0.01). At 24 hours after asphyxia, the expression of myocardial CHOP was positively correlated with the myocardial apoptosis index (r=0.944, P<0.01).
CONCLUSIONSEPO exerts a protective effect on the myocardium of neonatal rats with hypoxic-ischemic injury by regulating ERS-related proteins GRP78 and CHOP and reducing cardiomyocyte apoptosis.
Animals ; Animals, Newborn ; Apoptosis ; drug effects ; Asphyxia Neonatorum ; drug therapy ; pathology ; Creatine Kinase ; blood ; Endoplasmic Reticulum Stress ; physiology ; Erythropoietin ; pharmacology ; Heat-Shock Proteins ; analysis ; L-Lactate Dehydrogenase ; blood ; Myocytes, Cardiac ; drug effects ; pathology ; Rats ; Rats, Sprague-Dawley ; Transcription Factor CHOP ; analysis
4.Paxilline enhances TRAIL-mediated apoptosis of glioma cells via modulation of c-FLIP, survivin and DR5.
You Jung KANG ; In Young KIM ; Eun Hee KIM ; Mi Jin YOON ; Seung U KIM ; Taeg Kyu KWON ; Kyeong Sook CHOI
Experimental & Molecular Medicine 2011;43(1):24-34
Tumor necrosis factor-related apoptosis-induced ligand (TRAIL) induces apoptosis selectively in cancer cells while sparing normal cells. However, many cancer cells are resistant to TRAIL-induced cell death. Here, we report that paxilline, an indole alkaloid from Penicillium paxilli, can sensitize various glioma cells to TRAIL-mediated apoptosis. While treatment with TRAIL alone caused partial processing of caspase-3 to its p20 intermediate in TRAIL-resistant glioma cell lines, co-treatment with TRAIL and subtoxic doses of paxilline caused complete processing of caspase-3 into its active subunits. Paxilline treatment markedly upregulated DR5, a receptor of TRAIL, through a CHOP/GADD153-mediated process. In addition, paxilline treatment markedly downregulated the protein levels of the short form of the cellular FLICE-inhibitory protein (c-FLIPS) and the caspase inhibitor, survivin, through proteasome-mediated degradation. Taken together, these results show that paxilline effectively sensitizes glioma cells to TRAIL-mediated apoptosis by modulating multiple components of the death receptor-mediated apoptotic pathway. Interestingly, paxilline/TRAIL co-treatment did not induce apoptosis in normal astrocytes, nor did it affect the protein levels of CHOP, DR5 or survivin in these cells. Thus, combined treatment regimens involving paxilline and TRAIL may offer an attractive strategy for safely treating resistant gliomas.
Antineoplastic Agents/*pharmacology
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Apoptosis/*drug effects
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Astrocytes/metabolism
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CASP8 and FADD-Like Apoptosis Regulating Protein/genetics/*metabolism
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Caspase 3/metabolism
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Cell Line, Tumor
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Drug Discovery
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Flow Cytometry
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Glioma/*metabolism/pathology
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Humans
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Indoles/*pharmacology
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Inhibitor of Apoptosis Proteins/metabolism
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RNA, Small Interfering
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Receptors, TNF-Related Apoptosis-Inducing Ligand/genetics/metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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TNF-Related Apoptosis-Inducing Ligand/metabolism/*pharmacology
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Transcription Factor CHOP/analysis
5.Cytogenetics and molecular biology of liposarcoma.
Hua XIANG ; Jian WANG ; Xiong-zeng ZHU
Chinese Journal of Pathology 2003;32(2):165-167
CCAAT-Enhancer-Binding Proteins
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genetics
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Cytogenetic Analysis
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Cytogenetics
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methods
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Extremities
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Humans
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Liposarcoma
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etiology
;
genetics
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Liposarcoma, Myxoid
;
etiology
;
genetics
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Molecular Biology
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methods
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Oncogene Proteins, Fusion
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genetics
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RNA-Binding Protein FUS
;
genetics
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Retroperitoneal Neoplasms
;
etiology
;
genetics
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Ring Chromosomes
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Soft Tissue Neoplasms
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etiology
;
genetics
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Transcription Factor CHOP
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Translocation, Genetic
6.Detection of chromosomal translocation in fresh samples of myxoid/round cell liposarcoma by long-distance polymerase chain reaction.
Hua XIANG ; Jian WANG ; Masanori HISAOKA ; Xiong-zeng ZHU
Chinese Journal of Pathology 2007;36(6):412-413
Adult
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Base Sequence
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DNA, Neoplasm
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genetics
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Exons
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Female
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Humans
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Liposarcoma
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genetics
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Liposarcoma, Myxoid
;
genetics
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Male
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Middle Aged
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Molecular Sequence Data
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Oncogene Proteins, Fusion
;
genetics
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Polymerase Chain Reaction
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methods
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RNA-Binding Protein EWS
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genetics
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RNA-Binding Protein FUS
;
genetics
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Sequence Analysis, DNA
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Transcription Factor CHOP
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genetics
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Translocation, Genetic