The study used 300 E. coli strains isolated from specimens: stool (150), urine (40), blood (30), billiary fluid (40), pus (40) to find sensitivity and specificity of multiplex PCR in diagnosis of E. coli strains causing diarrhea and distribution of E. coli which have ability causing diarrhea in these specimens. DNA was extracted from E. coli control strains, reference strains belong to Enterobacteiaceaer and E. coli strains from Karolinska Institute (Sweden) and then carrying out multiplex (8 primer pairs) PCR reaction.
Diarrhea ; diagnosis ; Escherichia coli