The samples obtained from the 25 healthy adults donors, from19 to 50 years old of age, 18 men and 7 woman. Different antibodies match different methods. Standard method: CD3 (FITC) / CD16, CD56 (PE) / CD45 (PerCP) / CD19 (APC) mixture of antibodies. Estimate method: 3 colors use CD4 (FITC) / CD3 (PE) / CD8 (PerCP) mixture of antibodies and 4 colors use CD3 (FITC) / CD8 (PE) / CD45 (PerCD) / CD4 (APC) mixture of antibodies. Results: standard method - estimate method 3 colors: r = 0,85; % NK (adjust) = 1,99 x % NK (estimated) + 2,31; standard method - estimate method 4 colors: r = 0,85; % NK (adjust) = 1,63 x % NK (estimated) + 4,53
Tissue Donors ; methods ; Reference Standards

The research aimed to establish the maximum time elapsed between collection and analysis of samples. The blood samples were stored at temperature varying from 200C to 280C. Studied on 32 subjects, all samples were marked by the monoclonal antibodies. Each sample had been analyzed 4 times: within 6 hours, 24 hours, 48 hours and 72 hours after drawing. Epi-info 2000 had been used for statistical analysis the results. Results showed that: the maximum elapse of time for the CD4 was 72 hours, for the CD8 was 24 hours and for the lympho and the WBC's as little time as possible, because the results were very different every second time
Blood ; cells ; Temperature

The objective of study was to establish reference ranged for the lymphocyte subset (TCD3, TCD4, TCD8, B, NK) in peripheral blood of adult donors. This reference is used like target values for patients examinated in HCM city’s Pasteur Institute and then the results were compared with those obtained in Iranian healthy adults. Healthy person’s blood was processed with EDTA anticoagulation then incubated with 2 groups of antibodies from blood donors contained in 2 diverse tubes CD3/CD8/CD45/CD4 and CD3/CD16,56/CD45/CD19. Then through FASC apparatus, the samples were analyzed, and TCD3, TCD4, TCD8, B, NK were determined in each sample diversely.
Tissue Donors ; Adult ; Lymphocyte Subsets