The samples obtained from the 25 healthy adults donors, from19 to 50 years old of age, 18 men and 7 woman. Different antibodies match different methods. Standard method: CD3 (FITC) / CD16, CD56 (PE) / CD45 (PerCP) / CD19 (APC) mixture of antibodies. Estimate method: 3 colors use CD4 (FITC) / CD3 (PE) / CD8 (PerCP) mixture of antibodies and 4 colors use CD3 (FITC) / CD8 (PE) / CD45 (PerCD) / CD4 (APC) mixture of antibodies. Results: standard method - estimate method 3 colors: r = 0,85; % NK (adjust) = 1,99 x % NK (estimated) + 2,31; standard method - estimate method 4 colors: r = 0,85; % NK (adjust) = 1,63 x % NK (estimated) + 4,53
Tissue Donors ; methods ; Reference Standards

The research aimed to establish the maximum time elapsed between collection and analysis of samples. The blood samples were stored at temperature varying from 200C to 280C. Studied on 32 subjects, all samples were marked by the monoclonal antibodies. Each sample had been analyzed 4 times: within 6 hours, 24 hours, 48 hours and 72 hours after drawing. Epi-info 2000 had been used for statistical analysis the results. Results showed that: the maximum elapse of time for the CD4 was 72 hours, for the CD8 was 24 hours and for the lympho and the WBC's as little time as possible, because the results were very different every second time
Blood ; cells ; Temperature

Studying total number of 1,404 HIV infected persons, including 716 AIDS manifestation and 245 AIDS deaths in Dong Thap province from 3/2003 - 4/2003 showed that: the new infectious number of HIV in this province has been increasing quickly; Dong Thap ranks tenth in ten provinces having highest HIV/100,000 people rate (89.53%). Although, the HIV/AIDS epidemic in Dong Thap is still in concentrated period but the distribution of HIV infection with 3% children and 2.8% pregnant women is a worried problem. Moreover, quantity and risk behaviors of HIV/AIDS infection in commercial sex workers and injecting drug users are also worried problems. Basing on these results, Dong Thap should mobilize all potential resources to implement quickly the measures including unnamed test counseling and activities which prevent mother-baby HIV spread helping reduce damaging effect in high risk people
HIV,Acquired Immunodeficiency Syndrome, Sex, Substance-Related Disorders

38 blood samples were divided into 2 groups – normal sample (with 3 groups of cells seen obviously; lymphocyte, mon, gronulocyte on FSC, SSC windows) and abnormal sample group (without these 3 groups of cells).
Antibodies ; cells ; Blood

The objective of study was to establish reference ranged for the lymphocyte subset (TCD3, TCD4, TCD8, B, NK) in peripheral blood of adult donors. This reference is used like target values for patients examinated in HCM city’s Pasteur Institute and then the results were compared with those obtained in Iranian healthy adults. Healthy person’s blood was processed with EDTA anticoagulation then incubated with 2 groups of antibodies from blood donors contained in 2 diverse tubes CD3/CD8/CD45/CD4 and CD3/CD16,56/CD45/CD19. Then through FASC apparatus, the samples were analyzed, and TCD3, TCD4, TCD8, B, NK were determined in each sample diversely.
Tissue Donors ; Adult ; Lymphocyte Subsets

In 6 simples of Nga Truat. 2 simples of Dia Lien, 2 samples of Cu Coc collected in Nghia Trai village. There was no-orgam chlorine pollutants; but in 1 of 9 samples of camomille containing cypermethrine residue exceeded the normal standard
Insecticides ; Medicine, Traditional ; Pharmaceutical Preparations

Study was conducted on 1048 households with 6068 persons in A Luoi district, Thua Thien Hue province, where herbicides were sprayed heavily in war. The relationship between the quantity of herbicide sprayed in war and the severity of environmental pollution at present was recorded. Epidemiological relationship between the pollution of the enviroment and abnormal reproductivity and congenital defects, which were 1.5-4 times higher after the spray versus before the spray with diverse levels among 3 communes exposed to the spray. The most common abnormal reproductivity was spontaneous abortion, then premature delivery with died infants and congenital defects, the stillbirth and hydratiform mole were with lower rate. Dioxine was transported from the enviroment to human body through the exposed food chain such as chicken, fish, duck
Reproduction ; Dioxins ; Herbicides

213 adult subjects, children of veterans exposed to herbicides sprayed during Ranch Hand Operation (in 1961-1971 war period) and 210 non-exposed subjects were investigated by usual lumbar X-ray. The images showed that spina bifida occulta incidence in exposed group was 1.5 times higher than non-exposed group. Among male subjects, in exposed group, the incidence was 1.7 times higher than non-exposed one, while among female subjects, no difference was recorded. Simple single bifida was 51.6% in exposed group and 41.9% in non-exposed. Multiple vertebra of combinated bifida was 7.9% and 2.4% respectively. The common sites of bifida were L5, S1, S2, S3, among them the site of S1 was most common
Spina Bifida Occulta ; Herbicides ; Child

Study on hepatoprotective effects of lingzhi extract on CCl4-induced hepatic lesion, lingzhi formulation produced by OPC Company which reached standards at basic level. Lingzhi dry powder was prepared with distilled water to make liquid extract with concentrations of 15% (15g dry extract/100ml water), 30% and 75%. These liquid extracts were used to test hepatoprotective effects. With low dose CCl4 (0,25%) induced hepatic toxication, 15%, 30% and 75% lingzhi extracts showed hepatoprotective effect. However, with high dose CCl4 (1%), only 30% lingzhi extract showed clear and stable hepatoprotective effects. Experimental trials on hepatic diseases as well as on hepatoprotective medications should provide necessary information on physiopathological characteristics and medicinal therapies
Drugs, Chinese Herbal ; Liver

Study on 100 gastroduodenal ulcer patients treated at Can Tho General Hospital between March and December 2001. Results: the prevalence of gastric ulcer was high (58%), followed by duodenal ulcer (39%) and the prevalence of gastroduodenal ulcer was low (3%). The rate of H.pylori positive was 66.66% in duodenal ulcer patients, 55.17% in gastric ulcer patients. The rate of H.pylori infection was 33.33% in gastroduodenal ulcer patients. H.pylori was still sensitive with amoxicillin (97.29%), tetracycline (94.6%), clarythromycine and erythromycin (72.97%), but was highly resistant with metronidazole (91%).
Helicobacter pylori ; Peptic Ulcer ; Epidemiology