The initial investigation of pesticides using cultivation of medical plants in ThietTru, BinhMinh, KhoaiChau, HungYen was reported. Almost pesticides in common use for cultivation of medicinal plants there were recorded and classified. The results of investigation showed that they were often used extensively and in a wrong way. although the Organo-chlorine pesticides (except endosulfan) were no longer in use but at least one prohibited pesticide (methamidophos) were still illegally overused. Further investigation in broader areas for some other localities should be undertaken for systematical evaluation of pesticides use in the cultivation of medicinal plants, and especially its impact. Residual pesticides in herbal medicines should be determined as well, since then, some active measure of management and method of control will be proposed in order to strengthen the quality assurance of this precious resource of medicines.
chemistry ; Pharmaceutical Preparations ; Plants, Medicinal ; Medicine, Traditional

Two-stage carcinogenesis has been widely used as a method for evaluating precancerous lesion on animal experiments. Here we used diethylnitrosamine (DEN), a tumor trigger, and carbon tetrachloride (CCl4), a tumor promoter, to mimic the first two stages of hepatic carcinogenesis. Single intra-peritoneal injection of DEN 1% in combination with repeated intra-peritoneal injection of CCl4 4% for 16 weeks led to the formation of hyperplasia nodules on mouse livers. Microscopic examination also revealed dramatic changes in hepatocellular morphology and architecture: increased cell density and proliferation, abnormalities in cell division and nucleus structure. These changes were not observed in animal treated with NaCI or DEN alone. Repeated injection of CCl4 alone led to alterations on microscopic pictures but did not cause hyperplasia nodules. Our results indicate that DEN in combination of CCl4 causes abnormal proliferation of hepatic cells and evokes hepatic hyperplasia
Hepatocytes ; Cell Proliferation ; Carbon Tetrachloride

It’s very important to identify pathological anatomic features in the treatment and prognosis of testicular cancer. In this study, 112 patients who were diagnosed by clinical and paraclinical symptoms were classified according to age, location, and severity. Their samples then were stained by PSA test. Results: 49% were germ cell tumors of scrotum, 9% were none germ cell tumors, and 42% were dystopic germ cell tumors. Base on pathological anatomic features (stage, location) we can apply different methods of treatment to gain better outcomes
Testicular Neoplasms ; Pathological Conditions, Anatomical ; Prognosis ; diagnosis

The survey last from Dec 9th to Dec 18 th 2002 with total number of 1558 HIV infected persons, including 344 AIDS manifestation; 160 AIDS deaths. is one among the provinces having highest number of HIV infection.The distribution of HIV infection is mainly in groups with high risk behaviors: infecting drug users 23.5%, prisoners 9.2%, STDs patients 8.4%, commercial sex workers 6.9%, male is four folds female;young age:20-29 makes up 55%. Total of 802 addicted person now in Kien giang, in which 54% are injecting drug users; 20-35% are shared needle and syringe; 20% shared drug bottle, 10% shared cleaning water for needle and syringe. Number of abused drug still increasing especially in Rach gia town. Prostitution activities is multiform and mobile both in the mainland and boat
HIV ; Acquired Immunodeficiency Syndrome ; Sexually Transmitted Diseases ; risk factors

The samples obtained from the 25 healthy adults donors, from19 to 50 years old of age, 18 men and 7 woman. Different antibodies match different methods. Standard method: CD3 (FITC) / CD16, CD56 (PE) / CD45 (PerCP) / CD19 (APC) mixture of antibodies. Estimate method: 3 colors use CD4 (FITC) / CD3 (PE) / CD8 (PerCP) mixture of antibodies and 4 colors use CD3 (FITC) / CD8 (PE) / CD45 (PerCD) / CD4 (APC) mixture of antibodies. Results: standard method - estimate method 3 colors: r = 0,85; % NK (adjust) = 1,99 x % NK (estimated) + 2,31; standard method - estimate method 4 colors: r = 0,85; % NK (adjust) = 1,63 x % NK (estimated) + 4,53
Tissue Donors ; methods ; Reference Standards

The research aimed to establish the maximum time elapsed between collection and analysis of samples. The blood samples were stored at temperature varying from 200C to 280C. Studied on 32 subjects, all samples were marked by the monoclonal antibodies. Each sample had been analyzed 4 times: within 6 hours, 24 hours, 48 hours and 72 hours after drawing. Epi-info 2000 had been used for statistical analysis the results. Results showed that: the maximum elapse of time for the CD4 was 72 hours, for the CD8 was 24 hours and for the lympho and the WBC's as little time as possible, because the results were very different every second time
Blood ; cells ; Temperature

Studying total number of 1,404 HIV infected persons, including 716 AIDS manifestation and 245 AIDS deaths in Dong Thap province from 3/2003 - 4/2003 showed that: the new infectious number of HIV in this province has been increasing quickly; Dong Thap ranks tenth in ten provinces having highest HIV/100,000 people rate (89.53%). Although, the HIV/AIDS epidemic in Dong Thap is still in concentrated period but the distribution of HIV infection with 3% children and 2.8% pregnant women is a worried problem. Moreover, quantity and risk behaviors of HIV/AIDS infection in commercial sex workers and injecting drug users are also worried problems. Basing on these results, Dong Thap should mobilize all potential resources to implement quickly the measures including unnamed test counseling and activities which prevent mother-baby HIV spread helping reduce damaging effect in high risk people
HIV,Acquired Immunodeficiency Syndrome, Sex, Substance-Related Disorders

38 blood samples were divided into 2 groups – normal sample (with 3 groups of cells seen obviously; lymphocyte, mon, gronulocyte on FSC, SSC windows) and abnormal sample group (without these 3 groups of cells).
Antibodies ; cells ; Blood

The objective of study was to establish reference ranged for the lymphocyte subset (TCD3, TCD4, TCD8, B, NK) in peripheral blood of adult donors. This reference is used like target values for patients examinated in HCM city’s Pasteur Institute and then the results were compared with those obtained in Iranian healthy adults. Healthy person’s blood was processed with EDTA anticoagulation then incubated with 2 groups of antibodies from blood donors contained in 2 diverse tubes CD3/CD8/CD45/CD4 and CD3/CD16,56/CD45/CD19. Then through FASC apparatus, the samples were analyzed, and TCD3, TCD4, TCD8, B, NK were determined in each sample diversely.
Tissue Donors ; Adult ; Lymphocyte Subsets

In 6 simples of Nga Truat. 2 simples of Dia Lien, 2 samples of Cu Coc collected in Nghia Trai village. There was no-orgam chlorine pollutants; but in 1 of 9 samples of camomille containing cypermethrine residue exceeded the normal standard
Insecticides ; Medicine, Traditional ; Pharmaceutical Preparations