0.05).No adverse reactions were found during the clinical trial. Conclusion Yinhua Jiedu Granule is effective and safe in treating wind-heat syndrome of upper respiratory infection and influenza.

Objective To establish a method for the determination of tetrahydropalmatine in Fuyantai Capsule. Methods TLC Scanning was used. with n-hexane ∶chloroform ∶methanol(7 ∶3 ∶0.5)as developer,scanned by double-wavelength reflection,?S=275 nm,?R=370 nm,slit 1.2?1.2 mm,SX=3.Results The linear range of tetrahydropalmatine concentration was 0.2～1.0 ?g and r=0.9992. The average recovery was 96.7 %and RSD was 1.92 %.Conclusion This method is accurate with a good reproducibility and can be used for the quality control of Fuyantai capsule.

Objective To establish a method for the determination of naringin in Gongning Granule, Methods The sample was extracted with methyl and the chromatographic conditions were as follows: Kromasil C18 chromatographic column(250 mm?4.6 mm ,5 ?m)with a mobile phase of methyl and 1 %acetic acid (35.5 ∶64.5),the detection wavelength at 283 nm and the flow rate being 1.0mL?min-1. Results A linearity of naringin in Gongning Granule was obtained in the range of 0.0980 ?g to 0.980 ?g,r=0.9997 (n=6).The average recovery was 98.7 %and RSD=1.96 %(n=6). Conclusion This method is easy,sensitive and accurate for the determination of naringin in Gongning Granule.

Objective To determine the content of 5,7-dimethoxycoumarin in Fructus Citris Sarcodactylis. Methods HPLC was used. Mobile phase was methanol-water(65 ∶35),detection wavelength at 326 nm,flow rate 1mL/min and column temperature at 35 ℃.Results The linearity of 5,7-dimethoxycoumarin was in the range of 0.3528～1.7640 ?g. The regression equation was Y=2403.73 X-65.35,r=0.9998. The average recovery was 99.33 %,RSD=1.47 %(n=6). Conclusion The method is simple and accurate. It can be applied in the determination of 5,7-dimethoxycoumarin in Fructus Citris sarcodactylis.

Objective To observe the effects of modified Yigong Powder (MYP) combined with chemotherapy on transplanted hepatocarcinoma of mice and to study its mechanisms. Methods Hepatocarcinoma 22 mouse model was established and then was used to observe the attenuating and synergic effects of MYP when applied together with 5-fluorouracil (5-FU). After 8 days of treatment,the tumor-inhibiting rate,activity of natural killer (NK)cells and interleukin-2 (IL-2) and small intestinal malondialdehyde (MDA) content were examined. Results MYP combined with 5-FU could increased the tumor-inhibiting rate to some extent and improve the immune function by increasing immune organ weight and increasing the activity of NK cells and IL-2. MYP combined with 5-FU could also reduce the 5-FU-induced intestinal injuries by relieving the damage of free radicals and inhibiting the lipid peroxidation and a good prognosis was expected in tumor-bearing animals treated with chemotherapy. Conclusion MYP exerts an attenuating and synergic effect when used together with 5-FU in treating tumor-bearing mice and its mechamism may be related to the improvement of immune function and reduction of intestinal injuries.

Objective To observe the effect of Ganoderma Lucidum (GL) on counteracting lipid peroxidation in cultured vascular smooth muscle cell(VSMC)and to explore its therapeutic mechanism for atherosclerosis. Methods The effects of GL on the content of lipid peroxide(LPO)and the activity of superoxide dismutase(SOD)in medium were studied . Results GL decreased LPO content and increased SOD activity in a dose-effect manner. Conclusion GL can counteract the lipid peroxidation in VSMC and its therapeutic mechanism for atherosclerosis may be related to the counteraction of lipid peroxidation and enhancement of endogenous antioxidase activity.

Objective To study the mechanism of herbal medicines with the actions of tonifying Qi,calming wind and eliminating phlegm in treating epilepsy. Methods Rat models of chronic epilepsy were induced by penicillin. Changes of electrocorticogram (ECOG) and the contents of cerebral gamma-aminobutyric acid (GABA) and glutamate (GLU) in rats were observed. Results Radix Astragali increased the content of GABA and the effect was stronger with Borneol Syntheticum added but had less effect in decreasing GLU. Both Bombyx Batryticatus and Arisaema cum Bile could decrease GLU content and Arisaema cum Bile in particular. The effect of Radix Astragali was stronger in decrease of ECOG frequency but weaker than that in modifying the amplitude of ECOG,while Bombyx Batryticatus and Arisaema cum Bile were in the contrary. The effect of Radix Astragali in improving ECOG did not differ from that with Borneolm Syntheticum added. Conclusion Qi-tonifying,wind-calming and phlegm-eliminating herbal medicines are effective in treating epilepsy and their mechanism may be related to the regulation of electrocorticogram and the content of cerebral GABA and GLU.

Objective To evaluate the effect of Tangshenkang Mixture (TM) on preventing diabetic nephropathy (DN). Methods SD rat model of diabetes was established by intraperitoneal injection of streptozotocin (STZ). Contents of blood sugar,urine sugar and urine albumin in rats were measured. The pathological changes of unilateral kidney were also observed under microscope by HE and PAS staining. Glomerulus dimensions,capillary vessel dimensions and PAS-positive matter area were measured with Pathological Picture Analyzing System. Results ①Obvious albuminuria,enlargement of glomerulus and capillary vessel,increase of PAS-positive matter area were found in model group. ②Urinary protein content in TM group was significantly lower than that in model group and western medicine group(P

Objective To provide evidence for the pharmacological action of blood-activating and blood-stasis-dissipating medicines by studying the influence of Zhenxintong Oral Liquid (ZOL) on thrombus-associated marker levels in coronary heart disease with blood-stasis syndrome. Methods Forty male Wistar rats were randomly allocated to 5 groups: normal group,model group,large-dose ZOL group,small-dose ZOL group and Compound Danshen Drip Pill(CDDP) control group. Blood-stasis models were established by peritoneal injection of dexamethasone (0.25mg?kg-1?d-1). After 8 days of treatment,levels of activated partial thromboplastin time (APTT),antithrombin -Ⅲ(AT-Ⅲ),tissue-type plasminogen activator (t-PA) and tissue-type plasminogen activator inhibitor (PAI) in blood from abdominal aorta were determined. Results AT-Ⅲlevel in the two ZOL groups and in CDDP control group was significantly higher than that in model group(P 0.05). t-PA level was higher and PAI level was lower in large-dose ZOL group than that in CDDP control group (P

Objective To observe the effect of Shengmai Chenggu Prescription (SCP) on the damage of endothelial cells (EC) induced by endotoxin. Methods EC obtained from rabbit's aorta were cultured and were treated with endotoxin and serum containing SCP respectively. Histological changes and function of the cultured cells were observed under light microscope and electron microscope and with MTT method . Results Endotoxin could result in the pathologic injury of cultured EC and inhibit the proliferation of the cells. Serum containing SCP could increase the activity of EC and promote its proliferation. Conclusion SCP can protect endothelial cells from damage induced by endotoxin.