Objective To optimize the preparation process of Tangzu Granula. Methods With the paste-forming rate and total content of paeoniflorin as the indexes, the optimal water-extraction condition was screened by orthogonal design. With hydroscopicity, fluidity and formability as the indexes, the appropriate ratio of the excipient and the formula were selected. Results The optimum water-extraction condition was as follows: 10-fold water, decoction for 2 times and 1.5 hour for each time. The optimal ratio of the excipient and the formula for preparation of Tangzu granules were two proportions of the extract and three proportions of the excipient which consisted of lactose and dextrin(2 : 1, w/ w). Conclusion The technology is effec-tive, scientific, reasonable, and practical.

Objective To study the technique condition for separating and purifying total flavones from Striga asiatica (L.) O. Ktze. by Polyamide. Methods Total flavones content, in the sample solution of Striga asiatica (L.)O. Ktze. , detected by UV spectrophotometry, is used as the index. Some technological parameters are observed by single factor observation. Results Polyamide has good absorbing effect on total flavones of Striga asiatica (L.) O. Ktze. The liquid concentration of its absorbing and separating technological condition is 1.12～2. 24 mg/mL and at the absorbing speed of 2BV/h. The elution effect of 95 % alcohol of 250 mL is the best. Conclusion This method is simple and feasi-ble, fit for separating and purifying total falvones from Striga asiatica (L.) O. Ktze.

Objective To explore the the analytical method for the quality difference of Radix Salviae Mihiorrhizae (RSM) injection. Methods Parallel intercomparison experiment was carried out in different batches of RSM injection samples which had different degrees of adverse drug reaction. The samples were analyzed by gel size exclusion chromatog-zraphy and C18 HPLC fingerprint. Subtractive analysis was used to reveal the information of the non-low molecular weight phenolic acids. Results There were obvious differences between the results of size exclusion chromatography. The amount of the non-low molecular weight phenolic acids from different batches of the samples was also different, which was correlated with the degrees of acute toxicity in guinea pigs. Conclusion The amount of the non-low molecular weight phenolic acids varies with different batches of Radix Salviae Mihiorrhizae injection. It is suggested that non-low molecular weight phenolic acids can be used for the quality control of Radix Salviae Mihiorrhizae injection. For the first time, the perspective that the corresponding relationship between the fingerprint and their toxicities of Traditional Chinese medicine injection may be one of the most important fields is put forward.

Objective To establish a HPLC method for the simultaneous determination of campanulin, paeoniflorin and hydrox-ysafflor yellow A in Buyang Huanwu Decoction. Methods A Kromasil C18(4. 6 mm × 250 mm, 5 μm) column was adopted. The mobile phase consisited of methanol and acetonitrile(26 : 2) (A), and 0. 7 % phosphoric acid solution(B), in gradient e-lution (0～10 min, 25 % →35 % A; 10～25 min, 35 %→40 % A). The flow rate was at 1.0 mL·min-1. The column tem-perature was 35 ℃;, and the detection wavelengths were set at 260nm for campanulin, 230 nm for paeoniflorin and 403 nm for hydroxysamor yellow A. Results The linear ranges of campanuliu, paconiflorin and hydroxysafflor yellow A were 0. 0539～1. 078 μg (r=0. 999 5), O. 4160～8. 320μg (r=0. 999 8), and 0. 0418～0. 8352 μg (r=0. 999 5) respectively; the av-erage recovery was 99. 4 % (RSD=1.5 %), 100. 6 % (RSD=1.7 %), and 101.0 % (RSD=1.9 %) respectively. Conclu-sion The method is simple, feasible and reproducible, and can be used for the quality control of Buyang Huanwu Decoction.

Objective To investigate effects of different solvent, different temperature and different concentration of hydroxypropyl cyclodextrin on solubility of asarone. Method Determination of asarone solubility in different solvent, at different temperature and different concentration of cyclodextrin were carried out by HPLC-UV method. Results The solubility of asarone in water, tea oil, ethanol, and methanol were 0. 1 mg·mL-1 13.44 mg·mL-1, 1.87 mg·mL-1 and 1.76 mg·mL-1, respectively. The concentration and temperature of cyclodextrin showed a positive correlation with solubility of asarone. At normal temperature, the solubility of asarone in water increased 10 times by 45 % hydroxypropyl cyclodextrin. Conclusion Asarone has poor solubility in water and it can be improved by high concentration of hydroxypropyl cyelodetrin.

Objective To establish a method for identification and quality control of Rhizoma Acori Talarinowii. Methods The medicinal herb of Rhizoma Aeori Talarinowii was identified by its origin and TLC. High-performance liquid chromatography (HPLC) was used to determine the content of β-asarone in Rhizoma Acori Talarinowii. Results The results of TLC showed that there were some spots with the same color in the position having the same relative retention time between the tested medici-hal material and the control. The results of HPLC showed that a good iinearity was in the range of 0. 1～0. 6 βg, the regression equation was Y=37158. 2X-589. 5(r=0. 999 7), and the average recovery was 96. 78 % (RSD=0. 95 %). Conclusion The method is simple and reproducible, and can be used for the quality control of Rhizoma Acori Talarinowii.

Objective To establish the quality standard for Huamoyan Tablet. Methods Radix Salviae MiltioiThizae and Radix Cyathulaewere were identified by TLC. The content of protocatechuic aldehyde was determined by HPLC. Results The spots on TLC plates were clear without the interference of negative control. Protocatechuic aldehyde showed a good linear relationship in the range of 0. 021～0. 105 μg, r=0. 999 9. The average recovery was 98. 13 % ,and RSD=2. 41%. Conclusion The method is simple, sensitive and accurate, and can be used to control the qual-ity of Huamoyan Tablet.

Objective To establish a method for quality control of Tangui Weining granules. Methods TLC was used for the identification of Lignum Santali Albi and Cortex Cinnamomi. HPLC was used for the determination of Glycyrrhizin. The chromatographic conditions were as follows: Diamonsil C18(4. 6 mm ×250 mm, 5 μm)was used as a stationary phase, the mobile phase consisted of methanol-O. 2 mol/L ammonium acetate solution-acetic acid (60 : 40 : 1), the ducible, and the negative controll did not show interference. Glycyrrhizin was separated completely by HPLC and showed a good linearity in the range of 0. 1568 to 1. 568μg, r=0. 999 8, the average recovery was 99.69 %, and RSD was 0. 58 %. Conclusion TLC is simple, convenient and accurate. The method of HPLC is proved to be simple and accu-rate with a good reproducibility and can be used for the inspection of Glycyrrhizin in Tangui Weining Granules. This study provides a foundation for quality control of Tangui Weining granules.

Objective To establish a HPLC method for the determination of ferulic acid in Xuefu Zhuyu Decoction. Methods A Kromasil C18(250 mm ×4. 6 mm, 5 μm) column was adopted. The mobile phase consisted of acetonitrile-0. 085 % phos-phoric acid(17 : 83)with the flow rate of 1.0 mL/min. The column temperature was at 35 ℃ and detection wavelength was set at 316 nm. Results A good linearity of ferulic acid was in the range of 0. 0252μg to 0. 504μg and r=0. 999 9. The average recovery was 100. 15 % and RSD=1.69 %. Conclusion The method is simple and rapid, and it is suitable for the determi-nation of ferulic acid in Xuefu Zhuyu Decoction.

Objective To investigate the chemical constituents of Citrus medica var. sareodactylis from Sichuan province, and to provide evidence for the development and utilization and quality evaluation of the medicinal material.Methods Various chromatographic techniques were used to purify the components of this herb. Compounds were identi-fied by their physical characteristics and spectral feature. Results Seven compounds were isolated from Citrus medica var. sarcodactylis, and they were identified as sibiricol (Ⅵ), 7-methylesculetin (Ⅶ), bergapten (Ⅷ), sigmasteryl acetate(Ⅸ), 5-methoxyfurfural(Ⅹ), limonin(Ⅺ), daucosterol (Ⅻ). Conclusion Compound Ⅵ, Ⅶ and Ⅸ are isolated from plants of Rutaceae for the first time.