Margarita Co. Oral Liquid is Prepared from several tonic traditional medicines such as Margarita Rhizoma Polygonati, Fruetus Lycii, Radix Pdygoni Multiflori, Radix Rehmannia Praeparata, etc. A clinical trial on 144 cases showed that this Preparation can tonify liver and kidney, nourish blood and retard aging. It is effective for deficiency of liver and kidney, manifested as dizziness, amnesia and enuresis, debility after long illness, hypertension and hypercholesterolemia. The effective rate is 95.83％ for kidney deficiency, 71.47％ for hypercholesterolemia.

It is considered that formula screening should be in accordance with the heory of Traditional Chinese Medicine, and the composition of formula should be simpliied. The doses of each ingredient and whole formula should be paid attention. In order to arantee the safety ana offectiveness of the preparation, the method of dose screening was roposed.

A gravimetric method for determining the total saponin content in Xuesuantong capsule has been developed. The proposed method has the advantages of efficiency, simplicity and accuracy. The average recovery rate is 97.6％ with a relative standard deviation of 0.78％. AHhough colorimetrg has been employed commonly, there were many factors affecting colour reaction, so thatthe result was not ideal. Now the gravimetric method is applied to control the quality temporarily during the period of clinisal study.

Objective To compare the rutin contents in 7 kinds of commercial Fructus jujubae by HPLC method. Methods The analysis was carried on MN NUCLEODUR C18 column. The mobile phase consisted of acetonitrile-0.05 % H3PO4(15 ∶ 85), flow rate was 1.0 mL/min, determination wavelength was 257 nm and the column temperature was at 40 ℃ . Results The linear range was 0.050 5～ 0.404 4 ? g and r= 0.998 7.The recovery was 96.2 % and RSD 1.6 % (n=6). Conclusion This method can eliminate the interference of sugar ingredients efficiently and is with good repeatability. It is suggested that the plumper the fruits of jujubae, the higher the content of rutin.

Objective: A method for the determination of gallic acid in the extract of Quercus salece- na Blume (Fagaceae) and its preparation (Urocalum Capsules) by means of RP-HPLC was established. Method: The hydrolysable tannins contained in the sample is hydrolyzed with hy- drochloric acid, the hydrolysis component-gallic acid is determined by HPLC using RP-C18 column, 0.005mol. L-1 of phosphoric acid-methanol (98: 2) as mobile phase , 270 nm as detec- tion wavelength. Result: The linear range of gallic acid is 0. 3～3. 1?g , r=0. 9999, the average recovery is 100. 4%. Conclusion: It is confirmed that the established method for assay of gallic acid meet the criteria of quantitative analysis of herbal medicine by validation of hydrolysis condition, repeatebility, stability and recovery, The method is simple , good reproducible and precise , and it can also be applied to analysis of other herbal medicine containing hydrolysable tannins.

Objective: To review the influence of various processing methods on the content of baicalin in radix Scutellariae and its preparations. Method: The content of baicalin was determined by HPLC. Results: The content of baicalin decreased evidently when the brocessing method was in appropriate. Conclusion: Improper processing method can decrease the content of baicalin, and frying and carbonizing destroyed baicalin seriously.

Proteins from dried roots of three medicinal Panax species (P. Quinquefolius, P. ginseng and P. nologinseng) were analyzed by SDS-PAGE and Western-blot. The protein fin- gerprints of Panax species were confirmed. From 28KD to 58KD and 55KD are in common. Very specific fingerprints of P. quinquefolius are found below 28KD. The fingerprints above 58KD seemed to be peculiar to P. ginsing. Hence the Western-blot approach can be used as a means to authenticate the concerned Panax species, especially for identification of P. quinquefolius.

The effects of Xiangsha Liujunzi Decoction (XLD) on expermental bile-regurgitational gastritis and gastric evacuation in rats were observed. XLD in dose of 30g/kg (p.o.) markedly pre- vented the acute gastric ucosa damate induced by gastric feeding of alkaline intestinal contents. For chronic regurgitational gastritis rat model induced by pyloric dilation with regurgitation of duodenal juice after 2 and 4 weeks , XLD in dose of 30g/kg (p. o.) lowered the incidence of gastritis, and reduce intragastric pH and bile acid. XLD in dose of 15g/kg and 30g/kg (p.o.) markedly inhibited the gastric evacuation. The results suggested that XLD is available for the treament of bile-regurgi- tational gastritis.

Pleuritis rat model was adopted to observe the influence of Eryngium foetidun L . on in- flammatory cell factors (TNF-a, IL- 6 and NO) . The results showed that Eryngium foetidum L. can decrease the contents of TNF-a , IL- 6 and NO in serum. It is indicated that the anti- inflam- matory effect of Eryngium foetidum L. is probably related to the decrease of the contents of the above factors.

Objective: Effects of Shenmai injection and aminophylline alone or in combination on transdiaphragmatic pressure and electrical activity of diaphragm were observed, so as to evaluate their effecfs on diaphragmatic fatigue. Methods: The diaphramatic fatique (DiF) model was estab- lished by stimulating bilateral phrenic nerves for 40 minutes. The animals were allocated to three groups: Shenmai group (2mL/kg, N=7), aminophylline group (20mg/kg, N=7) and combi- nation group (Shenmai injection 2mL/kg+aminophylline 20mg/kg, N=7) The transdi- aphragmatic pressure (Pdi) and diaphragmatic electromyogram (EMGdi) were recorded before and after treatment. EMGdi was analysed by computer and then the high/low frequency ratio (H/L) and the central frequency (Fc) were caculated. The diaphragm evoked potential (DEP) was record- ed simutaneously. All data was analysed by analysis of variance and q test. Results: Pdi, H/L, Fc and amplitude of DEP were markedly increased after the treatment with aminophyline and Shen- mai injection alone (Compared with DiF. P