1.Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae.
Yan JIN ; Chenghua SHEN ; Sun HUH ; Woon Mok SOHN ; Min Ho CHOI ; Sung Tae HONG
The Korean Journal of Parasitology 2013;51(4):433-439
Toxocariasis is a worldwide zoonosis caused by larvae of ascarid nematodes of dogs or cats, Toxocara canis or T. cati. Diagnosis of human toxocariasis currently relies on serology that uses T. canis excretory-secretory antigen to detect specific IgG antibodies by ELISA. We investigated the serodiagnostic efficacy of ELISA using crude antigen of T. canis larvae (TCLA). Serum specimens of 64 clinically confirmed toxocariasis, 115 healthy controls, and 119 other tissue-invading helminthiases were screened by ELISA using TCLA. The ELISA using TCLA showed 92.2% (59/64 patient samples) sensitivity and 86.6% (103/119) specificity. Its positive diagnostic predictivity was 78.7% and negative predictivity was 97.8%. No serum of healthy controls reacted but that of anisakiasis (45.5%), gnathostomiasis (19.2%), clonorchiasis (15.8%), sparganosis (11.1%), and cysticercosis (6.3%) cross-reacted. Immunoblot analysis on TCLA recognized antigenic proteins of 28- and 30-kDa bands in their dominant protein quantity and strong blotting reactivity. The present results indicate that the ELISA using our TCLA antigen is acceptable by the sensitivity and specificity for serodiagnosis of human toxocariasis. ELISA with TCLA is recommended to make differential diagnosis for patients with any sign of organ infiltration and eosinophilia.
Adolescent
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Adult
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Aged
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Animals
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Antigens, Helminth/chemistry/*diagnostic use/immunology/isolation & purification
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Cats
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Dogs
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Enzyme-Linked Immunosorbent Assay/*methods
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Female
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Humans
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Larva/chemistry/immunology
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Male
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Middle Aged
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Serologic Tests
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Toxocara canis/chemistry/*immunology
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Toxocariasis/*diagnosis/immunology/parasitology
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Young Adult
2.Molecular Diagnosis of an Ocular Toxocariasis Patient in Vietnam.
Nguyen VAN DE ; Nguyen Vu TRUNG ; Le Van DUYET ; Jong Yil CHAI
The Korean Journal of Parasitology 2013;51(5):563-567
An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocara was collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the eye by a molecular method was performed for the first time in Vietnam.
Adult
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Animals
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Base Sequence
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DNA, Helminth/chemistry/genetics
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DNA, Ribosomal/chemistry/genetics
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DNA, Ribosomal Spacer/chemistry/genetics
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Eye Infections, Parasitic/*diagnosis/parasitology
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Humans
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Larva
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Male
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Molecular Sequence Data
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Phylogeny
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Sequence Alignment
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Sequence Analysis, DNA
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Toxocara canis/classification/genetics/*isolation & purification
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Toxocariasis/*diagnosis/parasitology
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Vietnam
3.Effects of Toxoplasma gondii and Toxocara canis Antigens on WEHI-164 Fibrosarcoma Growth in a Mouse Model.
Hossein Yousofi DARANI ; Hedayatollah SHIRZAD ; Fataneh MANSOORI ; Nozhat ZABARDAST ; Mahdi MAHMOODZADEH
The Korean Journal of Parasitology 2009;47(2):175-177
Cancer is the main cause of death in developed countries. However, in underdeveloped countries infections and parasitic diseases are the main causes of death. There are raising scientific evidences indicating that parasitic infections induce antitumor activity against certain types of cancers. In this study, the effects of Toxoplasma gondii and Toxocara canis egg antigens in comparison with Bacillus Calmette Guerin (BCG) (known to have anticancer distinctive) on WEHI-164 fibosarcoma transplanted to BALB/c mice was investigated. Groups of 6 male BALB/c mice injected with T. gondii antigen, BCG, or T. canis egg antigen as case groups and alum alone as control groups. All mice were then challenged with WEHI-164 fibrosarcoma cells. The mice were examined for growth of the solid tumor and the tumor sizes were measured every other day up to 4 wk. The mean tumor area in T. gondii, BCG, or alum alone injected mice in 4 different days of measurements was 25 mm2, 23 mm2, and 186 mm2 respectively. Also the mean tumor area in T. canis injected mice in 4 different days was 25.5 mm2 compared to the control group (alum treated) which was 155 mm2. T. gondii parasites and T. canis egg antigens induced inhibition of the tumor growth in the fibrosarcoma mouse model. We need further study to clarify the mechanisms of anti-cancer effects.
Animals
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Antigens, Helminth/isolation & purification/pharmacology/*therapeutic use
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Antigens, Protozoan/isolation & purification/pharmacology/*therapeutic use
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Antineoplastic Agents/isolation & purification/pharmacology/*therapeutic use
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Chemoprevention/*methods
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Female
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Fibrosarcoma/pathology/*prevention & control
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Male
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Mice
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Mice, Inbred BALB C
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Mycobacterium bovis
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Toxocara canis/*chemistry
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Toxoplasma/*chemistry