2.Immunogenic evaluation and test strategy for medical devices.
Chenghu LIU ; Ping WU ; Yanping SHI ; Min WANG
Chinese Journal of Medical Instrumentation 2012;36(1):56-58
This paper emphasizes on the strategy for immunogenic evaluation of medical devices. expecting to fulfill the scientific procedure for immunogenic evaluation of medical devices and to improve the standard systems for biological evaluation of medical devices in the future.
Equipment Safety
;
methods
;
Toxicology
;
methods
3.Development and application of adverse outcome pathway in toxicology research.
Yanhua WANG ; Huawei DUAN ; Email: HUAWEIDUAN@126.COM.
Chinese Journal of Preventive Medicine 2015;49(12):1115-1118
Adverse outcome pathway (AOP) was a conceptual construct that integrated existing knowledge concerning the pathway of causal linkages between a molecular initiating event (MIE) and a final adverse effect at individual or population levels. The AOP methodology could be used as a basis for effects extrapolation and was an approach towards providing a framework for collecting and evaluating relevant chemical, biological and toxicological information. The framework would play an important role in risk assessment. We reviewed the concept of AOP, the development and assessment of the framework and the established models in toxicology researches. And the prospects and challenges of its application in toxicology were also introduced.
Research Design
;
Risk Assessment
;
Toxicology
;
methods
4.Research Progress on Biological Matrix Reference Materials in Forensic Toxicology.
Hang CHEN ; Jing HU ; Zheng QIAO ; Hong-Xiao DENG ; Min LÜ ; Wei LIU
Journal of Forensic Medicine 2023;39(2):176-185
Biological matrix reference material is a reference material that combines the target material with the biological matrix. The biological matrix reference material has higher consistency with the authentic specimens in forensic toxicology, and its application has a positive effect on improving the accuracy of test results. This paper reviews the research on the matrix reference materials corresponding to three common biological test materials (blood, urine and hair). In order to provide reference for the development and application of biological matrix reference materials in forensic toxicology, this paper mainly introduces the research progress of preparation technology of biological matrix reference materials and some existing products and their parameters evaluation.
Forensic Toxicology/methods*
;
Hair
;
Body Fluids
5.Development and Application of Metabonomics in Forensic Toxicology.
Journal of Forensic Medicine 2015;31(3):219-226
Metabonomics is an important branch of system biology following the development of genomics, transcriptomics and proteomics. It can perform high-throughput detection and data processing with multiple parameters, potentially enabling the identification and quantification of all small metabolites in a biological system. It can be used to provide comprehensive information on the toxicity effects, toxicological mechanisms and biomarkers, sensitively finding the unusual metabolic changes caused by poison. This article mainly reviews application of metabonomics in toxicological studies of abused drugs, pesticides, poisonous plants and poisonous animals, and also illustrates the new direction of forensic toxicology research.
Animals
;
Biomarkers
;
Forensic Toxicology/methods*
;
Humans
;
Metabolomics/methods*
6.Detection and application of ethyl glucuronide in forensic toxicology.
Hui ZHAO ; Xian-yi ZHUO ; Bao-hua SHEN
Journal of Forensic Medicine 2009;25(1):63-67
Ethyl glucuronide is a specific metabolite of ethanol. There have been plenty of articles referring its pharmacokinetics, detection and application as a specific bio-marker of alcohol intake. This article reviews various analytical methods of EtG, relationship between EtG quantification and ethanol intake, and criteria for determining chronic alcohol abuse, and origin of ethanol found in the cadavers by EtG analysis. EtG has its potential application in forensic toxicology.
Alcoholism/metabolism*
;
Forensic Toxicology/methods*
;
Glucuronates/urine*
;
Hair/chemistry*
;
Humans
7.Measurement of acetonitrile in blood and urine by head-space gas chromatography.
Ru-Xin LUO ; Xian-Yi ZHUO ; Bao-Hua SHEN
Journal of Forensic Medicine 2012;28(6):422-425
OBJECTIVE:
To establish the method for measurement of acetonitrile in blood and urine by head-space gas chromatography.
METHODS:
DB-ALC1 (30 m x 320 microm x 1.8 microm) and DB-ALC2 (30 m x 320 microm x 1.2 microm) capillary column were used to measure the acetonitrile in blood and urine with the isopropanol as internal standard reference.
RESULTS:
The limits of detection of acetonitrile in both blood and urine were 0.5 microg/mL, with a linear range of 5-1000 microg/mL (r = 0.999).The accuracy of this method was 93.2%-98.0%. The RSD for the intra-day and inter-day were less than 3.7%.
CONCLUSION
The method is capable for measurement analysis of acetonitrile in blood and urine.
Acetonitriles/urine*
;
Chromatography, Gas/methods*
;
Cyanides/urine*
;
Forensic Toxicology/methods*
;
Humans
;
Reproducibility of Results
;
Suicide, Attempted
8.Cytomics: a new chance and promise for pharmacology and toxicology research.
Journal of Zhejiang University. Medical sciences 2007;36(3):209-216
Animals
;
Cytological Techniques
;
methods
;
Cytophotometry
;
instrumentation
;
methods
;
Humans
;
Pharmacology
;
methods
;
trends
;
Systems Biology
;
methods
;
Toxicology
;
methods
;
trends
10.How to validate a bio-analytical method.
Ping XIANG ; Min SHEN ; Xian-Yi ZHUO
Journal of Forensic Medicine 2008;24(1):60-65
It is imperative that any newly established bio-analytical method is validated thoroughly, using standardised parameters. The purpose of this article is to provide recommendations on how to validate a new bio-analytical method. Based on author's personal experience and some interesting discussion points from the conference of "International Association of Forensic Toxicologists" in 2007, the authors propose these essential requirements for validating a new analytical method. The key parameters of method validation include selectivity, linearity, accuracy, precision, LOD (limit of detection), LLOQ (the lower limit of quantitation), stability and the extraction recovery. For any bio-analytical method using LC-MSn (Liquid chromatography-mass spectrometry), studies of matrix effect should also be included in addition of the above parameters.
Chemistry Techniques, Analytical/standards*
;
Chromatography, Liquid/methods*
;
Forensic Toxicology
;
Mass Spectrometry/methods*
;
Reproducibility of Results
;
Validation Studies as Topic