In this study, we aimed to clarify awareness regarding genetic testing among pharmacists in Hiroshima City to contribute to regional medical care provided in pharmacies. Pharmacists should consider the advantages and disadvantages of being able to easily receive genetic tests. We conducted an awareness survey on genetic testing among pharmacists belonging to the Hiroshima Pharmaceutical Association, at 645 pharmacies in Hiroshima City. Responses were received from 674 pharmacists, yielding a response rate of 43.4% (280 pharmacies). In response to the question, “Can you perform genetic testing at a pharmacy?”, 6.7% of pharmacists answered yes and 65.4% said this was impossible. Among the 92.4% of pharmacists other than those available, 79.6% answered “I am worried about my knowledge of genetic testing,” followed by 39.3% “cannot take the time,” 29.2% “hurdles for new business,” 28.1% “problems such as pharmacy facilities to protect personal information,” and 15.7% “ethical difficulties.” From the age difference, pharmacists do not carry out genetic testing even if they have knowledge; they need to make an ethical judgment to determine whether to conduct genetic testing. Additionally, although pharmacies provide medication guidance and protect personal information, 28.1% of pharmacists answered that there are problems with their pharmacy’s ability to protect personal information when handling “genetic information.” Pharmacists should consider it critical to treat “genetic information” as personal information, with the utmost care and understanding.