ObjectiveTo investigate whether the reactive oxygen species involved in inhibiting proliferation of human bone marrow cells of neuroblastoma cells (SH-SY5Y)process by giant typhonium rhizome water extract,and to investigate the initial mechanism of anti-tumor effect of giant typhonium rhizome in vitro.MethodsAfter giant typhonium rhizome water extract processing of human bone marrow neuroblastoma cells (SH-SY5Y)at 24 h and 48 h,the cells were collected,and western blotting reaction methods such as flow cytometry was used to study the levels of ROS and SH-SY5Y apoptosis.ResultsThe levels of ROS elevated from before dosing (4.9±1.6)％ to (39.7 ± 11.8)％ in the process of giant typhonium rhizome water extract induced apoptosis in SH-SY5Y; after ROS inhibition was added,SH-SY5Y apoptosis level induced by giant typhonium rhizome water extract had decreased.ConclusionROS involved in the process of giant typhonium rhizome water extract inhibiting cell proliferation in SH-SY5Y.

ObjectiveTo investigate the anti tumor effect of Giant Typhonium Rhizome.Methods SH-SY5Y was treated for 24 or 48 h by Giant Typhonium Rhizome.Cell Counting Kit-8 (CCK-8) cell proliferation and cytotoxicity assays were applied to detect the inhibition of SH-SY5Y cells treated with aqueous extract of white monkshood root.AV/PI double staining flow cytometry was applied to explore the mechanism of growth inhibition.ResultsSH-SY5Y cells could be inhibited by root aqueous extract of Giant Typhonium Rhizome,the IC50 at 48 h (50％ inhibitory concentration) was 0.862 mg/ml,while the IC50 at 24 h was 1.158 mg/ml.Western-blot results showed that root aqueous extract of Giant Typhonium Rhizome could induce apoptosis to inhibit tumor cells.ConclusionThe Rhizoma typhonii inhibit the growth of SH-SY5Y.

Objective To investigate the association between serum total testosterone (TT ) levels and type 2 diabetes(T2DM )and any gender differences in elderly patients. Methods Based on the Aging Health database built from 2008 to 2012 ,935 elderly individuals over 60 years old with a mean age of 65.8 years receiving physical examinations were included.According to the 1999 WHO criteria for diabetes ,participants were assigned into four groups :a T2DM group(n＝298) ,an impaired fasting glucose(IFG)group(n＝26) ,an impaired glucose tolerance(IGT)group(n＝121) ,and a normal glucose regulation(NGR)group(n ＝ 490).We measured serum TT by ELISA and analyzed the distribution patterns in the groups.Furthermore ,we examined the gender-specific correlation of TT with T2DM and homeostasis model assessment of insulin resistance (HOMA-IR). Results The prevalence of T2DM in the participants was 31.9%(298/935).One-way ANOVA analysis showed that the TT level was higher in the NGR group than in the T2DM and IGT groups (PANOVA＝ 0.001) . Logistic regression analysis indicated a significant protective association between TT and T 2DM in the elderly.Every one unit of increase in the SD of the TT level was accompanied by a 23% reduction in the risk for T2DM (P＝ 0.001).Further gender-stratification analysis suggested that the protective role of TT against T2DM only existed in males (OR＝ 0.55 ,95% CI :0.44-0.68 ;P＜ 0.001).After adjustment for age ,blood pressure ,blood lipids ,and waist circumference ,the protective role of TT against T2DM in males still remained (OR ＝ 0.68 ,95% CI :0.53-0.86 ;P ＝ 0.002 ).Pearson correlation analysis also indicated a significant negative correlation between TT and HOMA-IR in older males(P＝0.002).As for older females ,no significant correlation of TT with T2DM and HOMA-IR was found. Conclusions The serum TT level might be an independent protective factor for T 2DM in older males ,as evidenced by its correlation with improved insulin resistance status ,which is not present in older females.