with hyperbilirubinemia admitted to the Neonatology Department of the First Affiliated Hospital of Zhengzhou University from January 2016 to October 2018 were divided into the control group (n=51) and the observation group (n=51) by random number table method. Both groups underwent auditory brainstem response hearing screening. The control group was given routine nursing intervention, the observation group was given non-drug sedation nursing intervention. The sedative effects of the two groups were compared. The Score of Newborn Facial Coding System (NFCS) and effective rates of examination between the two groups were compared. Results? After intervention, mean heart rate and mean respiratory rate in the observation group were statistically lower than those in the control group (P＜0.05), and average sleep latency was significantly shorter than that in the control group with statistical difference(P＜0.05). After intervention, only NFCS score in the control group significantly increased compared with that before intervention, and the NFCS score of the observation group was significantly lower than that of the control group during and after screening with statistical difference (P＜0.05). The effective rate of examination in the observation group was 98.04%, significantly higher than that of the control group (86.27%) with statistical difference(χ2=4.883,P＜0.05). Conclusions? The application of non-drug sedative nursing intervention in auditory brainstem response in hyperbilirubinemia neonatal hearing screening has good sedative effect, can significantly reduce the stress response of children to the outside world, and improve the efficiency of examination. It has certain application value in neonatal medical examination.