Objective:To understand the current status of health-related hardiness among breast cancer patients undergoing radiotherapy and to analyze its influencing factors, and to provide evidence for the development of psychosocial care programs for breast cancer patients undergoing radiotherapy.Methods:A total of 126 postoperative breast cancer patients undergoing radiotherapy were selected from the First Affiliated Hospital of Dalian Medical University from January to September 2018. The self-designed general data questionnaire and Health-related Hardiness Scale (HRHS) were used to analyze the current status and the influencing factors of health-related hardiness among patients.Results:The overall score of health-related hardiness among breast cancer patients undergoing radiotherapy was 127.61±20.19, which was located at the low medium level. There were significant differences in the scores of patients of different ages, education background, marital status, fertility status, children status, family history, religion, family income, and medical insurance forms ( t value was -3.800-8.128, F value was 8.906-274.271 P<0.01). The regression analysis showed that the influencing factors of health-related hardiness were age, family income, marital status, education background, family history, and religion. The degree of the dependent variable was 89.4%. Conclusions:Breast cancer patients undergoing radiotherapy have a low medium level of health-related hardiness, and are affected by age, family income, marital status, education background, family history, and religion.

Objective To found sensitive and reliable method to identify trophoblastic tumor cells in the peripheral blood of the patients suffered from gestational trophoblastic tumor.Methods Given numbers of JAR cell from ten to million were mixed into 10ml non pregnant peripheral blood as a model. Detection of ? hCG mRNA with fluorescence quantitative reverse transcription polymerase chain reaction (FQ RT PCR) and then estimation of the numbers of tumor cell in the blood. Nine cases of peripheral blood were collected from the pretreatment patients of gestational trophoblastic tumor to assay ? hCG mRNA with FQ RT PCR, then to estimate the numbers of tumor cell in the circulation blood. Results FQ RT PCR could detect ? hCG mRNA when ≥10 2 JAR cells were mixed into 10ml non pregnant peripheral blood. Four cases of bloods had been detected ? hCG mRNA expression in 9 cases of gestational trophoblastic tumor, and the numbers of tumor cell from 10 4 to 10 7 per 10ml blood. Conclusion FQ RT PCR of which primers and probe are designed for ? hCG had been proved to be very sensitive detection means, it could be used to detect gestational trophoblastic tumor cells from patient preipheral blood; With FQ RT PCR the tumor cells had been detected in some patients of gestational trophoblastic tumor.

Objective: To explore the effect of resveratrol (Res) on angiogenesis of human umbilical vein endothelial cells (HUVEC) with the possible mechanisms in vitro.
Methods: The HUVECs were cultured in 6 groups.①Control group, HUVEC were cultured with high glucose in DMEM,②Res group, the cell were cultured with Res at different concentrations,③Res with PI3K blocker LY294002 (Res+Blocker 1) group, ④Blocker 1 group, HUVEC were cultured with LY294002 alone, ⑤Res with eNOS blocker L-NAME (Res +Blocker 2) group and ⑥Blocker 2 group. The effect of Res on HUVEC proliferation was detected by CCK-8 kit, the protein expressions of p-Akt, p-eNOS were examined by Westin blot analysis, nitric oxide (NO) level was measured by nitrate reduction method and the endothelial cell migration was assayed by transwell chamber method.
Results: ① Compared with Control group, HUVEC proliferation increased in Res (1, 5μmol/L ) group, P<0.01, the proliferation in Res (5μmol/L) group was higher than those in Res (0.2, 10, 20μmol/L) group, P<0.01, while Res (20 μmol/L) group could inhibit the proliferation P<0.01. ②Compared with Control group, Res (5μmol/L) group had the higher protein expressions of p-Akt, p-eNOS, P<0.05-0.01, higher NO level, P<0.05.③Compared with Res group, Res+Blocker 1 group had lower expressions of p-Akt, p-eNOS, P<0.01, lower NO, P<0.05; the expressions of p-Akt, p-eNOS and NO level were similar between Res+Blocker 1 group and Blocker 1 group, all P>0.05.④Compared with Control group, the cell migration and tubing formation were higher in Res (5μmol/L) group, P<0.01;compared with Res group, the cell migration and tubing formation were lower in Res+Block2 group, P<0.01.
Conclusion: Res could up-regulate NO level via activating PI3-K/Akt/eNOS signaling and therefore, improving the proliferation, migration and tubing formation of HUVEC in vitro.

Objective To investigate the clinical significance of CD14+HLA-G+ monocytes in pe-ripheral blood and the soluble form of HLA-G ( sHLA-G ) in plasma among patients with gastric cancer ( GC) . Methods Blood samples were collected from 135 patients with gastric cancer ( GC group) , 150 pa-tients with chronic gastritis ( CG group) and 80 healthy controls ( HC group) . Flow cytometry analysis and ELISA were used to detect the percentages of CD14+HLA-G+ monocytes in peripheral blood samples, the concentrations of sHLA-G in plasma samples and the levels of alpha fetoprotein (AFP), cacino-embryonic antigen ( CEA) , CA19-9 and CA125 in serum samples. Mann-Whitney U test was performed to analyze the differences between different groups. The feasibility of using CD14+HLA-G+ monocytes, sHLA-G, AFP, CEA, CA19-9 and CA125 as potential biomarkers to differentiate patients with GC from those with CG or healthy subjects was assessed by using receiver operating characteristic ( ROC ) curve analysis. Results The median percentages of CD14+HLA-G+ monocytes in subjects from GC, CG and HC groups were 18. 6% (12. 1%-26. 7%), 7. 3% (4. 2%-11. 0%) and 4. 6% (3. 6%-6. 3%), respectively. The percentages of CD14+HLA-G+monocytes in the peripheral blood of patients with GC were significantly higher than those in patients with CG and healthy subjects (P<0. 001). The concentrations of sHLA-G in plasma samples collected from patients with GC [(100. 6±61. 3) U/ml) were significantly higher than those in pa-tients with CG [(59.5±19. 9) U/ml) and healthy subjects [(45. 8±23. 3) U/ml] (P<0. 001). ROC curve analysis showed that in terms of GC diagnosis, the area under ROC curve ( AUC) , cutoff value, sensi-tivity and specificity for CD14+HLA-G+monocytes and sHLA-G in plasma were 0. 893 and 0. 720, 12% and 85 U/mL, 75. 8% and 50. 5%, 86. 7% and 95. 9% (P<0. 001), respectively, which indicated that CD14+HLA-G+ monocytes and sHLA-G were better than AFP, CEA, CA19-9 and CA125 in differentiating GC from CG and HC. Moreover, the multivariate logistic regression analysis revealed that the CD14+HLA-G+ monocytes, sHLA-G in plasma as well as CA19-9 and CA125 in serum were positively correlated with the risk of GC after excluding the differences caused by age and gender factors. Conclusion The levels of CD14+HLA-G+ monocytes in peripheral blood and sHLA-G in plasma increased dramatically in patients with gastric cancer, which suggested that CD14+HLA-G+monocytes and sHLA-G might be risk factors for GC and could be used as potential biomarkers for the diagnosis of GC.

Objective To observe the level of supportive care need and quality of life in patients postoperation for breast cancer and ac-cepting chemotherapy, and the relationship between them. Methods From September, 2015 to June, 2016, 235 patients with breast cancer af-ter surgery were conveniently sampled, and investigated with the general situation questionnaire, Supportive Care Need Survey Short-form (SCNS-SF34) and Functional Assessment of Cancer Therapy-Breast (FACT-B). Results A total of 230 patients were analyzed. Patients self-reported to need supportive care in all dimensions of SCNS-SF34, and the most frequency was in the health information (31.30％). The score of FACT-B was (93.25±23.53), that needed further improvement. The needs of supportive care in each dimension negatively correlat-ed with the scores of FACT-B in the dimensions (|r|>0.168, P<0.05) except society/family and function. Conclusion The patients with breast cancer need variety of supportive care, which may impact their quality of life.

Objective: To explore the feasibility of arterial spin labeling (ASL) imaging in assessment of cerebral blood flow (CBF) in patients with internal carotid artery stenosis. Methods: Plain MR, DWI, three dimensional time of flight (3D-TOF) and ASL scanning were performed in 30 patients with unilateral internal carotid artery stenosis. Then the patients were divided into mild stenosis, moderate stenosis and severe stenosis cases according to 3D-TOF imaging. CBF of basal ganglia, centrum semiovale, anterior watershed, frontal lobe and temporal lobe were calculated and compared between the healthy side and the affected side. Results: One side internal carotid artery stenosis was mild in 11 cases, moderate in 9 cases and severe in 10 cases. There was no significant difference of CBF between the healthy side and the affected side of basal ganglia, centrum semiovale, anterior watershed, frontal lobe nor temporal lobe in patients with mild and moderate stenosis (all P> 0.05), while CBF of healthy side of each brain area in patients with severe stenosis were all higher than in the affected side (all P< 0.05). CBF value decreased with the increase of stenosis degree and negatively correlated with the latter (r=-0.966, P< 0.001). Conclusion: ASL can quantitatively and accurately evaluate CBF in patients with internal carotid artery stenosis, which is of great significance for guiding clinical treatment and evaluating curative effect.

Breast cancer is one of the most common cancer among females.With the rise of the concept of positive psychology,the research of positive psychology of patients with breast cancer has become a current hot topic.This research reviews the development of this part from aspects of ″the influence of positive psychological factors on the quality of life in patients with breast cancer″and ″the research status of the positive psychological intervention of patients with breast cancer″,in order to provide the reference for building a better psychological nursing intervention model of patients with breast cancer.

With the development of human centered medical nursing model, empowerment as a new and effective nursing intervention model has been widely concerned. In this paper, from the overview of empowerment theory, the influencing factors of the implementation of empowerment theory in patients with chronic heart failure and the strategies to improve the implementation effect of empowerment theory for patients with chronic heart failure. The purpose of this review is to provide reference for the implementation of empowerment theory for patients with chronic heart failure in China.

PhaseⅡexercise rehabilitation of patients with myocardial infarction is the core stage of the whole exercise rehabilitation process, which plays a vital role in disease rehabilitation and prevention. Previous reviews are difficult to highlight the changes and focus of phaseⅡexercise rehabilitation in patients with myocardial infarction. This article provides a general introduction from the perspective of myocardial infarction patients′ phaseⅡexercise rehabilitation. It summarizes the current status and deficiencies of exercise load assessment method, the types of exercise and effect of the myocardial infarction patient′s phaseⅡexercise rehabilitation, in order to provide reference for the improvement of related studies on exercise rehabilitation.

In order to meet the needs of national development and people′s health, the development of nursing education is facing unprecedented challenges. Nursing practice teaching undertakes the important task of providing talents for the national health cause, and it is urgent to explore a teaching mode that can adapt to the development of the times. In order to provide reference for the further development of clinical nursing education in China, we can establish the clinical nursing practice teaching mode with professional competence as the core from the aspects of clarifying the current situation, innovating the teaching model and improving the ideological and political education quality.