Objective To investigate the feasibility and significance of laparoscopic choledocholithotomy with T-tube drainage. Methods A total of 60 cases of common bile duct stones complicated with gallbladder stones were treated with laparoscopic cholecystectomy and common bile duct exploration with T-tube drainage. Results The operation was performed successfully in all the cases,without conversions to open surgery.Bile leakage occurred in 2 cases and was cured with conservative treatment.Residual stones were found in 6 cases and a postoperative choledochoscopy was required.The operative time was 90~180 min(mean,110 min),the blood loss was 10~50 ml(mean,20 ml).Follow-up checkups in 53 cases(88.3%) for 2~33 months(mean,13.2 months) revealed no abdominal pain,fever,or jaundice.Conclusions Laparoscopic cholecystectomy and common bile duct exploration for the treatment of common bile duct stones complicated with gallbladder stones is feasible and minimally invasive.

OBJECTIVE:To study the anti-inflammatory action of Xiaoyan Quzhi tablets(XQT).METHODS:To observe the degree of swelling of rat feet and the changes of dry-weight and wet-weight of cotton-ball granulation tissue after using the XQT for one week.RESULTS:The XQT could obviously inhibit the swelling of the feet with significant differences between experiment group and control group(P

OBJECTIVE:To study the effects of Xiaoyan Quzhi tablet(XYQZ) on specific and non-specific immunologic functions in mice METHODS:The phagocytosis of mononuclear phagocytes in mice was measured by carbon powder clearance method Chicken serum hemolysin level in the mice immunized by chicken red blood cell was measured with 721-spectrophotometer RESULTS:XYQZ could obviously increase the phagocytosis of mononuclear phagocytes and promote B-lymphocytes to produce serum antibody There were significant differences between experiment group and control group(P

BACKGROUND:Combination of biological and optical technique is used to study the molecular mechanism of cell proliferation and apoptosis, which has become the study hotspot in the filed of bioengineering.OBJECTIVE: The goal of this study was to study the molecular mechanism of XAP-induced apoptosis in single living human lung adenocarcinoma (ASTC-a-1) cells by using confocal fluorescence microscopy imaging and fluorescence resonance energy transfer (FRET) techniques.DESIGN: A controlled observation.SETTING: Institute of Laser Life Science (Key Laboratory of Education Department), South China Normal University.MARERIALS: This experiment was carried out in the Institute of Laser Life Science (Key Laboratory of Education Ministry), South China Normal University between October 2006 and March 2007. Human lung adenocarcinoma (ASTC-a-1) cells were cultured in our laboratory. Xiaoaiping (XAP) parenteral solution was purchased from Tonghua Shenyuan Pharmaceutical Co.,Ltd (No. Z20025869), and G418 was purchased from Huamei Biological Company. SCAT3 was provided by Professor Masayuki Miura. Auto microplate reader (infinite M200, Tecan,Austria)and mitochondrial location fluorescent probe (Mitertracker Red)was purchased from Molecular Probe Company.METHODS: ① The inhibition of XAP at different doses to ASTC-a-1 cell viability was detected by CCK-8. ② Dynamical change of caspase 3-induced by XAP was detected by confocal fluorescence microscopy imaging and fluorescence resonance energy transfer (FRET) techniques. ③The fluorescence emission spectrum of SCAT3 was detected by confocal fluorescence microscopy imaging at different time points after XAP treatment.MAIN OUTCOME MEASURES: ①Cell viability after XAP treatment. ② Dynamic change of fluorescence resonance energy transfer efficiency of SCAT3 detected after XAP treatment. ③ Dynamical change of mitochondrial morphology after XAP treatment.RESULTS: ①XAP inhibited the viability of tumor cells dose-dependently. ② XAP induced the activation of intracellular caspase 3. ③Some mitochondria became dot, and some were cracked after XAP treatment.CONCLUSION: XAP can induce the apoptosis of ASTC-a-1, and caspase 3 is involved in the regulation course.

0.05).After 3 weeks the model rats body weight degraded obviously(P

Objective To investigate the preventive and therapeutic effects of Shenlixin Granules on chronic renal failure rats.Methods The chronic renal failure rat models were caused by adenine.After the treatment of Shenlixin Granules,the body weight,urine volume and renal index of models were examined.Results Shenlixin Granules could obviously inhibit the decrease of body weight and urine volume,reduce the renal index and had obvious protective effect on the renal tissue.Conclusions Shenlixin Granules has a preventive and therapeutic effect on chronic renal failure.

Objective To investigate the clinical characteristics of pulmonary hypertension(PH) in patients with chronic obstructive pulmonary disease (COPD) and study the related risk factors.Methods Patients with stable COPD enrolled in this study,undergoing examinations including full pulmonary function tests (PFT),6-minute walk distance (6MWD),Exercise Oxyhemoglobin,Saint.George's respiratory questionnaire (SGRQ) and transthoracic echocardiography.Pulmonary artery systolic pressure (sPAP) ≥ 36 mmHg(1 mmHg =0.133 kPa) was defined as PH.Results A total of 251 patients were finally evaluable in this study.The frequency of PH was 55.4％ (139/251) in patients with stable COPD.Significant differences were seen between patients with PH and without PH respectively in the following factors (mean P ＜0.05):proportion of age ≥ 60 years (69.8％ vs 57.1％),forced expiratory volume in one second (FEV1)(％ predicted) [(47.5±8.2)％ vs(61.2±10.2)％ and(49.8±7.9)％ vs(66.4±11.3)％],sPAP [(41.9 ± 9.1) mmHg vs (28.2 ± 3.2) mmHg],exercise oxyhemoglobin desaturation [(-5.5 ± 3.2) ％ vs(-2.2 ± 1.2) ％],6MWD [(316.0 ± 55.2) m vs(390.0 ±75.2) m].The following variables were negatively correlated with sPAP:6MWD (r =-0.330,P =0.003),FEV1 (％ predicted) (r =-0.210,P =0.024 and r =-0.130,P =0.012,respectively).The following variables were positively correlated with sPAP:age(r =0.560,P =0.031),exercise oxyhemoglobin desaturation ＞ 3％ (r =0.540,P =0.001).Logistic regression test has showed that age≥ 60 years,exercise oxygen desaturation ＞ 3％,FEV1 (％ predicted) ＜ 50％,6MWD ＜ 350 m were risk factors for PH in COPD.Conclusion The incidence of PH in COPD increases with age,yet the performance of lung function and the activity of endurance decrease in elder patients.Sixty years or older,exercise oxygen desaturation ＞ 3％,FEV1 (％ predicted) ＜ 50％,6MWD ＜ 350 m are risk factors of PH in COPD.Echocardiography or right heart catheterization when needed should be considered to confirm the diagnosis.

Objective To investigate the technical difficulties and the avoidance of complications in delayed laparoscopic cholecystectomy (LC) for acute cholecystitis (AC).MethodsThe results of LC carried out on 133 consecutive patients with AC between February 2004 and August 2008 were retrospectively studied.The outcomes were compared between patients who received LC for AC within 72 hours (the early group) and those after 72 hours (the delayed group).There were 34 patients in the early group and 99 in the delayed group.During LC,Calot's triangle was carefully dissected,and the relationship of the cystic duct to the CBD and common hepatic duct was clearly identified.Retrograde cholecystectomy in 2 patients was used when the Calot's triangle was poorly identified.Laparoscopic subtotal cholecystectomy was carried out in 4 patients whose inflammation or fibrosis precluded dissection of the Calot's triangle.ResultsThere was no conversion to open cholecystectomy,biliary tract injury,biliary leak,or any other intraoperative or postoperative complications.There was no 30day readmission in the 2 groups.Patients who received delayed LC had a significantly longer operation time [(44.1±5.32) vs (66.4±3.05)rnin,P＜0.01].There was no significant difference in wound infection rates in the 2 groups (1/34 2.94 ％ vs 2/99 2.02 ％,P＞0.05).ConclusionsDelayed LC was as feasible and safe as early LC in the treatment of AC.Delayed LC was technically more demanding than early LC.

ObjectiveTo evaluate treatment of type 2 diabetes mellitus (T2DM) by laparoscopic Roux-en-Y gastric bypass (LRYGB) using different amastomoses. MethodsTwenty one T2DM patients were divided into two groups:transoral EEA (OrVil) and Endo-GIA according to ways of gastrointestinal anatomosis andunderwentLRYGB. Clinicaldataincluding outcomeof operation, complications,preoperative and postoperative oral glucose tolerance test (OGTT),Homa-IR,Homa-β,blood lipid and nutrition status were analyzed.ResultsLRYGB procedures were successfully performed in all the 21 patients with no conversion to open surgery.The difference of intraoperative blood loss,postoperative recovery time between two groups was not significant.The mean operation time in OrVil group ( 126 ± 29 )mins was shorter than that in Endo-GIA group ( 156 ± 28 ) mins ( P ＜ 0.05 ),but at the same time,the mean expenditure was higher. Evaluated on three months after operation,the T2DM cure rate was 78％,and effective rate was 100％ in OrVil goup and those were 83％,100％ respectively in Endo-GIA group.No postoperative malnutrition, anemia or severe complication occurred.ConclusionsThe efficiency of laparoscopic Roux-en-Y gastric bypass using different amastomoses was same in the treatment of type 2 diabetes mellitus.The operation time was shorter in OrVil group but the expenditure was higher.

Objective To evaluate some key steps influencing success rate in process of totally laparoscopic extraperitoneal herniorrhaphy(TEPH). Methods Between October 2006 and May 2009,we performed totally laparoscopic extraperitoneal herniorrhaphy in 98 patients with like procedures and under the same condition. Results The critical procedures of the operation are related to the establishment of the extraperitoneal cleavage. We used one-way anova analysis. The operation time in different peritoneal burst sites have no significant difference (P > 0. 05), but the operation time in different burst periods have significant difference(P <0. 05). The timing at which the burst occurs significantly influence the length of operation, while the rapture sites don't influence the operation time. The conversion rate was higher and operation time was longer between the cases with the rapture occurrence in early period and in mid and late period. Postoperatively 98 patients were followed up. The follow-up rate was 100%. The median follow-up time was 18 months. Three patients complained local discomfort one month after operation, which subsided after 2 months. There was no recurrence or chronic pain case. Conclusion The burst of the peritoneum during the process of establishing extraperitoneal cleavage significantly influences the success rate of TEPH.