1.Timely monitoring of the activation of Xiaoaiping-induced cysteine aspartase 3 in human lung adenocarcinoma cells by fluorescence resonance energy transfer technology
Tongsheng CHEN ; Longxiang WANG ; Huiying WANG ; Da XING
Chinese Journal of Tissue Engineering Research 2007;11(35):7102-7105
BACKGROUND:Combination of biological and optical technique is used to study the molecular mechanism of cell proliferation and apoptosis, which has become the study hotspot in the filed of bioengineering.OBJECTIVE: The goal of this study was to study the molecular mechanism of XAP-induced apoptosis in single living human lung adenocarcinoma (ASTC-a-1) cells by using confocal fluorescence microscopy imaging and fluorescence resonance energy transfer (FRET) techniques.DESIGN: A controlled observation.SETTING: Institute of Laser Life Science (Key Laboratory of Education Department), South China Normal University.MARERIALS: This experiment was carried out in the Institute of Laser Life Science (Key Laboratory of Education Ministry), South China Normal University between October 2006 and March 2007. Human lung adenocarcinoma (ASTC-a-1) cells were cultured in our laboratory. Xiaoaiping (XAP) parenteral solution was purchased from Tonghua Shenyuan Pharmaceutical Co.,Ltd (No. Z20025869), and G418 was purchased from Huamei Biological Company. SCAT3 was provided by Professor Masayuki Miura. Auto microplate reader (infinite M200, Tecan,Austria)and mitochondrial location fluorescent probe (Mitertracker Red)was purchased from Molecular Probe Company.METHODS: ① The inhibition of XAP at different doses to ASTC-a-1 cell viability was detected by CCK-8. ② Dynamical change of caspase 3-induced by XAP was detected by confocal fluorescence microscopy imaging and fluorescence resonance energy transfer (FRET) techniques. ③The fluorescence emission spectrum of SCAT3 was detected by confocal fluorescence microscopy imaging at different time points after XAP treatment.MAIN OUTCOME MEASURES: ①Cell viability after XAP treatment. ② Dynamic change of fluorescence resonance energy transfer efficiency of SCAT3 detected after XAP treatment. ③ Dynamical change of mitochondrial morphology after XAP treatment.RESULTS: ①XAP inhibited the viability of tumor cells dose-dependently. ② XAP induced the activation of intracellular caspase 3. ③Some mitochondria became dot, and some were cracked after XAP treatment.CONCLUSION: XAP can induce the apoptosis of ASTC-a-1, and caspase 3 is involved in the regulation course.
2.The clinical analysis of 98 patients with totally laparoscopic extraperitoneal herniorrhaphy
Yiping LU ; Nengwei ZHANG ; Bier HE ; Kai LI ; Tongsheng WANG ; Chen LIU
Chinese Journal of General Surgery 2010;25(5):377-380
Objective To evaluate some key steps influencing success rate in process of totally laparoscopic extraperitoneal herniorrhaphy(TEPH). Methods Between October 2006 and May 2009,we performed totally laparoscopic extraperitoneal herniorrhaphy in 98 patients with like procedures and under the same condition. Results The critical procedures of the operation are related to the establishment of the extraperitoneal cleavage. We used one-way anova analysis. The operation time in different peritoneal burst sites have no significant difference (P > 0. 05), but the operation time in different burst periods have significant difference(P <0. 05). The timing at which the burst occurs significantly influence the length of operation, while the rapture sites don't influence the operation time. The conversion rate was higher and operation time was longer between the cases with the rapture occurrence in early period and in mid and late period. Postoperatively 98 patients were followed up. The follow-up rate was 100%. The median follow-up time was 18 months. Three patients complained local discomfort one month after operation, which subsided after 2 months. There was no recurrence or chronic pain case. Conclusion The burst of the peritoneum during the process of establishing extraperitoneal cleavage significantly influences the success rate of TEPH.
3.Clinical application of totally laparoscopic extraperitoneal herniorrhaphy
Yiping LU ; Qiyuan YAO ; Nengwei ZHANG ; Kai LI ; Tongsheng WANG ; Chen LIU
International Journal of Surgery 2009;36(12):815-818
Objective To study and summarize the successful experience and the safety, easibility, practicality and operation skills of totally laparoscopic extraperitoneal herniorrhaphy (TEP). Methods From October 2006 to May 2008,225 patients in our two hospitals were performed TEP (265 totally laparoscopic extraperitoneal hernia repairs for inguinal hernia), including 55 direct inguinal hernia, 197 indirect inguinal hernia and 13 complex inguinal hernia operations. Among the 225 patients, 185 patients had unilateral hernia and 40 patients had bilateral hernias, including 29 recurrent hernia. Results The operations were lasted for 30 to 182 minutes(the average operating duration was 48 minutes for unilateral hernia and 106 minutes for bilateral hernia). The patients stayed in hospital for 2 to 8 days(the average inpatient hospital stay was 3.0 ± 1.2 days). The most common complication was scrotum bematomas,which appeared in 18 cases. Urinary re-tention appeared in 21 cases. Inguinal pain appeared in 5 cases. Bladder injury appeared 1 case. All the pa-tients were followed up for 3 months- 3 years. There was only 1 case of recurrence. Conclusions The proce-dure of TEP is safe,with faster postoperative recover,less pain,lower incidence of pain,better comfort quality and lower recurrence rate. TEP should be the main laparoscopic procedure for inguinal hernia repair.
4.The experience in laparoscopic thyroidectomy
Yiping LU ; Nengwei ZHANG ; Yao LU ; Changhai LI ; Kai LI ; Tongsheng WANG ; Chen LIU
Chinese Journal of General Surgery 2009;24(12):969-972
Objective To discuss the operative difficulties and management in laparoscopic thyroidectomy.Method The clinical data of 169 cases undergoing laparoscopic thyroidectomy were retrospectively analyzed.Result Among 169 cases,only 2 cases were switched to open surgery.Among the other 167 cases local thyroidectomy for thyroid adenoma Was performed in 37 cases,one side subtotal thyroidectomy in 63 cases,two side subtotal thyroidectomy in 42 cases,laparoscopic subtotal thyroideetomy was done in 20 cases for Grave's disease,and radical thyroidectomy was undeRaken in 5 cases of thyroid carcinoma.Postoperatively fat colliquation occurred in 2 cases,pneumohypoderma,skin bruise and temporary vocal dullness or hoarsness in 1 each case.There was no postoperative recurrent laryngeal nerve injury,nor that of the parathyroid gland. Conclusions Laparoscopie thyroidectomy has its own intraoperative difficulties. Accurate operative skills and careful perioperative management can decrease the occurrence of complications.
5.Establishment of a mouse model of chronic renal insufficiency induced by repeated administration of cisplatin
Tongsheng HUANG ; Yun GUO ; Chen YANG ; Ning AN ; Lin YE ; Haoxuan TANG ; Xijie HUANG ; Yongzhi XU ; Qingjun PAN ; Huafeng LIU
Acta Laboratorium Animalis Scientia Sinica 2018;26(1):20-28
Objective To observe the changes of renal tubular injury and the extent of interstitial fibrosis in the C57BL/6 mouse models of chronic kidney disease(CKD),and provide experimental animal evidence for study of the pro-gression of acute kidney injury(AKI)to chronic kidney disease as well as its mechanisms. Methods Twenty-four 8-week-old male C57BL/6 mice were randomly and equally divided into control group, low-dose, medium-dose, and high-dose cisplatin groups,6 mice in each group. Mice in the cisplatin groups were administrated with 5,7 or 10 mg/kg cispla-tin by intraperitoneal injection once a week for 4 weeks. Plasma creatinine and 24-hour urinary protein were detected to as-sess the renal function. The mice were sacrificed, and plasma and kidney samples were collected for subsequent tests. Pathological changes were observed using periodic acid-Schiff(PAS)staining. To evaluate renal tubules injury, the ex-pression of kidney injury molecule 1(KIM-1)was examined by immunohistochemistry and the level of urinary N-Acetyl-β-D-glucosaminidase was detected with a commercial kit. The infiltration of CD3-positive T cells and F4/80-positive macro-phages was observed by immunohistochemistry(IHC)and immunofluorescence. The expression of collagen I and α-smooth muscle actin(α-SMA)were tested by immunohistochemistry to assess the renal fibrosis, while total kidney collagen was detected by Picrosirius red staining. Results In contrast to the normal control group,the kidney injury became more seri-ous in the cisplatin-treated mice as cisplatin concentration increased. Particularly,significant kidney damage was observed in the high-dose cisplatin group. Compared with the control group,the plasma creatinine and 24-hour urinary protein were significantly increased in the high-dose cisplatin group(P<0.05 and P<0.001)indicating impaired renal function. Mor-phologically,numerous clear vacuoles and necrosis were present in renal tubule epithelial cells in the high-dose cisplatin group. The expression of KIM-1 was markedly up-regulated and the level of urinary NAG was elevated. Infiltration of CD3-positive T cells and F4/80-positive macrophages was enhanced in the mice of high-dose cisplatin group. Data from immuno-histochemistry and picrosirius red staining showed that mice of the high-dose cisplatin group developed renal fibrosis evi-denced by markedly up-regulated expression of collagen I and α-SMA. Conclusions Repeated administration of 10 mg /kg cisplatin for 4 weeks can induce chronic renal insufficiency in mice,which may serve as a novel model for the research on underlying mechanisms of progression from acute kidney injury to chronic kidney disease.
6.Expert consensus on rehabilitation strategies for traumatic spinal cord injury
Liehu CAO ; Feng NIU ; Wencai ZHANG ; Qiang YANG ; Shijie CHEN ; Guoqing YANG ; Boyu WANG ; Yanxi CHEN ; Guohui LIU ; Dongliang WANG ; Ximing LIU ; Xiaoling TONG ; Guodong LIU ; Hongjian LIU ; Tao LUO ; Zhongmin SHI ; Biaotong HUANG ; Wenming CHEN ; Qining WANG ; Shaojun SONG ; Lili YANG ; Tongsheng LIU ; Dawei HE ; Zhenghong YU ; Jianzheng ZHANG ; Zhiyong HOU ; Zengwu SHAO ; Dianying ZHANG ; Haodong LIN ; Baoqing YU ; Yunfeng CHEN ; Xiaodong ZHU ; Qinglin HANG ; Zhengrong GU ; Xiao CHEN ; Yan HU ; Liming XIONG ; Yunfei ZHANG ; Yong WANG ; Lei ZHANG ; Lei YANG ; Peijian TONG ; Jinpeng JIA ; Peng ZHANG ; Yong ZHANG ; Kuo SUN ; Tao SHEN ; Shiwu DONG ; Jianfei WANG ; Hongliang WANG ; Yong FENG ; Zhimin YING ; Chengdong HU ; Ming LI ; Xiaotao CHEN ; Weiguo YANG ; Xing WU ; Jiaqian ZHOU ; Haidong XU ; Bobin MI ; Yingze ZHANG ; Jiacan SU
Chinese Journal of Trauma 2020;36(5):385-392
TSCI have dyskinesia and sensory disturbance that can cause various life-threaten complications. The patients with traumatic spinal cord injury (TSCI), seriously affecting the quality of life of patients. Based on the epidemiology of TSCI and domestic and foreign literatures as well as expert investigations, this expert consensus reviews the definition, injury classification, rehabilitation assessment, rehabilitation strategies and rehabilitation measures of TSCI so as to provide early standardized rehabilitation treatment methods for TSCI.