Objective To study the effects of zinc on the expression of zinc transporter-7( ZnT-7) in the proliferation of the rat growth plate chondrocytes. Methods Growth plate chondrocytes were isolated from rih cartilage of Wistar rat. The cell3 were treated with zinc chelating agent N, N, N', N'-tetrakis (2-pyridylmethyl )ethylene-diamine (TPEN) of different concentration (0,5,10 and 20 μmol/L) for 12 horns. The expression of rat growth plate chondrocytes specificity collagen type Ⅱ was detected by immunohisloehemistiy. The localization of ZnT-7 was checked by immunofluorescent staining. Real-time RT-PCR and Western blot were used to measure the expression level of ZNT-7 in the cell. Results The result of the immunofluorescence showed that ZnT-7 located in the Golgi apparatus. The expression level of ZnT-7 was slightly higher in the cells treated with 5 μ-mol/L TPEN than the control group, while it was lower in the cells treated with 10 or 20 μmol/L TPEN than the control group. Conclusion ZnT-7 locates in Golgi apparatus and maintains the zinc ion stabilization in the condition of the zinc depletion.

Objective To compare the effects of cardiopulmonary resuscitation by bending and pressing the lower extremities (BPLE-CPR) with standard cardiopulmonary resuscitation (S-CPR). Methods A multicenter prospective nonrandomized controlled study was performed. Patients with cardiac arrest (CA) treated in the emergency departments and intensive care units (ICU) of seven hospitals in Eastern China from January 2013 to February 2017 were enrolled. BPLE-CPR or S-CPR was used for resuscitation according to the patient's condition. Data registration was completed in Utstein style. The primary outcome was recovery of spontaneous circulation (ROSC) rate, and the secondary outcome included survival rate at discharge, the resuscitation time of ROSE patients, blood pressures during resuscitation, the survival rates within 24 hours and beyond 24 hours, and the cerebral performance categories (CPC) of discharged patients. Results A total of 279 patients completed data registration, including 142 in the BPLE-CPR group and 137 in the S-CPR group. ROSC rate, survival rates over 24 hours and at discharge in BPLE-CPR group were significantly higher than those in S-CPR group [ROSC rate: 63.4% (90/142) vs. 29.2% (40/137), survival rate over 24 hours: 56.7% (51/90) vs. 45.0% (18/40), survival rate at discharge: 43.0% (61/142) vs. 20.4% (28/137), all P < 0.01]. The CPR duration of ROSC patients in BPLE-CPR group was significantly shorter than that in S-CPR group [minute:10 (5, 15) vs. 20 (11, 30), P < 0.01], while systolic blood pressure during CPR was significantly higher than that in S-CPR group [mmHg (1 mmHg = 0.133 kPa): 92.0 (80.0, 110.0) vs. 73.5 (65.5, 80.0), P < 0.01]. In survival discharged patients, the proportion of CPC 1 patients in BPLE-CPR group was significantly higher than that in S-CPR group [24.6% (15/61) vs. 10.7% (3/28), P < 0.01]. Conclusion BPLE-CPR is superior to S-CPR in terms of ROSC rate and discharge survival rate. In addition, the BPLE-CPR procedure is simple and easy to expand in public. Clinical Test Registration Chinese Clinical Trial Registry, ChiCTR-TRC-13003150.

Objective: To investigate the prevalence and related factors of obesity and central obesity among 35-75 year-old population in Jiangsu Province. Methods: During 2015-2017,83 530 eligible subjects aged 35-75 years from six study sites of Jiangsu Province were interviewed and examined. The data of demography,lifestyles,disease history,height,weight and waistline were collected. Logistic regression analysis was conducted for the influencing factors for obesity and central obesity. Results: A total of 83 393 residents completed the study,with a response rate of 99.84%. The prevalence of overweight,obesity and central obesity was 43.35%（standardized rate：35.90%）,20.02%（19.48%）and 59.93%（57.03%）. The results of multivariate logistic regression analysis showed that females（OR=0.822,95%CI：0.786-0.859;OR=0.900,95%CI：0.851-0.952;OR=1.130,95%CI：1.083-1.179）,45-75 years old（OR：1.120-1.731,95%CI：1.102-1.881）,graduating from high school or above（OR：0.767-0.902,95%CI：0.721-0.943）,living in urban areas（OR：1.530-2.077,95%CI：1.284-3.007）,smoking（OR：0.724-0.855,95%CI：0.678-0.898）,drinking （OR：1.125-1.179,95%CI：1.076-1.235）,hypertension（OR：1.884-3.461,95%CI：1.821-3.613）,diabetes（OR：1.363-1.758,95%CI：1.305-1.851）, dyslipidemia（OR：1.478-1.870,95%CI：1.429-1.851）were associated with overweight,obesity and central obesity. Conclusion The standardized prevalence rates of overweight,obesity and central obesity among 35-75 year-old population in Jiangsu Province are 35.90%,19.48% and 57.03%,respectively. Gender,age,education,residence,smoking,drinking,hypertension,diabetes and dyslipidemia are related factors.

Objective To investigate the effect and mechanism of tauroursodeoxycholic acid(TUDCA)on severe acute pancreatitis(SAP)-induced liver injury in rat model.Methods Thirty SD rats were randomly divided into three groups(10 in each):shame operation group(SO group),severe acute pancreatitis group(SAP group)and tauroursodeoxycholic acid group(TUDCA group).The SAP model was established by retrogradely injecting 5%sodi-um taurocholate(STC)solution(1 mL/kg)into pancreaticobiliary duct.The TUDCA group rats were intraperi-toneally injected with TUDCA(400 mg/kg·d-1)for three days continuously fore establishing models and the SAP and SO group rats were intraperitoneally injected with the same volume of saline.Rats were sacrificed 12 hrs after modeling,and then peripheral blood and part of pancreatic and liver tissues were collected.The level of amylase(AMY),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)was detected by automatic bio-chemical analyzer.The expression of interleukin-6(IL-6)was detected by enzyme-linked immunosorbent assay(ELISA).The histo-pathological profile of pancreas and liver was examined by hematoxylin-eosin(HE)staining microscopy and liver apoptosis was observed by in situ terminal deoxynucleotide transferase labeling(TUNEL).The expression level of glucose-regulating protein 78(GRP78),protein kinase R-like endoplasmic reticulum kinase(PERK),C/EBP homologous protein(CHOP),nuclear factor-κB p65(NF-κB p65)and caspase-3 protein was determined by Western blot.Results Compared with SO group,the expression level of AMY,AST,ALT and IL-6 was increased in SAP group(P＜0.05);pancreas and liver necrosis and hepatocyte apoptosis were found to be more significant and the level of GRP78,PERK,CHOP,NF-κB p65 and caspase-3 protein increased(P＜0.05).Com-pared with SAP group,the expression of AMY,AST,ALT and IL-6 was reduced in TUDCA group and pancreas and liver necrosis and hepatocyte apoptosis were less severe.The level of GRP78,PERK,CHOP,NF-κB p65 and caspase-3 protein significantly decreased(P＜0.05).Conclusions TUDCA may have a protective mechanism to al-leviate pancreatitis-induced severe and acute liver injury in rats by reducing the occurrence of endoplasmic reticulum stree(ERS),alleviating the inflammation and apoptosis as well as inhibiting PERK signaling pathway.

Melanoma is a malignant skin tumor characterized by a propensity for early metastasis and high mortality rates. Immune checkpoint inhibitors have significantly improved the prognosis for melanoma patients, however, some individuals remain unresponsive to immunotherapy, primarily due to the immunosuppressive characteristics of the tumor microenvironment. This review summarizes recent research on the melanoma tumor microenvironment and analyzes how dynamic changes in its components influence melanoma development and the efficacy of immunotherapy. Additionally, it outlines immunotherapy strategies focused on immune checkpoint inhibitors, examines their mechanisms of action and limitations, and further investigates the effects of combining immune checkpoint inhibitors with various therapeutic modalities, including radiotherapy, chemotherapy, and targeted therapies. This study aims to provide new insights into the melanoma tumor microenvironment and the advancement of personalized precision immunotherapy.

Melanoma is a malignant skin tumor characterized by a propensity for early metastasis and high mortality rates. Immune checkpoint inhibitors have significantly improved the prognosis for melanoma patients, however, some individuals remain unresponsive to immunotherapy, primarily due to the immunosuppressive characteristics of the tumor microenvironment. This review summarizes recent research on the melanoma tumor microenvironment and analyzes how dynamic changes in its components influence melanoma development and the efficacy of immunotherapy. Additionally, it outlines immunotherapy strategies focused on immune checkpoint inhibitors, examines their mechanisms of action and limitations, and further investigates the effects of combining immune checkpoint inhibitors with various therapeutic modalities, including radiotherapy, chemotherapy, and targeted therapies. This study aims to provide new insights into the melanoma tumor microenvironment and the advancement of personalized precision immunotherapy.