Objective To approach the surgical timing for the conjoined twins, location of the separation line for the joined liver, and the separation method.Methods The common bile ducts of the conjoined twins were considered as two vertical lines, and a vertical line running parallel to the two lines was set as the separation line for the joined liver.Local blood flow blocking method was then used to separate the joined liver.Results Among all the three cases of the conjoined twins, one case was with sternoxiphopagus and the other two with thoracoabdominalpagus.All the three cases of con-joined twins shared the common livers, but each case had respectively separated gallbladders and bile ducts.They underwent the surgical separation at the age of 28 d and 96 d and 89 d successfully.Their liver sections bled rarely by blocking the local blood flow.The liver function recovered successfully af-ter the operation.All the 6 sick children recovered and were discharged from our hospital.Conclusion Porvided the conjoined twins shared the joined liver with respectively separated common bile ducts, in most cases, the injuries of the important liver vascular as well as bile ducts could be avoided when the separation line for the joined liver was selected with the common bile ducts of the conjoined twins as the longitudinal coordinate.The local blood flow blocking method only blocked the local blood flow, in-terfering to the liver blood flow in the non-operating areas rarely, which was instrumental in the recovery of the liver function and increase of the survival rate of the conjoined twins after the operation.

To investigate the effects of traditional Chinese medicine (TCM) therapy for clearing heat and resolving phlegm on systemic inflammatory response syndrome (SIRS) in acute deterioration stage of chronic obstructive pulmonary disease (COPD) and the serum procalcitonin (PCT) level.

Objective To study the BC047440 mRNA expression in human hepatocellular carcinoma tissues, and the role of BC047440 gene in the carcinogenesis and development of human hepatocellular carcinoma.Methods Specimens from 36 cases of hepatocellular carcinoma and their corresponding adjacent non-cancerous tissues were examined for BC047440 mRNA expression by polymerase chain reaction after reverse transcription(RT-PCR).Results(1)the BC047440 mRNA expression in specimens of 36 cases of hepatocellular carcinoma was higher than that in their adjacent non-cancerous tissues(0.2594?0.0928 and 0.0942?0.0443,respectively,P

Objective To investigate the feasibility of combined laparoscopic resection of gallbladder and other viscera. Methods The clinical data of 69 cases of combined laparoscopic resection of gallbladder and other viscera from January 1999 to July 2004 were reviewed retrospectively. Results The laparoscopic operation was successful in 69cases,and no case was converted to laparotomy.The average operation time was(85.5?18.6)min,and the time of return of gastrointestinal function was(27.3?6.2)h. The average hospitalization time after operation was(3.9?0.8)d.There were no operative or postoperative complications , and the therapeutic results were satisfactory. Conclusions The combined laparoscopic resection of the gall bladder and other viscera is safe and feasible. It is possible to treat gallbladder diseases and associated lesions of abdominal viscera simultaneously. The lesions of several organs can be treated at one operation. This can significantly decrease patients′ pain and lower the cost of treatment, and is worthy of wide usage.

The history and current situation of cell membrane ion-channel gating mechanism study were reviewed, with an emphasis on the application and the latest developments of kinetic model in gating mechanism study; the problems in present study and ion-channel gating mechanism kinetics model for future investigations were finally discussed.
Cell Membrane ; physiology ; Humans ; Ion Channel Gating ; Kinetics ; Models, Biological ; Research ; trends

Platelet-rich fibrin(PRF), a second-generation of platelet concentrate with stereo fibrin structure, leukocyte and platelets, can release various cytokines and growth factors to promote wound healing. PRF has been widely used in the treatment of various acute and chronic wounds in traumatic surgery, plastic surgery and stomatology. Chronic wounds are common in traumatic surgery, but conventional treatment is often ineffective. Accumulating evidences have confirmed that PRF has excellent therapeutic effect on chronic wounds. According to various methods such as different specimen, centrifugation, centrifuge tube material, and freeze-drying, the researchers tried to improve the efficiency of PRF. The progress of platelet-rich fibrin preparation technology and preservation technology are reviewed in this paper.

Chronic wounds are chronic diseases with various causes, complex pathogenesis, prolonged disease and seriously affecting the quality of life of patients. Routine surgical treatment is often not effective, causing huge economic burden to patients′ individuals, families and society. In recent years, with the deepening of research, the application of platelet-rich fibrin (PRF) in chronic wound treatment has attracted more and more attention at home and abroad. This paper aims to review the development of PRF, analyzing the underlying mechanisms of promoting chronic wound healing, and deeply explore its practical application in various types of chronic wound treatment, in order to provide new ideas and strategies for the treatment field of chronic wound.