Objective To explore the application of SCPC in the apexification.Methods 153 teeth with incompletely developed root were treated with SCPC in the apexification.All of the teeth were followed up for 0.55years.Results 85% of the treated teeth got the good clinical effects of root development and apical closure.Conclusion SCPC is an ideal material to use in the apexification.

Objective:To master the current status of iodine nutrition of residents in Tongchuan City and provide basis for policy adjustment of relevant department.Methods:In 2019, the cross-sectional survey method was used to divide 5 sampling areas in the 4 districts (counties) of Tongchuan City according to the east, west, south, north, and middle, and each area sampled 1 township (street, including at least 1 street), each township (street) selected one elementary school, each elementary school selected 42 non-boarding students aged 8 - 10, and each township (street) selected 21 pregnant women as the survey subjects. Home salt samples and urine samples were collected, salt iodine and urinary iodine were monitored, and thyroid examinations were performed on students.Results:A total of 1 260 salt samples were collected, including 2 non-iodized salt and 1 240 qualified iodized salt; the median salt iodine was 24.32 mg/kg; the coverage rate of iodized salt, the qualified rate of iodized salt, and the edible rate of qualified iodized salt were 99.84% (1 258/1 260), 98.57% (1 240/1 258) and 98.41% (1 240/1 260), respectively. A total of 840 urine samples were collected from students, the median urinary iodine was 196.19 μg/L. The medians urinary iodine of students aged 8, 9, and 10 were 182.59, 222.16, and 190.36 μg/L, respectively. The median urinary iodine of male and female students were 211.27 and 186.76 μg/L. A total of 840 students were tested for thyroid, thyroid rate was 1.79% (15/840) by B-ultrasound. A total of 420 urine samples were tested of pregnant women. The median urinary iodine was 155.05 μg/L. The medians urinary iodine of pregnant women in the first, second and third trimester of pregnancy were 166.79, 176.11 and 129.82 μg/L, respectively. There was a statistically significant difference in urine iodine content of pregnant women in different pregnancy periods ( H = 9.317, P < 0.05). Conclusions:The iodine nutrition level of residents in Tongchuan City is generally suitable. Pregnant women's urinary iodine shows iodine deficiency in the third trimester. Monitoring of iodine nutrition of pregnant women should be strengthened, classified guidance and scientific iodine supplementation should be adhered, to ensure the appropriate level of iodine nutrition for pregnant women.

Objective:To determin the effect of PLGA microspheres loading with PTH(1 34)[PTH(1 34)/PLGA]on the differentiation of MC3T3E1 cells.Methods:MC3T3E1 cells were divided into control group,continuous or intermittent PTH(1 34)adminstration groups,PLGA microsphere group and PTH(1 34)/PLGA group.Osteogenesis differentiation was observed by alkaline phosphatase activity(ALP),alizarin red staining and RTPCR.Results:The PTH(1 34)/PLGA with 1 0 -9 mol/L final release concentration enhanced ALP activity and mineralization,increased the mRNA expression of RUNX2,ALP and VEGF.Conclusion:Controlledrelease of PTH(1 34)from PLGA microspheres can promote the osteogenesis differentiation of MC3T3E1 cells.

BACKGROUND: The establishment of amniotic fluid derived stem cells (AFS) can provide an individual reserve for cell therapy in nerve degenerative diseases.OBJECTIVE: To observe the effects of Noggin and basic fibroblast growth factor (bFGF) on AFS differentiation into neural cells.METHODS: Samples of amniotic fluid were obtained through amniocentesis by ultrasound from gestational age of 16-22 weeks for routine prenatal diagnosis. AFS were obtained from the 2~(nd) trimester amniotic fluid samples by immunomagnetic beads selection using CD117 antibody, and identified the surface antigen expression by flow cytometry after amplification. The 3~(rd) generation of AFS with good growth state were induced to differentiate into nerve cells, which were divided into the blank control,based-induced, Noggin-induced and bFGF-induced groups. The induced cell morphology was observed under inverted phase contrast microscopy, and the expression of nestin, β-Ⅲ tubulin and neurofilament in the induced cells was measured by using cell immunofluorescence detection.RESULTS AND CONCLUSION: Flow cytometry analysis indicated that most of AFS cells expressed CD44 and HLA-ABC, but negative for CD45 and HLA-DR. At 2 weeks after induction, the cell morphology exhibited significant changes with increased Nestin,β-Ⅲ tubulin and NF-positive rates in the bFGF-induced group. However, it had no significant difference in the Noggin-induced group and the based-induced group. It revealed that bFGF plays a vital role in the AFS differentiated into nerve cells.

DRG payment reform is an important means to control the unreasonable growth of medical expenses, improve the quality of medical services and achieve a win-win situation among three sides of hospitals, medical insurance and patients. This study adopted the Smith policy implementation process model to analyze the difficulties in the DRG policy implementation process from four aspects(idealized policies, policy implementation institutions, target groups, and policy environment), including the deviation between policy connotations and actual needs; the interest objectives of all parties were not completely aligned, the target group lacked a sense of identity, and the social impact and technological support needed to be improved. It was suggested that optimization should be carried out from four dimensions: policy supply coordination and precision, performance evaluation and personnel literacy, target group cognitive level and participation willingness, and policy implementation environment and atmosphere, in order to synergistically promote the effective implementation of DRG policies.

Objective To analyze the iodine nutritional status and the trend of children aged 8-10 years in Tongchuan City after the implementation of the new iodized salt standard,and to provide scientific basis for prevention and treatment of iodine deficiency disorders (IDD) in the region.Methods In 2013-2015,one town was selected respectively from 5 areas (east,west,south,north,center) in 4 counties of the city.A central primary school was selected in each sampled town,42 children aged 8-10 years in every school were selected for detection of the thyroid volume by palpation,and for collection of 15 urine samples for determination of urinary iodine (42 urine samples were collected in 2015).Four villages were selected from each town,15 residents were selected to determine salt iodide content by quantitative detection.Urinary iodine was tested using arsenic cerium catalytic spectrophotometry (WS/T 107-2006).Salt iodine was tested using direct titration method (the arbitration method was adopted for quantitative determination in the case of Sichuan salt or other special salts,GB/T 13025.7-2012).Results The thyroid palpate welling rate was 3.40％ (29/854),4.52％ (38/840) and 2.98％ (25/840) in children aged 8-10,respectively,and there was no statistical significant difference between different years (x2 =3.078,P ＞0.05).Totally,1 320 urine samples were collected from 8-10 years old children,the median of urinary iodine (MUI) was 185.14 μg/L;in the 3 years,the MUI in each year was 229.43,183.34 and 173.80 μg/L,respectively.The proportion of urinary iodine under 50 μg/L was less than 20％,under 100 μg/L was also far below 50％;urinary iodine proportion in 100 ～ ＜ 200 μg/L rose year by year.There were significant differences in the MUI among the 8 and 9 years age groups (H =12.736,10.128,P ＜ 0.05),there was no significant difference in the 10 years age group (H =3.849,P ＞ 0.05).In gender groups,there was significant difference in the MUI among male children (H =9.261,P＜ 0.05),there was no significant difference in the MUI among female children (H =4.759,P ＞ 0.05).The median of salt iodine was 24.10,24.75 and 24.10 mg/kg,respectively.The coverage rates of iodized salt were all higher than 95％,the qualified rates of iodized salt were all higher than 90％.Conclusions After implementation of the new standard iodized salt,the iodine level of children aged 8-10 years is at the appropriate level.The IDD surveillance indicators all meet the national standards for elimination of the disease.