Objective To establish HPLC method for fast, effective, simultaneous determination of Vancomycin and Norvancomycin in serum. Methods The separation was achieved on the Hypersil ODS2(250 mm ×4.6 mm,5μm)with methanol-acetonitrile-a mixture of potassium phosphate (9.5:2.5:88) as the mobile phase.The flow rate was 1.0 mL/min.The UV detecting wavelength was 280 nm.The temperature of the column was 35 ℃. Vancomycin and Norvancomycin were used as the internal standard for each other .Results Vancomycin and Norvancomycin blood concentration were in well linearity in the range of 1.25-100μg/mL,R2 =0.9996.The average method recovery rate of three concentrations were 96.88%,99.50%,105.01%respectively, the average method recovery rate of three concentrations were 94.40%,103.90,103.81%respectively, the intra-day and inter-day RSD were less than 5%.Conclusion The HPLC method is proved to be simple,sensitive, accurate and precise in which little blood sample is taken, thus it is suitable for the therapeutic drug monitoring(TDM) and pharmacokinetic study of Vancomycin and Norvancomycin.

Autophagy is a process for the degradation of long half-life proteins and organelles via the lysosome system. A large body of evidence indicates that the autophagic phenomena exist in the progressive atherosclerotic plaques. Vascular smooth muscle cells, macrophages, and endothelial cells treated with some stimulants that result in atherosclerosis formation in in vitro experiments, these cells show certain autophagic features, such as myelin-like structures, accumulation of ubiquitinated inclusions in the cytoplasm, and extensive vacuolization. However, although the interests in research on autophagy are increasing, the exact role of autophagy in atherosclerosis remains unclear. Therefore, understanding the underlying cellular and molecular mechanisms of autophagy will provide a new idea for studying the mechanisms and treatment of atherosclerotic disease.

Humans ; Molecular Diagnostic Techniques ; standards ; Quality Control

The distribution features of studies on reading popularization in CNKI-covered CSSCI papers were demonstrated from the aspects of their annual distribution, core authors and institution co-occurrence network by bibliometric, visualization and contents analysis.The study hot spots in the two development periods of reading popularization were displayed in the form of knowledge map with the study contents assessed in brief in order provide certain reference for the creative development of reading popularization theories and practice.

OBJECTIVE:To provide reference for the further improvement of national essential medicines system. METHODS:Taking theBull's-eyecity Chengdu as an example,based on the trading areas,the existing problems in implementing essential medicine system were analyzed by qualitative and quantitative research methods,and countermeasures were put forward. RESULTS:From the point of view of quantitative data,the first level trading areas of both proportions of medical personnel per thousand ur-ban population and numbers of primary medical and health institutions per 100 thousands population in Chengdu were the lowest. From 2010 to 2012,the outpatient and inpatients increased 1.09 million people and 0.16 million beds per day in Chengdu primary health care institutions,the largest increase in the first level trading areas,and increase and decrease in the second and third level trading areas;outpatient and inpatient drug costs fell respectively 4 yuan and 109 yuan,medicine proportion declined 6.7%,in-crease in the first level trading areas,and decreased to varying degrees in the second and third level trading areas. From the point of view of qualitative interviews,the service ability got enhancement,but still could not meet the services demand,especially the pressure in the first level trading areas near center district remains heavy;drug cost was under controlled,but the subsidies needs to be increased,the per capita drug costs in the first level trading areas remains high;the drug income and medicine proportion were inhibited,especially the significant fell in the second and third level area,but the decrease of income may restrict develop-ment. CONCLUSIONS:It is suggested that more targeted drugs are needed for meeting the services demand,more sophisticated policy are needed for improving the subsidies,and more comprehensive medical conditions are needed for ensuring institutions'de-velopment.

AIM: To study the expressions and clinical significance of PDCD4 and B7-H4 in optic gliomas in children.
METHODS:We used immunohistochemistry to evaluate the expressions of PDCD4 and B7-H4 in specimens from 52 optic gliomas in children cases, and analyzed the correlation of PDCD4 and B7 - H4 expression with clinicopathological factors children optic gliomas.
RESULTS: PDCD4 expression reduced in optic glioma tissue:PDCD4 expression rate in 52 cases of optic glioma tissues decreasing was correlated with tumor malignancy increase (P<0. 01); B7-H4 positive expression increased in optic glioma tissue: B7-H4 expression rate in 52 cases of optic glioma tissues increasing was correlated with tumor malignancy increase (P<0. 01); PDCD4 expression was negatively correlated with B7-H4 expression in optic gliomas (P<0. 01).
CONCLUSION: Expressions of PDCD4 and B7-H4 may be clinically relevant to tumor malignancy of optic gliomas in children and prevention of metastasis and prediction of prognosis.

The case teaching method was applied in the course of Clinical Rehabilitation. The key factors included the case design, operation and assessment in its working program. In the teaching of Clinical Rehabilitation, the use of the case teaching method can stimulate the interest of studence and enhance the effect of teaching.

Acute ischemic stroke is characterized by high morbidity,high mortality,and high disability.At present,thrombolytic therapy is the only treatment that can improve the prognosis of patients in acute phase.This article reviews the choice of time window,the primary means of thrombolytic therapy and medications,and the complications of recanalization after thrombolytic therapy.

The incidence of gastric carcinoma is increasing and lymphatic metastasis is one of the major causes of death.Vascular endothelial growth factor-C(VEGF-C),Vascular endothelial growth factor D(VEGF-D)linking their receptor(VEGFR-3)can increase lymphangiogenesis,advance lymphatic metastasis,and relate to lymphatic vessel density of tumor.More and more studies of lymphatic vessel of gastric carcinoma cause great attention.We summarizeed the mechanism of lymphangiogenesis,the new development of relationship between lymphangiogenesis and lymphatic vessel density and their clinical significance.

BACKGROUND:One function of papaver nudicaule L is its analgesic effect. Previous studies showed that total alkaloid,extracted from papaver nudicaule,possesses observable analgesic effects. Nudicauline,separated from the total alkaloid of papaver nudicaule,is a new kind of alkaloid.OBJECTIVE:To study the analgesic effect of nudicauline and its mechanism of action.DESIGN:A randomized controlled study based on the experimental animals.SETTING:Institute of Traditional Chinese Medicine ,Chengde Medical Colege.MATERIALS:The experiment was conducted in the Institute of Traditional Chinese Medicine of Chengde Medical College (Key Laboratory for Research and Exploitation of Traditional Chinese Medicine) from August 2001 to December 2003.Totally 140 mice were included and randomly divided into 14 groups with 10 mice in each group in this experiment.METHODS: ① Study of the analgesic effect.The animals were randomly assigned to 5 groups:saline control group,and nudicauline 2.5,5.0 and 10.0 mg/kg groups as well as morphine group. ② relation with endor-phin.The animals were divided into 5 groups:saline control group,nudicauline +naloxone group,morphine group,and morphine +naloxone group. ③relation with nitrogen monoxide.The animals were divided into 4 groups:saline group,nudicauline group,nudicauline +L-arginine 400 mg/kg group,and n-nitro-L-arginine methyl ester (L-NAME) 37.5 mg/kg group.Totally 20 μL 10 g/L formalin was injected into the right hindlimbs of the mice. The pain observation item:walking freely was 0 point;being lame,could stand on the floor without moving was 1 point;could lift their feet was 2 point;could shake or lick their feet was 3 points.The observation fell into 2 phases:0 to 10 minutes after pain induction was set as the first phase,and 20 to 30minutes was set as the second phase.MAIN OUTCOME MEASURES:The pain response scores of the mice in each group under the effect of different drugs.RESULTS: ① Nudicauline reduced significantly the score of response to the pain induced by formalin in the second phase(P ＜ 0.05-0.01).The higher dosage of nudicauine had certain inhibitory effect on the pain reaction in the first phase (P ＜ 0.05).Morphine had significant inhibitory effect on the pain reaction in both phases(P ＜ 0.01). ② Naloxone could completely inhibit the analgesic effect of morphine,but had no influence on the analgesic effect of nudicauline. ③ The analgesic effect of nudicauline was partially blocked by L-arginine in the second phase and enhanced by L-NAME.CONCLUSION:Nudicauline has obvious analgesic effects,whereas opium antagonist naloxone can completely inhibit the analgesic effect of morphine,but cannot alter the effect of nudicauline,which indicates that the analgesic effect is not related to the endogenous opioid system. L-arginine can partially inhibit the analgesic effect of nudicauline while L-NAME can increase the analgesic effect of nudicauline,suggesting that the analgesic effect of nudicauline may be partially associated with the production and release of nitrogen monoxide.