BACKGROUND:Currently, bioactive glass and polylactic acid have been used in clinical dentistry and plastic surgery; however, their therapeutic outcomes are not satisfactory, because the material properties have some limitations. OBJECTIVE:To explore the cytocompatibility of oxygen plasma-treated polylactic acid and bioactive glass guided bone regeneration membrane. METHODS:Bioactive glass and polylactic acid were used as the basic materials to prepare polylactic acid membrane, polylactic acid and bioactive glass composite membrane and oxygen plasme-treated polylactic acid and bioactive glass composite membrane, al of which were used to culture MG63 cels. Cel adhesion rate, cel proliferation rate and alkaline phosphatase activity of MG63 cels on these three kinds of membranes were observed. RESULTS AND CONCLUSION: With the growth of time, in these three groups of membranes, the cel adhesion rate and cel proliferation rate were al significantly increased. Alkaline phosphatase activity showed a decreasing trend after the first increase, and reached its peak at the 7thday of culture. The cel adhesion rate and cel proliferation rate in oxygen plasma-treated polylactic acid and bioactive glass group were significantly higher than those in the other two groups, while the cel adhesion and proliferation rates in polylactic acid and polylactic acid and bioactive glass groups were similar. At the 3rd day of culture, the alkaline phosphatase activity in the polylactic acid and bioactive glass group and oxygen plasma-treated polylactic acid and bioactive glass group was significantly higher than that in the polylactic acid group. At the 7th and 14th days, there was no significant difference in the alkaline phosphatase activity among these three groups. These results show that oxygen plasma-treated polylactic acid and bioactive glass composite membrane has good biocompatibility, which can better promote cel adhesion, proliferation and matrix secretion from osteogenic cels.

Objective To explore the extent of agreement between measurements of temporal and spatial gait parameters made with portable gait analysis equipment and in the laboratory.Methods Fifteen healthy young people submitted to laboratory gait analysis using 3D motion analysis apparatus and then on the same day to analysis using the Gait Watch portable apparatus.Cadence,stride length,walking speed and step length were recorded.Intraclass correlation coefficients (ICCs) and Bland-Altman plots were used to evaluate the agreement between the two gait analyses.Results Test-retest comparisons with the Gait Watch apparatus generated ICCs for the temporal and spatial parameters ranging between 0.80 and 0.98,indicating good test-retest reliability.Bland-Altman plots comparing the two measurement systems also showed good agreement.According to paired simple t tests,the stride length,walking speed,and step length assessments with the two systems showed significant differences.All exceeded the minimum detection threshold (stride length =0.05 m,walking speed =0.12 m/s,left step length =0.03 m,right step length =0.04 m).Conclusions Measurements of cadence,stride length,walking speed and step length with the two systems yield acceptable agreement,and either can be used in clinical walking assessment.

Objective:To develop a quantitative HPLC method for the analysis of eight impurities in active pharmaceutical ingredi-ent (API) asenapine maleate. Methods:The substances were analyzed using an Inertsil ODS-3 C18 column (250 mm × 4. 6 mm, 5μm), and gradiently eluted by the mobile phase A of 0. 1% aqueous trifluoroacetic acid and mobile phase B of acetonitrile in a flow rate of 1. 0 ml·min-1 with the detection wavelength of 220 nm and the column temperature of 35℃. Results:Asenapine was separa-ted completely from the impurities. The calibration curve of asenapine was linear within the range of 0. 45-1 458 μg · ml-1 ( r =1. 000 0), and that the impurities was linear within the range of 0. 4-30. 0μg·ml-1(r>0. 999). The mean recovery of the impurities was 93. 1%-106. 7%(n=9). Conclusion:The method is simple,sensitive and reproducible with good specificity and reliability,which can be used in the quality control of asenapine maleate.

Automatic Data Processing ; Data Mining ; Humans ; Medicine, Chinese Traditional ; methods

Objective To explore the necessity of graded rehabilitation interventions for stroke by means of a retrospective analysis of the destination and functional outcome of stroke patients.Methods One hundred and twenty-one stroke patients discharged from the authors' department in a tertiary hospital after hospitalized rehabilitation treatment and continued with no guided rehabilitation training at home for a period of more than 15 days before readmission to the department were recruited as the family group,another 47 patients discharged from the same department and continued with hospitalized rehabilitation treatment in other institutions for a period of more than 15 days before re-admission to the department were recruited as the rehabilitation group.The demographic data,the patients' Barthel index scores Holden gait classifications at discharge from the authors' department and at re-admission of both groups were analyzed and compared.Results The complications in rehabilitation group were much less than in the family group.The difference of Barthel index scores between previous discharge and re-admission in family group was negatively correlated with the age of stroke onset,number of complications,and was positively correlated with the Barthel index score and Holden classification at the previous discharge.It was also found that the patients in family group would remain stable with their ADL performance and gait if their BI score was equal or above 45 and Holden classification equal or above grade 2 at the previous discharge.Conclusion Stroke patients in tertiary hospitalized rehabilitation program should be discharged either to secondary institution or family in accordance to their functional status.

OBJECTIVEThe present study investigated the effects of rapamycin on Aβ1-42-induced deficits in working memory and synaptic plasticity.

METHODSAfter bilateral hippocampal injection of Aβ1-42 and rapamycinin rats, spontaneous alternation in Y-maze and in vivo hippocampal long-term potentiation (LTP) of rats were recorded. All data were analized by two-way repeated measures analysis of variance (ANOVA).

RESULTS(Hippocampal injection of Aβ1-42 alone impaired working memory of rats; (2) Rapamycin did not affect working memory of rats, but alleviated Aβ1-42-induced working memory deficits, compared with Aβ1-42 alone group; (Aβ1-42 remarkably suppressed in vivo hippocampal LTP of fEPSPs in the CA1 region; (4) Pretreatment with rapamycin prevented Aβ1-42-induced suppression of LTP.

CONCLUSIONThese data indicates that rapamycin could protect against Aβ1-42-induced impairments in working memory and synaptic plasticity in rats.

Amyloid beta-Peptides ; adverse effects ; Animals ; Hippocampus ; drug effects ; Long-Term Potentiation ; Maze Learning ; Memory, Short-Term ; drug effects ; Neuronal Plasticity ; drug effects ; Peptide Fragments ; adverse effects ; Rats ; Sirolimus ; pharmacology

OBJECTIVETo investigate the effect of Ningxintong Granule (NXTG) on left ventricular diastolic dysfunction (LVDD) in patients with coronary heart disease (CHD) of qi-deficiency and blood-stasis syndrome in Chinese medicine.

METHODSSeventy-six CHD patients with LVDD of qi-deficiency blood-stasis syndrome were randomized into two groups. The 36 patients in the control group were treated by Western medical conventional treatment alone, and 40 patients in the treated group were treated by NXTG (Consisting of milk vetch root, pueraria root, ligustici, ilex-puhesceus, manchurian wildginger, 10 g in each package) and Western medical conventional treatment in combination, one package each time, thrice daily, 4 weeks as one therapeutic course. The left ventricular function (LVF) of all patients (including Emas, Amas, E/A, DT and IVRT) was evaluated by Doppler echocardiography before and after treatment, and the changes of clinical effectiveness and TCM syndrome effectiveness were observed.

RESULTSCompared with the control group after treatment, Emas, E/A increased, while Amas, DT, IVRT decreased showing statistical difference (P < 0.05). The therapeutic effects in the treated group were significantly better (P < 0.05).

CONCLUSIONNXTG with qi-enriching and blood-activating actions shows favorable effect in treating CHD patients with LVDD.

Aged ; Coronary Disease ; diagnosis ; drug therapy ; physiopathology ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Phytotherapy ; Qi ; Ventricular Dysfunction, Left ; drug therapy ; Ventricular Function, Left

Objective To summarize the common complications of chronic lymphocytic leukemia (CLL). Methods 203 cases of CLL patients from the Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, were retrospectively reviewed and followed up.The common complications of CLL were also summarized. Survival analysis was used to analyze the prognostic value of these common complications. Results 40 (19.7 %) patients sustained infectious complications,and the respiratory tract infections were the most common type (75.6 %). 15 (7.4 %) patients complicated with autoimmune diseases (AID), and the autoimmune hemolytic anemia(AIHA) were the most common type(31.3%). 8(3.94 %) patients suffered from secondary cancers, including lung cancer, etc.3(1.48 %) patients developed other high-grade lymphomas, 2 of them transformed to prolymphocytic leukemia (PLL), 1 of them transformed to diffuse large B cell lymphoma (DLBCL) which was called Richter syndrome. Complicating infections and secondary cancers or transformations were associated with poor prognosis.Complicating AIDs was not an adverse prognostic factor. Conclusion Infections, AIDs and secondary cancers or transformations are common complications of CLL patients.Lung is the most common infectious site, and AIHA is the most common AID type,none secondary cancer s incidence rate is specifically higher than the others. Infections and secondary cancers or transformations indicate poor prognosis.

This paper presents a machine learning method to select best geometric features for deformable brain registration for each brain location. By incorporating those learned best attribute vector into the framework of HAMMER registration algorithm, The accuracy has increased by about 10% in estimating the simulated deformation fields. At the same time, on real MR brain images, we have found a great deal of improvement of registration in cortical regions.
Algorithms ; Artificial Intelligence ; Brain ; anatomy & histology ; Computer Simulation ; Humans ; Image Enhancement ; methods ; Image Interpretation, Computer-Assisted ; methods ; Magnetic Resonance Imaging ; methods ; Pattern Recognition, Automated ; methods ; Reproducibility of Results

Objective To investigate the correlation between serum total bile acid level and coronary atherosclerosis. Methods The clinical data of 1408 patients who had underwent coronary angiography were retrospectively analyzed. The patients were divided into coronary atherosclerosis group (stenosis ≥ 50%, 681 cases) and coronary normal group (stenosis < 50%, 727 cases) according to the results of coronary angiography. The general clinical data, serum total bile acid, serum creatinine, fasting plasma glucose, low-density lipoprotein cholesterol (LDL-C) and so on were compared between 2 groups, and the indexes analyzed by Spearman correlation analysis and multivariate Logistic regression analysis. Results There were no significant differences between 2 groups in the sex constitution, the family history of hyperlipidemia and the history of lipid-lowering therapy (P>0.05). The rate of smoking, rate of hypertension, rate of diabetes, age, body mass index (BMI), creatinine, fasting plasma glucose, total bile acid and low density lipoprotein cholesterol in coronary atherosclerosis group were significantly higher than those in coronary normal group:18.6%(127/681) vs. 14.2%(103/727), 64.6%(440/681) vs. 45.8%(333/727), 48.5%(330/681) vs. 22.7%(165/727), (58.9 ± 12.2) years vs. (56.7 ± 13.1) years, (25.6 ± 4.3) kg/m2 vs. (24.9 ± 4.5) kg/m2, (70.28 ± 15.94)μmol/L vs. (52.79 ± 12.75)μmol/L, (6.82 ± 2.73) mmol/L vs. (5.57 ± 2.35) mmol/L, (7.86 ± 4.38)μmol/L vs. (5.63 ± 3.71)μmol/L and (3.32 ± 0.69) mmol/L vs. (2.28 ± 0.57) mmol/L, and there were statistical differences (P<0.05 or <0.01). The Spearman correlation analysis result showed that coronary atherosclerosis was positively correlated with men, age, diabetes, hypertension, BMI, serum creatinine and total bile acid (r=0.084, 0.068, 0.322, 0.263, 0.073, 0.248 and 0.176; P < 0.05 or < 0.01). The multivariate Logistic regression analysis result showed that men, diabetes, hypertension, serum creatinine, BMI ( >24 kg/m2) and total bile acid levels were risk factors of coronary atherosclerosis (P<0.05 or<0.01). Conclusions The serum total bile acid level is positively correlated with the severity of coronary atherosclerosis, which may be one of the independent risk factors for coronary atherosclerosis.