1.Diagnosis and treatment of primary splenic tumor: an analysis of 63 cases
Juan YU ; Chunfan TONG ; Huikai LI ; Qiang LI
Chinese Journal of Hepatobiliary Surgery 2010;16(10):761-763
Objective To summarize the experience in diagnosis and treatment of primary splenic neoplasm (PSN) to promote understanding of the disease. Methods The clinical data of all admitted patients with PSN treated in this hospital from February 1989 to February 2004 were retrospectively analyzed. Results A total of 63 patients were treated by surgery and pathologically confirmed as PSN. Twenty-one patients were treated by splenectomy alone. Twenty-seven patients underwent distal pancreatectomy and splenectomy. Fifteen patients received splenectomy combined with excision of tumor, the 5-year survival rate was 35.9%(15/42). Conclusion PSN should be diagnosed mainly according to clinical manifestations, image examination, and fine needle aspiration biopsy. CT and angiography play an important role in the identification of benign and malignant splenic mass. Early diagnosis, radical resection and comprehensive treatment are important for improving the prognosis of PSN.
2.Strychnine nitrate' effect on intracellular potentials of Mauthner cell evoked by skin stimulation in the crucian carps.
Li-juan ZHANG ; Xue-hong TONG ; Xiao-yi LI
Chinese Journal of Applied Physiology 2005;21(2):169-230
Animals
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Carps
;
physiology
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Galvanic Skin Response
;
drug effects
;
physiology
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Skin
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cytology
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Strychnine
;
pharmacology
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Synaptic Transmission
;
drug effects
;
physiology
3.The application and evaluation of question type and discussion type teaching during hematology probation
Xiuzhen TONG ; Juan LI ; Minghu CHEN ; Yanbing LI
Chinese Journal of Medical Education Research 2006;0(09):-
Objective To study the effect of teaching methods of question type and discussion type that are adopted during the clinical probation of hematology. Methods 200 students of 5-year system of clinical medical of zhongshan medical college of Sun Yat-sen University were sclected to use the teaching methods of question type and discussion type during the clinical probation of hematology. Results It displayed that the probation teaching methods of question type and discussion type received good evaluation from the students, and obtained good probation effect. Conclusions The problem-based teaching methods evoked and guided the students proceeding transversal and lenth clinical wise; The case discussion elevatered the students’ ability to resovle practical problems with theoretical knowledge.
4.Mechanisms of resistance to crizotinib in patients with transforming EML4-ALK fusion gene.
Hui-juan ZHANG ; Xiao-tong ZHANG ; Li ZHANG
Chinese Journal of Pathology 2012;41(12):862-864
Apoptosis
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drug effects
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Benzoquinones
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pharmacology
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Carcinoma, Non-Small-Cell Lung
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drug therapy
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metabolism
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pathology
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Drug Resistance, Neoplasm
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HSP90 Heat-Shock Proteins
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antagonists & inhibitors
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Humans
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Lactams, Macrocyclic
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pharmacology
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Lung Neoplasms
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drug therapy
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metabolism
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pathology
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Mutation
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Oncogene Proteins, Fusion
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antagonists & inhibitors
;
genetics
;
metabolism
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Protein Kinase Inhibitors
;
therapeutic use
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Pyrazoles
;
therapeutic use
;
Pyridines
;
therapeutic use
;
Triazoles
;
pharmacology
5.Blastoid mantle cell lymphoma involving the skin: a case report
Han MA ; Xiuzhen TONG ; Chang SU ; Juan LI ; Chun LU
Chinese Journal of Dermatology 2012;45(8):592-594
A 53-year-old man presented with prunosus nodules and plaques on the trunk and extremities for half a month and with periorbital swelling for 4 days.Hematological examination in a local hospital showed thrombocytopenia,and pulsed corticosteroid therapy did not work.On physical examination,there were splenomegaly and multiple enlarged superficial lymphnodes.Serum calcium and 1-lactate dehydrogenase (LDH)were increased to 3.12 mmol/L and 853 U/L,respectively.Serum immunofixation electrophoresis evidenced the presence of a monoclonal immunoglobulin IgM (κ chain).Positron emission tomography (PET-CT) showed abnormal uptake in multiple lymph nodes,back wall of the pharynx,and spleen.The biopsy of a nodule in the neck revealed a diffuse infiltration of numerous atypical lymphoid cells in the subcutaneous fat tissue,which were medium-sized with round nuclei,obvious nucleoli and karyokinesis, Immunophenotyping of the abnormal lymphocytes indicated positive reactions for L26,CD79a,Bcl-2,cyclin D1,multiple myeloma oncogene 1 (partly),Ki-67 (>80%),but negative for CD5,CD21,CD23,CD38,CD3,CD10,Bcl-6,CD45RO,terminal deoxynucleotidyl transferase (TdT),myeloperoxidase (MPO),CD30,anaplastic lymphoma kinase (ALK),CD117,or CD34.Fluorescence in situ hybridization (FISH) revealed the presence of a fusion gene (t(11:14) CCND1/IGH) in the abnormal lymphocytes.Based on the above findings,the diagnosis was made as blastoid mantle cell lymphoma with skin and periorbital involvement complicated by hypercalcemia.After treatment with rituximab injection,cyclophosphamide,vincristine,doxorubicin,dexamethasone,and intermittent treatment with intravenous high dose of methotrexate and cytarabine (R-Hyper-CVAD),serum calcium returned to a normal level three days later,and the patient made a quick and excellent recovery on the 6th day,with the regression of skin lesions and poriorbital swelling.Unfortunately,the patient eventually died of severe pulmonary infection one month later.
6.Treatment of patients with hypoparathyroidism accompanied by elevated serum muscle enzymes
Qiang TONG ; Hong LI ; Xiaozheng FANG ; Juan TAN
Chinese Journal of Endocrinology and Metabolism 2013;29(9):798-800
The clinical data of patients with hypoparathyroidism accompanied by elevated and normal muscle enzymes were analyzed retrospectively.The results showed that there were no differences in age,gender,pathogenesis,etiology,and parathormone level between groups with elevated muscle enzyme and normal muscle enzyme.Serum calcium concentration in the group with normal muscle enzyme was within normal range.Serum calcium concentration in the group with elevated muscle enzyme was significantly lower than that in the group with normal muscle enzyme (P<0.01).As the serum calcium concentration rose,the elevated enzyme level gradually returned to normal range,suggesting that the decrease of serum calcium concentration resulted in the elevated muscle enzyme levels in patients with hypoparathyroidism.
7.Suppression of Hematopoietic Stem /Pro genitor Cells by Sera from Patients with Systemic Lupus Erythe-matosus
Xiuzhen TONG ; Shaokai LUO ; Juan LI ; Wende HONG
Chinese Journal of Dermatology 1995;0(04):-
Objective To explore the mechanism of hematolo gic abnormality in patients with systemic lupus erythematosus(SLE).Methods Suppression of granulocytic and ery throid colony formation of bone marrow cells fromhealthy individuals was examined in vitro by using methylcell ulose culture with sera or IgGdelete d sera from patients with SLE.Results①Both colony-forming units of erythr ocytes(CFU-E)(in 66.7%of samples tested)and granulocytes(CFU-GM)(in 70%of samples tested)of normal bone marrowcells were sign ificantly inhibited by sera frompatients with SLE(P
8.Comparison of different detection methods of monkey B virus antibody
Jinwen LI ; Wei TONG ; Juan CAI ; Zhiguang XIANG ; Qiang WEI
Chinese Journal of Comparative Medicine 2017;27(7):29-33
Objective Monkey B virus(BV), also known as Cercopithecine herpesvirus 1,is an important zoonotic pathogen.According to the national standard, antibodies are detected using BV as an antigen.However, the preparation of BV antigen is very stricted due to biosafety issues.Therefore, in this study, we used alternative antigens to detect the BV antibody by serological assay and verified their specifity and sensitivity.Methods A total of 135 blood samples from rhesus monkeys were tested by two ELISA method (BV and HVP2) and enzyme immunosorbent assay (EIA)method.The positive and suspicious samples were verified by immuno-fluorescence assay (IFA), Western blot and immunoblotting technique using HSV-1 gC1 purified glycoprotein as an antigen.Results The positive rates of HVP2-ELISA, BV-ELISA and HSV-1-EIA were 32.6%, 37.8% and 34.8%, respectively.Consistant result of the three detection method accounted for 91.1% (123/135), and the positive result were confirmed by IFA And WB.There were 12 suspicious samples,in which 33.3% (4/12) were verified to be positive.Conclusions Compared with BV antigen, the sensitivity and specificity of the alternative antigen HSV-1 are moe close than HVP2.Positive and suspicious samples should be verified by several method to avoid missed detection.
9.The effect of triamcinolone acetonide dental paste in the treatment of erosive oral lichen planus
Mianxiang LI ; Tong WU ; Linglan YANG ; Xiaoxu LI ; Juan WANG ; Juan XIA
Journal of Practical Stomatology 2016;32(4):581-583
60 patients with pathologically diagnosed erosive lichen planus of bilateral buccal mucosa were included.The lesion on one side was treated by local application of triamcinolone acetonide dental paste(test group),the lesion on the other side without treatment was used in the control group.7,1 4,21 and 28 d after treatment the pain level and the lesion situation scores in the test group decreased significantly (P <0.001 ),and lower than those in the control group at the same time point(P <0.05).No adverse reaction was found in the 2 groups. Triamcinolone Acetonide Dental Paste is effective in the treatment of erosive oral lichen planus.
10.Vitamin K deficiency in low-birth weight premature infants and its relationship with intraventricular hemorrhage
li-ping, HAN ; hui-feng, ZHANG ; tong-juan, LI ; xiao-li, ZHANG ; wei, LIU
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To study the incidence of vitamin K deficiency in low-birth weight premature infants and its relationship with intraventricular hemorrhage.Methods We use emzymoimmunoelectrophoresis to detect prophrombin protein precursors(PIVKA-Ⅱ) in vein blood in premature infants