Object To study the difference between Radi x Codonopsis (RC) and Radix Astragali (RA) on the central nerv ous system. Methods Sleeping was induced by pentobarbital sodium and ether. The water maze was used. Results Both RC and RA could prolong the sleeping time induced by pentobarbital sodium and ether. RC and RA had effect on the scopolamine-indu ced study and memory disturbance of mice. Conclusion The effect of RC on prolonging the sleeping time induced by pentobarbital sodium and ether is better than that of RA. On the study and memory disturbance induced by sc opolamine, RC is also better tha n RA.

BACKGROUND: Lanceolata and astragalus can improve myocardial ischemia, which are widely used in clinics.OBJECTIVE: To compare the effects of extract of lanceolata and astragalus on electrocardiogram (ECG) of rats with experimental myocardial ischernia.DESIGN: Completely randomized and controlled study.SETTING: Pharmacological College of Harbin Commerce University.MATERIALS: The experiment was carried out at the Laboratory of Pharmacological College of Harbin Commerce University from September to December 2002. Totally 30 healthy adult Wistar rats were divided randomly into 3 groups: control group, lanceolata group and astragalus group with 10 in each group.METHODS: ① After anesthesia, lead ECG Ⅱ of rats was recorded.30 g/kg saline, 30 g/kg lanceolata extract and 30 g/kg astragalus extract [extract of lanceolata and astragalus (raw drug: 1 g/mL)] were injected into rats in the control group, lanceolata group and astragalus group respectively. After 1-3 minutes, 0.5 U/kg pituitrin was injected intravenously (for model of myocardial ischemia), and then saline was used to dilute from 0.1to 0.2 mL for 10-second injection. Changes of ECG after injection of pituitrin were recorded with LMA-2B Ⅱ physiological recorder (migration of T wave and ST section and change of heart rate). ② Differences of data were compared with t test.MAIN OUTCOME MEASURES: Height of T wave and Migrations of ST section and changes of heart rate of ECG of rats before and after medication.RESULTS: Data of totally 30 rats was entered the final analysis. ①Height of T wave: The height was higher after medication than that before medication in three groups (P ＜ 0.01), but that in astragalus group was obviously lower than that in lanceolata group [(1.63±3.81), (2.18±2.93) mm,P ＜ 0.01]. ② Heart rate: The rate was lower after medication than that of before medication in three groups (P ＜ 0.01), and that in astragalus group was obviously lower than that in lanceolata group [(212.21 ±5.01),(221.18±5.66) time/minute, P ＜ 0.01]. ③ Height of ST section: There were not significantly different in three groups (P ＞ 0.05), and the difference was not obvious before and after medication (P ＞ 0.05).CONCLUSION: ① Both astragalus and lanceolata can protect experimental myocardial ischemia induced by pituitrin. ② Effect of lanceolata on reduction of T-wave height is stronger than that of astragalus, but the effect of lanceolata on reduction of heart rate is weaker than that of astragalus.

AIM: The role of external-signal regulated kinaseⅠ/Ⅱ(ERKⅠ/Ⅱ) in the induction and maintenance of spinal long-term potentiation(LTP) is evaluated.METHODS: The C-fiber evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement.RESULTS:(1) PD98059(100 ?mol/L) or SL327(200(?mol/L)),a selective MEK inhibitor,completely blocked LTP induction when applied at 30 min prior to tetanic stimulation.(2) PD98059 or SL327 reversed spinal LTP in a time-dependent manner.At 15 min after LTP induction,PD98059(100 ?mol/L) or SL327(200 ?mol/L) reversed LTP completely,and at 30 min after LTP induction,the inhibitory rate of spinal LTP inhibited by PD98059 or SL327 was 62.5% and 75.0%, respectively.At 1 h after LTP induction,however,the same concentration of PD98059 or SL327 did not affect the spinal LTP.CONCLUSION: Activation of ERKⅠ/Ⅱ in spinal dorsal horn may be crucial for the induction and the early-phase maintenance of LTP of C-fiber evoked field potentials.

Objective To study the expression of CDK4 andβ-Catenin and their relevance in glioma. Methods We used immunohistochemistry to detect the expression of CDK4 andβ-Catenin in forty-five glio-ma tissues and eight normal tissues.According to the classification standard of WHO in 2000 classify and grade the tissues.Results There were significant differences of CDK4 andβ-Catenin expressions between normal tis-sues and glioma tissues(P<0.01).The expression of CDK4 and β-Catenin had positive correlation with the pathological grades of glioma and histological type and increased(P <0.05).Furthermore,the expression of CDK4 was positively correlated with the expression ofβ-Catenin in glioma(r=0.52,P<0.01).Conclusion The increased expression of CDK4 andβ-Catenin may have correlation with malignant change of glioma and oc-curance of glioblastoma,and their combination is expected to become an important indicator in assessing malignant glioma.

Objective To examine the effect of father parenting stress and paternal marital satisfaction on father involvement,and to explore the mediating role of maternal perceptions of father involvement in father involvement and maternal marital satisfaction.Methods 300 children's fathers and mothers from 5 kindergartens in Beijing city,Dezhou city,Wuhu city were surveyed with the Chinese version of the PSI/SF,the father involvement questionnaire and the marital satisfaction questionnaire.Results The score of paternal marital satisfaction was (3.98±0.75),(3.84±0.76) for maternal marital satisfaction,(2.26±0.50) for father parenting stress,(2.60±0.49) for father involvement,(2.50±0.50) for maternal perceptions of father involvement.Correlation analysis showed that father parenting stress was negatively correlated with father involvement(r=-0.36,P＜0.01),paternal marital satisfaction was positively correlated with father involvement (r=0.33,P＜0.01),father involvement was positively correlated with maternal marital satisfaction(r=0.36,P ＜0.01),father involvement was positively correlated with maternal perceptions of father involvement (r=0.48,P＜0.01).A structure equation model consisting of father parenting stress,father involvement and marital satisfaction had a better degree of fitting:GFI =0.99,NFI =0.99,CFI =0.99,RMSEA＜0.001.Maternal perceptions of father involvement mediated the effect of father involvement on maternal marital satisfaction (effect value:0.41,P＜0.05).Conclusion Father parenting stress and paternal marital satisfaction could significantly predict father involvement,father involvement could significantly predict maternal marital satisfaction.These studies verified the spillover effect and the crossover effect between spousal subsystem and parental subsystem.

Objective To evaluate the implication and necessity of deepithelialization in mastopexy.Methods A total of 124 patients with mastoptosis were randomly divided into 2 groups:group Ⅰ and group Ⅱ,62 cases each.A double-circle incision technique was used for all the patients.In group Ⅰ,full thickness skin around nipple-areola was resected.While in group Ⅱ,deepithelialization was performed and the peri nipple-areola dermis was preserved.Results The average full skin resection time was 4.5 minute per side in group Ⅰ and the skin deepithelialization time was 15.8 minute per side in group Ⅱ.Postoperative follow-up was carried out for all the 124 patients with duration of 2 weeks to 4 years.In group Ⅱ,sebaceous cysts,epidermal inclusion cyst and suture knot exclusion were found at the incision site in 8 patients (12.9 ％) at 3 weeks to 1.5 years after operation.Conclusions The blood supply to the nipple-areola complex is not affected by full-thickness skin removal during mastopexy,while the incidence of complication at the incision site decreases significantly.We conclude that deepithelialization has not much clinical significance in mastopexy.

Air ; analysis ; Chromatography, Gas ; methods ; Phenylphosphonothioic Acid, 2-Ethyl 2-(4-Nitrophenyl) Ester ; analysis ; Workplace

Object To compare the effects of Radix Codonopsis (RC) and Radix Astragali (RA) on ECG of experimental myocardial ischemia rats. Methods The model of myocardia ischemia was made by injection of pituitrin (Pit) in rats. The changes of ECG were observed with three parameters, viz. the heart rate, the locomotions of T wave and S T section. Results Both RC and RA can decrease the run up of T wave in ECG of rats with experimental myocardial ischemic injury in which RC is superior to RA. While RA has stronger effect on the slowering of the heart rate than RC.Conclusion Both RC and RA have protective effect on experimental myocardial ischemic injury induced by Pit.

BACKGROUND:Twin block appliance has been shown to effectively promote mandibular bone growth of Class Ⅱ malocclusion patients and improve lateral appearance of mandibular retrusion.The other improvement of Twin block appliance on hard-soft tissues remains unclear.OBJECTIVE:To assess the hard-soft tissue changes following Twinblock appliance treatment.METHODS:Atotal of 48 adolescent patients,30 males and 18 females,aged 11.6 years (range 9.58-12.37) with Class Ⅱ malocclusion were selected from Department of Orthodontics,Affiliated Stomatology Hospital of Hebei Medical University.Of them,27 were treated with Twinblock appliance as the treatment group,and 21 adolescent patients without treatments were selected as the control group.Cephalonetric analysis was used to compare the changes before and after treatment.RESULTS AND CONCLUSLON:Comparative analysis showed that Twinblock could effectively adjust or improve the effect of the natural growth,reduced upper lip convexity and mentolabial furrow angle.Moreover,Twinblock inhibited facial vertical distance reduction and facial convexity increase to decrease the potential differences between maxillary and mandibular bones in sagittal plance.Results show that Twinblock could effectively improve the profile of soft-tissues of Class Ⅱ malocclusion cases,

BACKGROUND:There is a high morbidity after spinal cord injury, and the therapeutic strategy is limited to early surgical intervention, medication and post-treatment exercise that only can improve the motor function slightly. However, there is no effective cure method. OBJECTIVE:To study the effect of partition-type spinal cord catheter combined with bone marrow stromal stem cels on T8 spinal cord transection damage in rats. METHODS:Fifty rats were randomized into five groups (n=10 per group): group I, T8 spinal cord transection (5 mm) was made in rats with no treatment; group II, the partition-type tube was inserted into the injured site after modeling; group III, partition-type tube combined with bone marrow stromal stem cels was implanted into the injured site after modeling; group IV, partition-type tube combined with polyglycolic acid fibers was implanted into the injured site after modeling; group V, partition-type tube combined with bone marrow stromal stem cels and polyglycolic acid fibers was implanted into the injured site after modeling. RESULTS AND CONCLUSION:At 2 and 12 weeks postoperatively, Basso, Beattie and Bresnahan scores were significantly higher in the groups III and IV than the groups I, II, IV (P < 0.05). At 12 weeks postoperatively, the latency of motor evoked potential below the injury plane was significantly decreased in group V compared with groups I, II, III, IV (P < 0.05). Immunohistochemical results displayed that in the groups III and V, regenerated nerve fibers grew positively and arranged orderly among the tubes, and there was no obvious winding phenomenon. Under transmission electron microscopy, a certain number of myelinated nerve fibers were found as bridges among groups. These findings indicate that the partition-type chitosan tube combined with bone marrow stromal stem cels has a good connection with the injured spinal cord a good connection to restore part of electrophysiological properties, accelerate the axon regeneration, recover the motor function, thereby providing a new direction for the treatment of spinal cord injury. Cite this article:Zhao XW, Liu X, Yu DP, Rong H, Yu XS, Yang CS, Liu T, Zhao TB. Partition-type spinal cord catheter combined with bone marrow stromal stem cels in the repair of spinal cord transection injury in rats. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):42-48.