Objective To establish HPLC method for fast, effective, simultaneous determination of Vancomycin and Norvancomycin in serum. Methods The separation was achieved on the Hypersil ODS2(250 mm ×4.6 mm,5μm)with methanol-acetonitrile-a mixture of potassium phosphate (9.5:2.5:88) as the mobile phase.The flow rate was 1.0 mL/min.The UV detecting wavelength was 280 nm.The temperature of the column was 35 ℃. Vancomycin and Norvancomycin were used as the internal standard for each other .Results Vancomycin and Norvancomycin blood concentration were in well linearity in the range of 1.25-100μg/mL,R2 =0.9996.The average method recovery rate of three concentrations were 96.88%,99.50%,105.01%respectively, the average method recovery rate of three concentrations were 94.40%,103.90,103.81%respectively, the intra-day and inter-day RSD were less than 5%.Conclusion The HPLC method is proved to be simple,sensitive, accurate and precise in which little blood sample is taken, thus it is suitable for the therapeutic drug monitoring(TDM) and pharmacokinetic study of Vancomycin and Norvancomycin.

Autophagy is a process for the degradation of long half-life proteins and organelles via the lysosome system. A large body of evidence indicates that the autophagic phenomena exist in the progressive atherosclerotic plaques. Vascular smooth muscle cells, macrophages, and endothelial cells treated with some stimulants that result in atherosclerosis formation in in vitro experiments, these cells show certain autophagic features, such as myelin-like structures, accumulation of ubiquitinated inclusions in the cytoplasm, and extensive vacuolization. However, although the interests in research on autophagy are increasing, the exact role of autophagy in atherosclerosis remains unclear. Therefore, understanding the underlying cellular and molecular mechanisms of autophagy will provide a new idea for studying the mechanisms and treatment of atherosclerotic disease.

Female ; Humans ; Male ; Precision Medicine ; methods ; standards ; trends ; Specialization

Autopsy ; China ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Molecular Biology ; Molecular Diagnostic Techniques ; methods ; Pathology, Clinical ; methods ; organization & administration

Dendritic Cells ; pathology ; Histiocytes ; pathology ; Histiocytosis, Langerhans-Cell ; pathology ; Humans ; Keratins ; analysis ; Langerhans Cells ; pathology ; Lymphoma, Large B-Cell, Diffuse ; pathology ; Sarcoma ; chemistry ; classification ; pathology

Biomarkers, Tumor ; Endocrine Glands ; pathology ; secretion ; Hormones ; metabolism ; Humans ; Neuroendocrine Tumors ; genetics ; pathology ; therapy ; Pathology ; methods ; trends

Antibodies, Monoclonal ; therapeutic use ; Antibodies, Monoclonal, Humanized ; Antineoplastic Agents ; therapeutic use ; Erlotinib Hydrochloride ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Neoplasms ; drug therapy ; genetics ; metabolism ; Quinazolines ; therapeutic use ; Receptor, Epidermal Growth Factor ; antagonists & inhibitors ; genetics ; metabolism ; Trastuzumab

China ; Gene Rearrangement ; Humans ; Immunohistochemistry ; Lymphoma ; classification ; diagnosis ; genetics ; pathology ; Quality Control ; Staining and Labeling ; World Health Organization

Humans ; Molecular Diagnostic Techniques ; standards ; Quality Control

Acute ischemic stroke is characterized by high morbidity,high mortality,and high disability.At present,thrombolytic therapy is the only treatment that can improve the prognosis of patients in acute phase.This article reviews the choice of time window,the primary means of thrombolytic therapy and medications,and the complications of recanalization after thrombolytic therapy.